Radiometals in Imaging and Therapy: Highlighting Two Decades of Research

Abstract

The present article highlights the important progress made in the last two decades in the fields of molecular imaging and radionuclide therapy. Advancements in radiometal-based positron emission tomography, single photon emission computerized tomography, and radionuclide therapy are illustrated in terms of their production routes and ease of radiolabeling. Applications in clinical diagnostic and radionuclide therapy are considered, including human studies under clinical trials; their current stages of clinical translations and findings are summarized. Because the metalloid astatine is used for imaging and radionuclide therapy, it is included in this review. In regard to radionuclide therapy, both beta-minus (β⁻) and alpha (α)-emitting radionuclides are discussed by highlighting their production routes, targeted radiopharmaceuticals, and current clinical translation stage.

1. Introduction

External radiation therapy is a common and effective treatment for various cancers. Radiation therapy was first applied over 100 years ago, immediately after the discovery of the X-ray [1]. Clinicians used it for the treatment of skin diseases, lupus, and other lesions [2,3,4,5]. However, due to the collateral damage and resulting side effects, including hair loss, blurry vision, and dry and itchy skin, its application stalled and triggered the need for an alternative treatment option [6].

In the last two decades, nuclear medicine, including radionuclide therapy, has observed significant growth and development: newer radiopharmaceuticals have been introduced, and their applications in imaging and targeted radionuclide therapy have been diverse [7]. Targeted radionuclide therapy (TRT) has great potential to destroy even the smallest clusters of metastatic cancer cells present anywhere in the body, which is hard to achieve with either external beam radiation therapy or surgery. Therefore, TRT has been clinically used to treat numerous malignancies, such as neuroblastoma, along with breast, thyroid, and prostate cancer [7,8,9]. Treatments using TRT have been explored with and without additional treatment options, such as surgery and chemotherapy.

Typically, therapeutic radiopharmaceuticals consist of four components: (i) a therapeutic radioactive isotope (e.g., ¹⁷⁷Lu, ⁶⁷Cu, ⁹⁰Y, ²¹²Pb, ²¹²Bi, ²²⁵Ac, ²²³Ra, etc.), (ii) a chelator, (iii) a linker, and (iv) a targeting vector, which delivers the isotope to the affected organs/tissues for treatment. The targeting vector could be a monoclonal antibody (mAb), antibody (Ab) fragment (diabody, nanobody, or single chain variable fragment), protein, aptamer, peptide, extracellular vesicle, virus, or simply a small molecule, such as an inhibitor [7]. Radiometal-based imaging and diagnostic radiopharmaceuticals can be categorized as (i) positron emission tomography (PET) radiopharmaceuticals (bioactive molecules labeled with positron-emitting [β⁺] isotopes) or (ii) single photon emission computed tomography (SPECT) radiopharmaceuticals (bioactive molecules labeled with gamma-emitting [γ] isotopes). Depending on the type of radiation-emitting isotopes, the therapeutic radiopharmaceuticals can be subdivided into (i) alpha (α) particle-emitting targeted radiopharmaceuticals, (ii) beta minus (β⁻) particle-emitting targeted radiopharmaceuticals, and (iii) Meitner−Auger electron (MAE)-emitting radiopharmaceuticals [10]. Additionally, when radiopharmaceuticals are radiolabeled with both the diagnostic (imaging) and therapeutic isotopes, which could use the same element (⁶⁴Cu/⁶⁷Cu, ⁸⁶Y/⁹⁰Y, ^44/43Sc/⁴⁷Sc) or different elements (⁶⁴Cu/²¹²Pb, ⁶⁸Ga/¹⁷⁷Lu, ⁶⁸Ga/²²³Ra), they are called “theranostic radiopharmaceuticals.” Depending on the plasma half-life of the targeting vector and the application (imaging/therapy), an appropriate radionuclide should be selected for radiolabeling. This article covers advancements made in radiometal-based diagnostic and therapeutic radiopharmaceuticals in the last two decades.

2. Diagnostic Radionuclides

PET and SPECT are radionuclide-based imaging modalities used routinely in nuclear medicine practice that fall under the category of “molecular imaging” because their radiotracers provide information about particular biological processes at the cellular and molecular levels.

• (i). PET measures the energy produced by the two gamma photons (511 keV) that result from annihilation of the positron emitted from the PET radionuclide with atomic electron [11]. The emitted gamma photons are detected with γ-cameras, also called scintillation detectors, which produce reconstructed three-dimensional images depicting the spatial distribution of radiotracers [11]. The common examples of PET probes includes [¹⁸F]FDG, [¹³N]NH₃, [⁶⁸Ga]Ga-PSMA, and [¹⁸F]NaF. Preclinical animal PET and clinical PET scanners offer spatial resolution of 1–2 mm and 6–10 mm, respectively, with high sensitivity of 10⁻¹¹–10⁻¹² mol/L. This level of sensitivity is sufficient to detect biological changes in an organ or tissue to identify the onset of a disease before anatomical changes occur [12].

• (ii). SPECT measures the single gamma photons emitted directly from γ-emitting radionuclides called SPECT radiopharmaceuticals. The conventional clinical SPECT scanners have lower sensitivity (10⁻¹⁰–10⁻¹¹ mol/L) and lower spatial resolution (7–15 mm) compared to PET scanners due to the limited performance of collimators [12,13,14]^. Despite this, SPECT is the most routinely used nuclear imaging procedure in the clinic and is less expensive compared to PET. The most common SPECT isotopes are ¹¹¹In, ^99mTc, ^123/131/125I, and ⁶⁷Ga.

Recent advances in SPECT γ-cameras, collimators, and reconstruction algorithms have enhanced the spatial resolution and sensitivity of SPECT scanners, allowing for the imaging of a wide range of isotope energy (20–300 keV) [15,16]. Nevertheless, both PET and SPECT need either computed tomography (CT) or magnetic resonance imaging (MRI) for accurate anatomical information. Interestingly, PET cannot distinguish between two different PET probes when injected simultaneously because it measures two γ-rays with the same energy (511 keV); meanwhile, SPECT does have multiplexing capabilities because each radionuclide produces different γ-rays, enabling it to image different targets simultaneously [17].

3. Therapeutic Radionuclides

As stated previously, therapeutic radionuclides emit α particles, β⁻ particles, and/or low-energy MAEs (non-energetic particles).

• (i). Beta minus emitters can be either of a high energy (⁹⁰Y, E_β⁻_max = 2.28 MeV,) or a low energy (¹⁷⁷Lu, E_β⁻_max = 496 keV), with tissue penetration ranges between 12 mm and 1.5 mm, respectively [18]. Given the long penetration depth of 0.2–12 mm and the moderate linear energy transfer (LET) radiation of ~0.2 keV/µm, β⁻ emitters are more suited to treating large-sized tumors (>0.5 cm), and they are considered the current gold standard in targeted radionuclide therapy [19,20].

• (ii). Alpha emitters emit α particles with high LET energies of 50–230 keV/µm and shorter penetration depths of 50–100 µm (i.e., 5–10 cell diameters) [21]. Alpha radionuclide-based targeted therapy is called targeted alpha therapy (TAT), and it is well suited for the treatment of hematological disease, small tumors, metastasis, and isolated cancer cells. Alpha emitters are perceived as a better therapeutic alternative to beta emitters due to their high LET and short tissue penetration range.

• (iii). Meitner−Auger electrons are low-energy electrons that can penetrate up to the subcellular nanometer range (<0.5 µm), resulting in a high LET of 4–26 keV/µm [22]. Given the low tissue penetration range and high LET in an extremely small area, MAE emitters could be highly valuable for treating metastatic cancers if delivered selectively within the nucleus of the cancer cells [22]. Figure 1 explains the difference between LET, pathlength (penetration range), and the usefulness of α and β⁻ radionuclide therapies.

4. Positron Emitters

4.1. Radioisotopes of Copper

4.1.1. General Information

Among the long list of radioisotopes of copper (Cu), ⁶⁰Cu, ⁶¹Cu, ⁶²Cu, and ⁶⁴Cu are used for diagnostic imaging, while ⁶⁴Cu and ⁶⁷Cu are applied in radionuclide therapy [23]. ⁶⁴Cu decays by both β⁺ (~17%) and β⁻ (~38%), making it applicable for PET imaging and targeted radionuclide therapy; therefore, it is considered a theranostic radionuclide [24]. In addition, ⁶⁴Cu also decays by electron capture (EC), which results in a cascade of Auger electrons [22,24]. The decay characteristics of Cu radioisotopes are mentioned in Table 1.

4.1.2. Growth and Advancement of Radiopharmaceuticals Labeled with Copper-Radioisotopes in Clinical Practice

In 1997, [⁶²Cu]Cu-diacetylbis(4-methylthiosemicarbazone), also known as [⁶²Cu]Cu-ATSM, was first discovered as a hypoxia imaging agent in a rat model of cardiac ischemia [26]. Later, other Cu isotopes, including ^60/61/64Cu, were used to radiolabel ATSM and employed for imaging of hypoxic solid tumors, with a similar uptake and clearance profile in patients [27,28,29,30,31].

Considering the longer half-life of ⁶⁴Cu, [⁶⁴Cu]Cu-ATSM was applied as a hypoxia imaging radiotracer in rectal cancer (National Clinical Trial (NCT) 03951337) [32]. However, several debatable preclinical studies highlighted its lower uptake in hypoxic tumors [28]. The therapeutic potential of [⁶⁴Cu]Cu-ATSM was first studied in 2001, where it resulted in a six-fold increase in the survival of 50% of hamsters bearing human GW39 colon cancer [33]. Later, several additional preclinical studies supported the theranostic potential of [⁶⁴Cu]Cu-ATSM to treat various colon carcinoma xenografts (Colon-26, HT-29). In addition to preclinical studies, clinical studies are needed to prove its true theranostic value [34,35,36].

During the last two decades, various ⁶⁴Cu-labeled Abs have been developed for immuno-PET imaging [37,38,39]. Among them, [⁶⁴Cu]Cu-DOTA-trastuzumab showed promising clinical utility in identifying HER2+ tumors with high sensitivity (~89%) in breast cancer patients [40,41].

Currently, commonly used somatostatin radiotracers for neuroendocrine tumor (NET) diagnosis are [¹¹¹In]In-DTPA-octreotide [42], [^99mTc]Tc-EDDA/HYNIC-TOC [43], and [⁶⁸Ga]Ga-DOTATOC [44], with first-in-human studies reported in the years 1993, 2005, and 2001, respectively. During 2012–2017, Pfeifer and Johnbeck’s team conducted two separate clinical studies using newly developed [⁶⁴Cu]Cu-DOTA-TATE on NET patients. Their findings revealed the outperformance of [⁶⁴Cu]Cu-DOTA-TATE over [¹¹¹In]In-DTPA-octreotide [45] and [⁶⁸Ga]Ga-DOTATOC [46] in terms of spatial resolution, lesion detection rate, and, most importantly, the ability to identify additional lesions.

A recent clinical study of [⁶⁴Cu]Cu-DOTA-TATE (200 MBq dose) demonstrated that [⁶⁴Cu]Cu-DOTA-TATE is excellent for lesion detection in neuroendocrine neoplasm patients [47]. In 2020, the Food and Drug Administration (FDA) approved the first ⁶⁴Cu-labeled PET radiopharmaceuticals, [⁶⁴Cu]-DOTA-TATE (Detectnet^TM), for the localization of somatostatin-targeting receptor (SSTR)-positive NETs in adult patients [48]. Additionally, the radiotracer [^67/64Cu]Cu-Sar-TATE was recently entered into multiple clinical trials to diagnose and treat SSTR-positive tumors [49].

In addition to radiolabeled somatostatin-targeting peptides, a series of PSMA ligands have been identified and radiolabeled with Cu radioisotopes for clinical diagnosis and radionuclide therapy applications in PCa [50,51,52]. In 2016, ⁶⁴Cu-labeled PSMA-617 became the first ⁶⁴Cu-labeled ligand for PET imaging of PCa patients and was investigated at two nuclear medicine centers (Vienna, Austria, and Bed Berka, Germany) [53]. Even though [⁶⁸Ga]Ga-PSMA is an excellent tracer to detect PCa and metastatic lesions in the lymph node or bone at low PSA levels [54], the advantage of the lower positron energy of ⁶⁴Cu (E_β⁺_avg = 278 keV) vs. ⁶⁸Ga (E_β⁺_avg = 829 keV) and the longer half-life (12.7 h) of ⁶⁴Cu allow its distribution and use as [⁶⁴Cu]Cu-PSMA-617 at various clinical PET centers with no sophisticated onsite radiotracer production facility [53].

⁶⁷Cu is one of the most promising radionuclides for radioimmunotherapy (RIT), as its 61.8 h isotopic half-life is well matched with the residence time of a typical Ab on the tumor site. In 1998, DeNardo reported a pilot study of [⁶⁷Cu]Cu-2IT-BAT-Lym-1 to image and treat chemo-resistive B-cell in non-Hodgkin’s lymphoma while employing favorable SPECT imaging and the remarkable radiotherapeutic effects of ⁶⁷Cu-labeled 2IT-BAT-Lym-1 [55]. The clinical investigation of Cu radiopharmaceuticals is outlined in Table 2.

4.1.3. Production and Availability

At present, the most common method to produce ⁶⁴Cu is proton irradiation of enriched ⁶⁴Ni via a ⁶⁴Ni(p,n)⁶⁴Cu nuclear reaction in a small−medium-energy biomedical cyclotron [66]. The main route to produce ⁶⁷Cu for decades had been via a ⁶⁸Zn(p,2p)⁶⁷Cu nuclear reaction that utilizes enriched ⁶⁸Zn and high-energy proton irradiation (up to 40 MeV), which also coproduces ⁶⁴Cu [67]. Recently, Mou et al. developed and patented the fabrication of multi-layer targets composed of enriched ⁷⁰Zn and ⁶⁸Zn that could maximize ⁶⁷Cu production yield [68].

4.2. Radioisotopes of Gallium

4.2.1. General Information

Among the many radioisotopes of Gallium (Ga), ⁶⁶Ga, ⁶⁷Ga, and ⁶⁸Ga are predominantly used in medical applications for the radiolabeling of various biomolecules [69]. ⁶⁷Ga and ⁶⁸Ga are predominantly used in nuclear medicine for SPECT and PET imaging, respectively. ⁶⁶Ga ((t_1/2 = 9.49 h) is an attractive PET radionuclide with a relatively longer half-life than ⁶⁸Ga (t_1/2 = 67.71 min). Due to its high positron emission energy (E_β+avg = 1750 keV), though, along with the co-emission of higher gamma rays than ⁶⁸Ga, ⁶⁶Ga suffers from poor image resolution and high radiation exposure to workers, limiting its medical application [70].

⁶⁷Ga is one of the longer-lived Ga radioisotopes, and it decays by EC (100%) with multiple gamma emissions, with the most common gamma energies emitted as 93 keV (39%), 184 keV (21%), and 300 keV (17%) for the SPECT imaging [71]. [⁶⁷Ga]Ga-citrate is the most popular radiopharmaceutical of ⁶⁷Ga. For several decades, it has been used in the diagnosis of osteomyelitis and other bone infections [72,73]. To date, [⁶⁷Ga]Ga-citrate scintigraphy is used worldwide for the diagnosis of lymphomas [74], lung cancer [75,76], and inflammation of the kidneys [77]. The nuclear decay properties of Ga radionuclides are displayed in Table 3.

4.2.2. Clinical Practice

Gallium-68 is one of the earliest radionuclides applied in the early days of PET scans (early 1960s), long before the discovery of [¹⁸F]fluorodeoxyglucose (FDG) in 1978 [79]. However, the growth of ⁶⁸Ga-labeled radiopharmaceuticals in clinical applications began after the commercial launch of next-generation ⁶⁸Ge/⁶⁸Ga generators in the early 21st century (mid-2000s) [80,81]. During 2000–2010, various Ga-68-labeled peptide-based radiopharmaceuticals, such as [⁶⁸Ga]Ga-DOTA-TATE [82], [⁶⁸Ga]Ga-DOTA-TOC [44,82], and [⁶⁸Ga]Ga-DOTA-NOC [83], were clinically evaluated in peptide receptor radionuclide therapy (PRRT) to visualize NET-expressing SSTR2. Later, [⁶⁸Ga]Ga-PSMA-11 (also known as PSMA-HBED, HBED-CC) was investigated for the diagnosis of recurrent PCa, and it received FDA approval in 2020 [84]. Several clinical studies involving ⁶⁸Ga were recently conducted with its “theranostic twin”, ¹⁷⁷Lu, for diagnosis and radionuclide therapy of NETs and PCa. A tremendous growth in the application of [⁶⁸Ga]Ga-PSMA-11 has occurred for imaging metastatic castration-resistant PCa over other radiotracers. Additionally, there is high demand for its theranostic pair of PSMA, labeled with either beta emitter ¹⁷⁷Lu or alpha emitter ²²⁵Ac [85,86,87,88]. The clinical investigation of ⁶⁸Ga radiopharmaceuticals is noted in Table 4.

4.2.3. Production and Availability

Currently, the most convenient method to produce ⁶⁸Ga is from germanium (Ge) 68 (t_1/2~271 d) using ⁶⁸Ge/⁶⁸Ga generators [89]. However, the shortage of these generators and on-demand supply of ⁶⁸Ga have led to the generation of alternative methods of production using, as an example, cyclotrons (12–17 MeV) via the ⁶⁸Zn(p,n)⁶⁸Ga nuclear reaction [90]. During 2014–2019, Pandey et al. pioneered cyclotron-mediated ⁶⁸Ga production using a liquid target to overcome the global shortage of ⁶⁸Ga [91,92,93]. Besides liquid target-based production, several high-yielding solid target-based production methods, also using cyclotron, have been developed and commercialized to meet the upcoming demands of ⁶⁸Ga [94,95,96]. On the other hand, the common production of ⁶⁷Ga through the irradiation of ^natZn or isotopically enriched ⁶⁸Zn targets via ⁶⁸Zn (p,2n)⁶⁷Ga or ⁶⁷Zn(p,n)⁶⁷Ga on cyclotron have been reported [97].

Table 4

Clinical applications of ⁶⁸Ga-labeled radiopharmaceuticals.

Radiopharmaceuticals	Biological Target	NCT Number ^	Disease
[68Ga]Ga-PSMA-11	PSMA	NCT03207139 (Phase II; completed) NCT03982407 (Early Phase I; completed)	Latent prostate cancer [98], hepatocellular carcinoma [99]
[68Ga]Ga-NGUL/[177Lu]Lu-DGUL	PSMA	NCT05547061(Phase I/II; ongoing)	Metastatic castration-resistant prostate cancer [100]
[68Ga]Ga-DOTA-TATE vs. [68Ga]Ga-DOTA-TOC	SSTR2	NCT04298541 (Phase II; ongoing)	Meningioma [101]
[68Ga]Ga-DOTA-TOC	SSTR	NCT02441062 (Phase II; completed)	Neuroendocrine tumors [102]
[68Ga]Ga-FAPi-46	FAPI	NCT04457258 (Early Phase I; ongoing)	Sarcoma, recurrent or metastatic, sarcoma [103]

NCT: National clinical trial, NETs: Neuroendocrine tumors, PSMA: Prostate-specific membrane antigen, SSTR2: Somatostatin receptor 2, FAPI: Fibroblast activation protein inhibitor, ^ clinicaltrials.gov and data accessed on 15 August 2023.

4.3. Radioisotopes of Zirconium

4.3.1. General Information

Zirconium-89 (⁸⁹Zr) is a promising radionuclide for the PET imaging of Abs due to its longer physical half-life (78.4 h), which matches with the blood half-life of most full-length Abs (days to weeks) [104]. ⁸⁹Zr has a relatively short penetration range by emitting low-energy positrons (E_β⁺_avg = 396 keV), which facilitate high-resolution PET images [104]. However, ⁸⁹Zr emits an abundance of high-energy γ-rays of 909 keV, adding radiation exposure to medical staff and patients [104]. Table 5 summarizes the decay characteristics of Zr-89.

Table 5

Decay characteristics of Zirconium-89 ^#.

Isotope	Half-Life (t1/2)	Decay Characteristics	EnergyEβ+avg (keV)	Eγ; keV (Intensity%)
89Zr	78.41 h	β+ = 22.3%	395.5	511 (45.5)
		EC = 76.6%		909.2 (99)

^# Data on ⁸⁹Zr are from [105]. Please refer to decay Scheme 1A.

4.3.2. Clinical Practice

In 2006, the first clinical study of [⁸⁹Zr]Zr-immuno PET was reported, where ⁸⁹Zr-labeled chimeric mAb U36 localized in all primary tumors and lymph node metastasis of head and neck cancer patients with an accuracy as high as 93% [106]. Presently, the FDA has approved hundreds of mAbs against various biological targets, such as HER2, CD20, epidermal growth factor receptor (EGFR), vascular endothelial growth factor (VEGF), and PSMA, resulting in several [⁸⁹Zr]Zr-Immuno PET-based clinical oncology trials [107,108]. Currently, [⁸⁹Zr]Zr-trastuzumab and [⁸⁹Zr]Zr-pertuzumab are the two common choices for immuno-PET-targeting HER2+ breast cancer. The first-in-human studies using [⁸⁹Zr]Zr-trastuzumab (37 MBq) and [⁸⁹Zr]Zr-pertuzumab (74 MBq) on metastatic breast cancer patients were reported in the years 2010 and 2018, respectively [109,110]. Currently, both radiopharmaceuticals are registered in clinical trials.

Additional clinical pilot studies have been published using [⁸⁹Zr]Zr-immuno-PET probes, such as [⁸⁹Zr]Zr-bevacizumab targeting VEGF-A expression [111], [⁸⁹Zr]Zr-rituximab targeting B-lymphocyte antigen (CD20) expression [112], and [⁸⁹Zr]Zr-cetuximab-targeting EGFR [113], in various tumors. These [⁸⁹Zr]Zr-immuno-PET probes have been shown to be useful for imaging and/or radionuclide therapy applications. Currently, [⁸⁹Zr]Zr-bevacizumab is registered in an ongoing clinical trial (National Clinical Trial (NCT) 01894451) [114]. Considering the long blood circulation time of monoclonal Abs (mAbs), alternative Abs and their fragments were developed in the last five years to significantly shorten their retention time in blood and to rapidly clear the unbound fragments from the body [115]. Early examples of Ab fragment application are minibody-based radiopharmaceuticals, such as [⁸⁹Zr]Zr-Df-IAB2M, which were used to detect PSMA-positive PCa and recurrent cerebral high-grade gliomas [116,117]. In addition to minibody-based radiopharmaceuticals, several preclinical studies reported ⁸⁹Zr-labeled affibodies, such as [⁸⁹Zr]Zr-Df-ZEGFR:03115 and [⁸⁹Zr]Zr-DFO-MAL-Cys-MZ, as targeting EGFR and HER2, respectively [118,119]. To fully evaluate the clinical value of affibodies and Ab fragments, additional clinical studies demonstrating their usefulness are paramount.

Besides Abs, cell labeling with ⁸⁹Zr has been explored for the imaging of white blood cells and CAR-T cells. Several preclinical studies using [⁸⁹Zr]Zr-oxine and [⁸⁹Zr]Zr-Df-aTCRmu-F(ab’)₂ were reported to track T cells in glioblastoma and acute myeloid sarcoma, respectively [120,121]. Covalent tethering of [⁸⁹Zr]Zr-DBN to cells is another highly studied methodology to noninvasively track various cell types with PET. Published reports of this method demonstrate that it offers a robust and reliable approach that could be translated in humans for monitoring cell-based therapies [122,123,124,125]. Table 6 summarizes the clinical applications of ⁸⁹Zr-based radiopharmaceuticals.

4.3.3. Production and Availability

The production and availability of ⁸⁹Zr have been improved significantly in the last two decades. Various methods of ⁸⁹Zr production on solid and liquid targets using cyclotron have evolved over the years, resulting in better and simplified methods of purification and radiolabeling [91,93,126,127,128,129,130,131,132]. The main route of production is proton irradiation of yttrium (Y) via a ⁸⁹Y(p, n) ⁸⁹Zr nuclear reaction [126]. At present, ⁸⁹Zr is routinely produced at various academic institutions for their own use, including Mayo Clinic Rochester, and for supplying other institutions, including the University of Wisconsin, the University of Alabama, and commercial vendors within the United States. Some European and Asian academic institutions also manufacture ⁸⁹Zr routinely and use it predominantly in preclinical studies. In the last 10 years, several groups have come up with alternative solutions that facilitate the GMP-grade production and formulation of ⁸⁹Zr. For example, Wooten et al. designed an automated system for routine ⁸⁹Zr production and purification at high radioactivity quantities, with >99.9% of radionuclidic purity [133]. In terms of purification, Pandey et al. developed a simplified synthesis of hydroxamate resin for trapping of Zr-89 with a trapping efficiency of 93% and its subsequent elution either as oxalate or phosphate in a high elution efficiency (>90%) [134]. Recently, the same group designed a new solid target insert and optimized the thickness of ⁸⁹Y foil and proton beam energy to improve the production yield of ⁸⁹Zr (~129 mCi or 4.77GBq) using medium energy cyclotrons [126]. Others in the field have also significantly contributed towards the advancement of Zr-89 production and purification [135,136].

Table 6

Clinical applications of ⁸⁹Zr-labeled radiopharmaceuticals currently under investigation.

Radiopharmaceuticals	Targets	NCT Number ^	Disease
[89Zr]Zr-Df-hJ591	PSMA	NCT01543659 (Phase I/II; ongoing)	Prostate cancer [137]
[89Zr]Zr-Df-IAB2M	PSMA	NCT02349022 (Phase II; completed)	Prostate cancer [138]
[89Zr]Zr-Df-IAB22M2C	CD8+Tlymphocytes	NCT05013099 (Phase IIb; ongoing)NCT03107663 Phase I; completed)NCT03802123 (Phase II; completed)	Melanoma [139]Renal cellcarcinoma [140]Metastatic solid tumors [141]
[89Zr]Zr-daratumumab	CD38	NCT03665155 (Phase II; completed)	Multiple myeloma [142]
[89Zr]Zr-trastuzumab	HER2+	NCT01420146 (Phase I; completed)	Breast neoplasm [143]
[89Zr]Zr-ss-pertuzumab	HER2-	NCT04692831 (Phase I; ongoing)	Breast carcinoma [144]
[89Zr]Zr-bevacizumab	VEGF	NCT01894451 (Early Phase I; completed)	Inflammatory breast carcinoma [114]
[89Zr]Zr-panitumumab	EGFR	NCT03733210 (Phase I; completed)	Carcinoma of head and neck [145]
[89Zr]Zr-cetuximab	EGFR	NCT00691548 (Phase I; completed)	Stage IV cancer [146]
[89Zr]Zr-girentuximab	Carbonic anhydrase	NCT03849118 (Phase III; completed)	Renal cell carcinoma [147]
[89Zr]Zr-durvalumab	PDL-1	NCT03853187(Phase II; completed)	Non-small cell lung cancer [148]
[89Zr]-DFO-atezolizumab	PDL-1	NCT04006522 (Phase II; ongoing)	Renal cell carcinoma [149]

NCT: National clinical trial, PSMA: Prostate-specific membrane antigen, CD38: Cluster of differentiation 38, HER2: Human epidermal growth factor 2, VEGF: Vascular endothelial growth factor, EGFR: Epidermal growth factor receptor, PDL-1: Programmed cell death ligand-1, ^ clinicaltrials.gov and data accessed on 15 August 2023.

4.4. Radioisotopes of Scandium

4.4.1. General Information

Scandium (Sc) has 25 different radioisotopes, but ⁴³Sc, ⁴⁴Sc, and ⁴⁷Sc are the commonly explored radionuclides for PET imaging and targeted radionuclide therapy applications [150]. ⁴⁴Sc and ⁴³Sc are promising PET radionuclides, and they are superior alternatives to ⁶⁸Ga because of their lower positron energy and almost 3.5-fold longer half-life [150,151]. However, ⁴⁴Sc also decays via high gamma ray (E_γ = 1157 keV; 99.9% abundance) emission and could give a high radiation exposure dose compared to other competing PET radionuclides [152]. The decay properties of Sc radionuclides are given in Table 7.

4.4.2. Current Clinical Application of Scandium-44

In 2017, the first clinical study of generator-derived ⁴⁴Sc with [⁴⁴Sc]Sc-DOTATOC was reported for the imaging of a metastatic neuroendocrine neoplasm at Bed Berka [153]. Recently, [⁴⁴Sc]-PSMA-617 was also applied for the imaging of PCa patients [154], and it showed performance comparable to [⁶⁸Ga]Ga-PSMA-617 in terms of tumor uptake and image quality [154]. Given the availability of both imaging (^43/44Sc) and therapeutic (⁴⁷Sc) radionuclides, Sc radiopharmaceuticals are gaining significant interest as an alternative theranostic pair [155].

⁴³Sc is another PET isotope of the Sc family with similar physical characteristics to ⁴⁴Sc, but it is devoid of high-energy gamma emission and lower positron energy (E_β⁺_avg = 476 keV), making it a more favorable imaging isotope than ⁴⁴Sc [150]. However, no preclinical or clinical studies are yet reported with ⁴³Sc-labeled radiopharmaceuticals.

⁴⁷Sc is a radio theragnostic isotope that emits low-energy β⁻ particles (E_β⁻_avg = 162 keV) and low-energy γ-radiations (E_γ = 159 keV) [150]. The decay characteristics of ⁴⁷Sc are like ⁶⁷Cu (E_β-avg = 141 keV, E_γ = 184 keV) and ¹⁷⁷Lu (E_β⁻_avg = 134 keV, E_γ = 113, 208 keV). Recently, a comparative preclinical study with [⁴⁷Sc]Sc-folate (12.5 MBq), [¹⁷⁷Lu]Lu-folate (10 MBq), and [⁹⁰Y]Y-folate (5 MBq) showed a similar therapeutic response in an ovarian xenograft model [156]. Due to the challenging production routes of ⁴⁷Sc, though, clinical studies involving ⁴⁷Sc have yet to evolve [156].

4.4.3. Production and Availability

In 2010, Frank Rosch et al. reported the production of ⁴⁴Sc (approx.185 MBq) using a ⁴⁴Ti/⁴⁴Sc generator for the first time at Bed Berka, Germany [157]. However, the production of the parent radionuclide, ⁴⁴Ti, and the accessibility of these generators were challenging. Later, in 2015, Van der Meulen et al. reported a cyclotron-based production of ⁴⁴Sc via proton irradiation (11 MeV) of an enriched ⁴⁴Ca target, which allowed elution of approximately 2 GBq of ⁴⁴Sc at the Paul Scherrer Institute (PSI) in Switzerland [158]. Later, Szkliniarz et al. reported several cyclotron-based production routes for emerging ⁴³Sc using either α particles or deuteron beams because the limited availability of high-energy multi-particle cyclotrons restricted the utility of these production routes [159]. Van der Meulen et al. demonstrated the production of 480 MBq of ⁴³Sc using enriched ⁴³CaCO₃ and targets via a ⁴³Ca(p,n)⁴³Sc reaction; limited purity of ⁴³Sc was obtained due to the co-produced mixture of ⁴³Sc (66.2%) and ⁴⁴Sc (33.3%) [160]. ⁴⁷Sc can be produced using a cyclotron [161], neutron flux reactor [162], or electron linear accelerator [163,164].

4.5. Radioisotopes of Terbium

4.5.1. General Information

Among the various radioisotopes of Terbium (Tb), four (^{149/152/155/161}Tb) are of great interest in nuclear medicine and are commonly referred as a “Swiss army knife” of nuclear medicine [165]. ¹⁴⁹Tb has both positron and α-emission properties for both PET and targeted therapy applications [166]. ¹⁵²Tb is another positron emitter with a relatively longer half-life of 17.5 h that could be utilized for radiolabeling of large biomolecules [166]. The decay characteristics of Tb radionuclides are listed in Table 8.

In 2012, Muller et al. performed the first radiolabeling of albumin-binding folate conjugates (cm09) with ^{149/152/155/161}Tb in an FR-positive tumor xenograft mouse model [167]. The findings demonstrated excellent tumor visualization through PET/CT using [¹⁵²Tb]Tb-cm09 and SPECT/CT, using both [¹⁵⁵Tb]Tb-cm09 and [¹⁶¹Tb]Tb-cm09 probes at 24 h post administration. On the other hand, α therapy version [¹⁴⁹Tb]Tb-cm09 and β⁻ therapy version [¹⁶¹Tb]Tb-cm09 resulted in significantly delayed tumor growth by 33% and 80%, respectively.

4.5.2. Preclinical and Clinical Applications

• (i). ¹⁴⁹Terbium: ¹⁴⁹Tb represents one of the powerful candidates for TAT, which emits short penetrating (~25 µm range) α particles (Eα = 3.97 MeV; I_α = 16.7%) compared to currently employed α emitters [168]. Because ¹⁴⁹Tb also decays by positrons (β⁺) and γ-radiations, ¹⁴⁹Tb-labeled radiopharmaceuticals could also be useful for PET and SPECT imaging [168].

In 2004, Beyer et al. demonstrated the first preclinical RIT with [¹⁴⁹Tb]Tb-rituximab in a leukemia xenograft mouse model, which resulted in tumor-free survival >120 days among 89% of the [¹⁴⁹Tb]Tb-rituximab-treated mice [169]. However, nearly 28% of the residual radioactivity of longer-lived daughter nuclides, ¹⁴⁹Eu (t_1/2 = 93 d),¹⁴⁵Sm (t_1/2 = 340 d), and others were retained mainly in the mice bone marrow. Baum et al. demonstrated the first in-man PET/CT study of SSTR-targeted [¹⁴⁹Tb]Tb-DOTANOC on a male patient diagnosed with neuroendocrine ileum. The result was an excellent localization of [¹⁴⁹Tb]Tb-DOTANOC in this neuroendocrine neoplasm, in addition to multiple lymph nodes, skeletal metastasis, and SSTR-expressing organs [170].

Currently, ¹⁴⁹Tb is predominantly produced by ISOLDE/CERN (Switzerland), TRIUMF (Vancouver, Canada), and PNPI (Gatchina, Russia) [171]. More recently, preclinical studies with [¹⁴⁹Tb]Tb-DOTANOC and [¹⁴⁹Tb]Tb-PSMA-617 were reported in tumor xenograft mouse models of AR42J pancreatic and prostate cancers, respectively [168,172]. Thus far, no clinical study has been reported with ¹⁴⁹Tb [168].

• (ii). ¹⁵²Terbium: ¹⁵²Tb is a diagnostic radionuclide that decays via positron emission (E_β⁺_avg = 1142 keV) and multiple gamma radiations, which could lead to high radiation exposure [170]. The relatively long half-life of ¹⁵²Tb (t_1/2 = 17.5 h) allows it to be useful in dosimetry estimation. In fact, ¹⁵²Tb is an exact diagnostic match for ¹⁴⁹Tb and ¹⁶¹Tb, as well as other clinically useful therapeutic radionuclides, like ¹⁷⁷Lu, due to their similarities in coordination chemistry and pharmacokinetics.

• (iii). ¹⁵⁵Terbium: ¹⁵⁵Tb is a suitable SPECT isotope, a promising alternative to the ¹¹¹In isotope, and it could be useful for dosimetry estimation of β⁻ emitters, like ¹⁷⁷Lu, ⁹⁰Y, and ¹⁶⁶Ho [173].

• (iv). ¹⁶¹Terbium: ¹⁶¹Tb decays by low-energy (E_β⁻_avg = 154 keV) (β⁻) emission, having a short tissue penetration (0.29 mm) range and a long half-life (t_1/2) of 6.8 d [174]. The decay characteristics and half-life of ¹⁶¹Tb are like ¹⁷⁷Lu (E_β⁻_avg = 134 keV, t_1/2 = 6.7d) [174], although ¹⁶¹Tb also emits a substantial number of auger electrons, which could be advantageous for therapeutic applications. However, the clinical superiority of ¹⁶¹Tb over ⁷⁷Lu is yet to be established [175,176,177]. In addition to radionuclide therapy, ¹⁶¹Tb also emits gamma photons enabling SPECT imaging [174]. Recently, Baum et al. demonstrated the first-in-human SPECT imaging using [¹⁶¹Tb]Tb-DOTATOC in patients with paraganglioma and NETs and showed high-quality images and visualization of hepatic metastasis as well as multiple osteoblastic skeletal metastasis in patients [178].

The main constraint to the wider application of Tb isotopes is their availability: there are insufficient production quantities. The production of Tb isotopes requires expensive enriched targets and accelerator-based isotope separation on-line technology (ISOLDE), which is not widely available [166]. Table 9 summarizes the clinical investigation of Tb radionuclides.

4.5.3. Production and Availability

In 2012, Muller et al. reported on the production of ^149/152/155Tb in a range of ~6–15 MBq activity through a high-energy proton-induced spallation of tantalum foil targets, followed by dissolution and isotope separation [167]. Such a high-energy proton accelerator facility and mass separation technology (ISOLDE) are limited to a few centers worldwide, including CERN, Switzerland. Lately, CERN-MEDICIS (Medical isotopes collected from ISOLDE) technology was developed, which allowed for the production of 38 GBq of ¹⁴⁹Tb, 37 GBq of ¹⁵²Tb, and 5.3 GBq of ¹⁵⁵Tb [166]. ¹⁶¹Tb (up to 15 GBq) can be produced in a neutron flux reactor using ¹⁶⁰Gd targets, as proposed by Lehenberger et al. at PSI, Switzerland [181]. Interestingly, the production concept and the cost of ¹⁶¹Tb is like a non-carrier added ¹⁷⁷Lu [181].

4.6. Radioisotopes of Zinc

4.6.1. General Information

Zinc (Zn) exists in three positron-emitting isotopes (^62/63/65Zn) that have the potential to be used as PET biomarkers of zinc trafficking in various pathological conditions [182]. Among them, ⁶²Zn has limited use because it decays to another positron-emitting isotope, ⁶²Cu (β⁺ = 98%; t_1/2 = 9.7 min), which could confound the image interpretation of PET scans [182]. Nevertheless, ⁶²Zn has been used preclinically to image zinc transport in pancreatic exocrine function [183].

Among the Zn PET isotopes, ⁶⁵Zn has the longest half-life (t_1/2 = 243.9 d), making it unsuitable for diagnostic imaging because it will cause high radiation exposure to patients over time [182]. ⁶³Zn has a favorable decay characteristic (β⁺ = 93%; t_1/2 = 38.47 min) for diagnostic imaging and pharmacokinetic studies [184,185]. The decay properties of Zn radionuclides are given in Table 10.

4.6.2. Clinical Applications

In 2016, DeGrado et al. conducted the first-in-human PET imaging study of [⁶³Zn]Zn-citrate on Alzheimer’s disease patients [185]. Although low uptake of [⁶³Zn]Zn-citrate was seen in the brain (SUV ~0.4) compared to other organs, like the liver, pancreas, kidney, and gastrointestinal tract, it was sufficient to study ⁶³Zn clearance kinetics on a regional basis in those patients. The regions with slower ⁶³Zn clearance corresponded to the regions of known amyloid-β pathology on [¹¹C]C-PiB PET scans and also the regions of lower uptake on [¹⁸F]FDG-PET scans [185]. Further imaging studies are warranted, though, to study zinc homeostasis in persons with Alzheimer’s disease.

4.6.3. Production and Availability

In 2014, DeGrado et al. developed a cyclotron-based production of ⁶³Zn via a ⁶³Cu(p,n)⁶³Zn nuclear reaction using a liquid target by irradiating an isotopically enriched solution of [⁶³Cu]Cu-nitrate [184]. ⁶³Zn was produced with a specific activity of 41.2 + 18.1 MBq/µg (uncorrected) and radionuclidic purity of 99.9% using 1.23 M of [⁶³Cu]-copper nitrate.

5. SPECT Probes

5.1. Technetium-99m

5.1.1. General Information

Technetium-99m (^99mTc) is the most widely used medical isotope in nuclear medicine, accounting for more than 80% of all nuclear medicine procedures, including myocardial perfusion imaging, cancer, and infection imaging [186]. ^99mTc-based agents are a favored choice for cardiac imaging in the U.S [187]. ^99mTc mainly disintegrates into its other isomeric ⁹⁹Tc (which is radioactive) with the release of low-energy monochromatic gamma rays (140.5 keV, 98.6%) that can be detected by any sensitive gamma cameras [188]. Despite the advent of superior PET technology and the prevalence of CT or MRI over nuclear medicine, ^99mTc-based radiopharmaceuticals have been continuously supplied in hospitals during routine clinical examinations [188]. The advantages behind them are (i) a short/sufficient half-life of 6 h, which offers minimum radiation exposure to patients, (ii) instant kit-based labeling and formulations due to rich coordination chemistry of Tc (multiple oxidation states), (iii) availability of transportable generators (⁹⁹Mo/^99mTc) for production, and (iv) cost-effective SPECT gamma cameras compared to expensive PET technology. These points have solidified the continuous application of ^99mTc-labeled radiopharmaceuticals [188,189]. The nuclear decay characteristics of ^99mTc are given in Table 11.

5.1.2. Clinical Applications

Among the various clinical applications of ^99mTc-labeled radiopharmaceuticals, [^99mTc]Tc-HYNIC-TOC (Tektrotyd) is commercially available for the imaging of metastatic NETs [190]. A recent comparative study of [⁶⁸Ga]Ga-DOATATE and [^99mTc]Tc-HYNIC-TOC (^99mTc-octreotide) on NET patients showed the superiority of [⁶⁸Ga]Ga-DOATATE over [^99mTc]Tc-HYNIC-TOC in terms of sensitivity and specificity [191]. However, it is reasonable to re-evaluate the performance of ^99mTc-radiopharmaceuticals using ultra-fast SPECT scanners that may increase the image resolution up to twofold [192]. Additionally, [^99mTc]Tc-MIP1404 and [^99mTc]Tc-MIP-1405 are the first [^99mTc]Tc-labeled PSMA ligands applied in humans [193]. Although both agents can visualize PSMA tumors and metastatic lymph node/bone lesions, [^99mTc]Tc-MIP1404 (also known as Tc-Trofolastat) is advantageous over [^99mTc]Tc-MIP-1405 to detect PCa at early stages of the disease, and it is currently registered in a phase 3 clinical trial (NCT02615067) [194]. In 2016, another promising PSMA-based SPECT agent [^99mTc]Tc-PSMA-Investigation & Surgery was applied for first-in-human radio-guided surgery (RSG) [195]. The clinical applications of ^99mTc radiopharmaceuticals are summarized in Table 12.

5.1.3. Production and Availability

^99mTc is a radioactive decay product of ⁹⁹Mo (t_1/2 = 66h), which is traditionally made in a large nuclear reactor via fission of high-enriched uranium targets (²³⁵U) [189]. The production of ^99mTc in the form of pertechnetate [^99mTc]TcO₄⁻ from the parent ⁹⁹Mo was achieved using the commercially available and transportable ⁹⁹Mo/^99mTc generators in nuclear medicine for the preparation of almost all of the ^99mTc-based radiopharmaceuticals. Until 2011, the global requirement for ⁹⁹Mo was fulfilled by seven nuclear research reactors. The mandatory shutdowns of these reactors for maintenance or due to breakdowns stopped the global supply in 2009, 2012, and 2013 [189]. To overcome such an unavoidable global crunch in the supply of ⁹⁹Mo, several research efforts were initiated, including the use of linear accelerators and cyclotrons, which utilize electron beam and proton irradiation of solid ¹⁰⁰Mo targets, respectively [205,206].

5.2. Indium-111

5.2.1. General Information

Indium-111 (¹¹¹In; t_1/2 = 2.8 d) is a SPECT isotope that decays by EC (100%) and low- energy γ-emission (171 keV, 245 keV) [207]. The decay characteristics are summarized in Table 13. Over the decades, ¹¹¹In has been used as the reference standard for SPECT-immuno imaging of Abs [207]. ^110mIn is a PET radioisotope of In with a short half-life (69 min), and it is suitable for tracking short peptides (e.g., octreotide) having faster kinetics [208].

5.2.2. Clinical Practice

In mid-1978, McAffee and Thakur introduced a radiotracer, [¹¹¹In]In-oxine, which could be used to radiolabel leukocytes (white blood cells (WBC)) for the scintigraphic detection of focal infections [209]. In 1985, the FDA approved [¹¹¹In]In-oxine-tagged WBC scans for clinical imaging of inflammatory disease [210]. The reported sensitivity and specificity of these [¹¹¹In]In-WBC scans ranged from 60–100% to 69–92%, respectively, in detecting osteomyelitis, vascular grafts infection, bone infections, etc. [211]. Other than cell labeling, ¹¹¹In was also used in radiolabeling of various peptides, proteins, Abs, and drugs. For example, [¹¹¹In]In-capromab pendetide (ProstaScint^®) was FDA-approved for immuno-SPECT imaging of PCa [212]; however, the poor tumor-to-background signals limited its routine clinical use [212]. Later, another promising PSMA immuno-SPECT tracer, [¹¹¹In]In-J591 (PSMA-Ab), was developed, and the first clinical trial was reported in 2005 [213]. Clinical trials of [¹¹¹In]In-J591 are underway and associated with the dosimetric projections of RIT with [⁹⁰Y]Y-J591 [214]. Furthermore, in 2018, Heckman et al. demonstrated the first-in-man study using the novel SPECT tracer [¹¹¹In]In-DOTA-girentuximab for intraoperative guidance of renal cell carcinoma resection in patients [215]. Table 14 summarizes the clinical application of ¹¹¹In-labeled radiopharmaceuticals.

5.2.3. Production and Availability

The most common production route of ¹¹¹In in a high yield (222 ± 5 MBq/µA.h) is proton irradiation (21 MeV) of enriched ¹¹²Cd target via a ¹¹²Cd (p,2n) ¹¹¹In nuclear reaction using a cyclotron [216]. Table 14

Clinical applications of ¹¹¹In-labeled radiopharmaceuticals.

Radiopharmaceuticals	Targets	NCT Number ^	Disease
[111In]In-CP04	CCK2R/gastrin	NCT03246659 (Phase I; completed)	Thyroid carcinoma [217]
[111In]In-Ch806	gp140, IL-13RA2	NCT00291447 (Phase I; completed)	Neoplasm [218]
[111In]In-capromab pendetide (ProstaScint®)	PSMA	NCT00992745 (Phase I; completed)	Prostate cancer [219]
[111In]In-PSMA (I&T)	PSMA	NCT04300673 (Phase I ongoing)	Prostate cancer [220]
[111In]In-DOTA-Girentuximab	Carbonic anhydrase-IX	NCT02497599 (Phase I; status unknown)	Renal cell carcinoma [221]
[111In]In-labeled leukocytes	Leukocytes	NCT00026897 (Phase II; completed)	Neoplasm [222]

NCT: National clinical trial, CCK2R/gastrin: Cholecystokinin receptor, gp140: glycoprotein 140, PSMA: Prostate-specific membrane antigen, ^ clinicaltrials.gov and data accessed on August 15, 2023.

6. Beta Minus Emitter

6.1. Yttrium-90

6.1.1. General Information

Yttrium-90 (⁹⁰Y) is a pure high-energy β⁻ emitter (E_β⁻_max = 2284 keV, E_β⁻_avg = 933 keV), which decays to stable Zr-90 with no accompanying gamma emissions [223] (Table 15). Y-90 has a longer tissue penetration depth of up to 11.8 mm [223]. To date, Y-90 has been radiolabeled with tumor-targeting Abs [224], SSTR-targeting peptides [225], and resins/glass microspheres to treat a variety of tumors [226].

6.1.2. Clinical Application of ⁹⁰Y

The FDA has approved two types of ⁹⁰Y microspheres, TheraSphere^TM (glass microspheres) and SIR-spheres^® (resin microspheres), to treat unresectable hepatocellular carcinoma and colorectal metastasis, respectively [227,228].

These ⁹⁰Y-microspheres have been used in therapies based on the concept of “radioembolization” (also known as selective internal radiation therapy); it is a promising catheter-based liver-directed therapy approved by the FDA for patients with primary/metastatic liver tumors. It was found that the antitumor effect of ⁹⁰Y-microspheres (glass microspheres, also known as Thersphere^TM) are related to beta radiations rather than embolization and therefore proven safer/successful for advanced-stage liver cancer [227]. The recent phase III trials of radioembolization of ⁹⁰Y-resin microspheres in patients with HCC demonstrated significantly higher tumor response with respect to standard first-line treatment with Sorafenib. However, these results did not meet the primary endpoint, such as overall survival or the patient’s quality of life. Several Asian guidelines recommend ⁹⁰Y-resin microspheres for HCC treatment based on certain considerations, such as patient selection, treatment planning using accurate dosimetry pre/post-radioembolization, and technical aspects [229,230,231,232].

In 2002, the FDA approved the first anti-CD20 radioimmunoconjugate [⁹⁰Y]Y-Ibritumomab tiuxetan (Zevalin^TM) for the treatment of advanced B-cell lymphoma as a first line of treatment for rituximab-relapsed or refractory low-grade lymphomas; the overall response rate has ranged from 74% to 82% [233]. Despite the demonstrated immunotherapy efficacy of Zevalin^TM, it failed commercially due to the underutilized practice by hematologist–oncologists for logistic and economic reasons [234]. In addition, other competitive RIT drugs, such as rituximab (anti-CD20) and second-generation mAbs, undoubtedly contributed to the limited sale of Zevalin^TM [235,236].

The development of second-generation mAbs, particularly bispecific Abs (e.g., biotin, IgG-single chain variable fragment), have been utilized in an alternative approach called multi-step pre-targeted RIT to enhance the therapeutic efficacy and to diminish its toxicities [237]. Based on PRIT technology, [⁹⁰Y]Y-DOTA-biotin was developed, which makes a strong conjugate with Abs (streptavidin, avidin) present on the tumor. In 1999, Paganelli et al. published the first clinical preliminary results of [⁹⁰Y]Y-DOTA-biotin for the treatment of high-grade gliomas (n = 48) based on biotin–streptavidin chemistry and showed tumor reduction (>25–100%) in 25% of patients; in 16% of these, the response lasted for at least a year [238]. In 2000, a phase II clinical trial of [⁹⁰Y]Y-DOTA-biotin was reported in patients with metastatic colon cancer [239]. Despite evaluating the feasibility, safety, and efficacy of [⁹⁰Y]Y-DOTA-biotin, the immunogenicity of these types of pre-targeting agents have not been addressed, which in turn caused the clinical trials to end in 2005 [240].

Paganelli et al. developed an innovative therapeutic approach called “Intra-operative avidination for radionuclide therapy” (IART^®) that relies on a biotin–avidin binding system [241]. A phase II study of IART^® in 2010 using [⁹⁰Y]Y-DOTA-biotin on breast cancer patients demonstrated its potential use immediately after breast resection, thereby shortening the time course of external beam radiotherapy [241]. In the past decade, several peptide-based ⁹⁰Y-tracers were developed for PRRT, and they are currently under clinical trial. Table 16 summarizes the clinical application of ⁹⁰Y radiopharmaceuticals.

6.1.3. Production and Availability

⁹⁰Y can be produced from the ⁹⁰Sr/⁹⁰Y generator, where the parent isotope is ⁹⁰Sr (t_1/2 = 29 y), and it can be generated as a by-product in large quantities in U-based nuclear reactions [242]. Commercial availability and the steady supply of Y-90 are advantageous in conducting Y-90-based clinical trials.

Table 16

Clinical applications of ⁹⁰Y-labeled radiopharmaceuticals.

Radiopharmaceuticals	Target	NCT Number ^	Disease
[90Y]Y-cG250	-	NCT00199875 (Phase I; completed)	Renal and kidney cancer [243]
[90Y]Y-hM5A	CEA	NCT00645060 (Phase I; completed) NCT01205022 (Phase I; completed)	Unspecified adult solid tumor [244]Colon and rectal cancer [245]
[90Y]Y-hPAM4	MUC1	NCT00603863 (Phase I/II; completed)	Pancreatic [246]
[90Y]Y-DOTATOC	SSTR	NCT05568017 (Phase II; ongoing)	Pancreatic neuroendocrine tumor [247]
[90Y]Y-edotreotide	SSTR	NCT00006368 (Phase I; completed)	Brain, breast, and lung cancer, lymphoma, melanoma, neoplastic syndrome [248]
[90Y]Y-resin microspheres (SIR-spheres®)	-	NCT01482442 (Phase III; completed)	Liver carcinoma [249]
[90Y]Y- Ibritumomab Tiuxetan (ZevalinTM)	CD20 + B cells	NCT01446562 (Phase II; completed)	Follicular lymphoma [250]

NCT: National clinical trial, CEA: Carcinoembryonic antigen, MUC1: Mucin 1, SSTR: Somatostatin receptors, CD20: Cluster of differentiation 20, ^ clinicaltrials.gov and data accessed on 15 August 2023.

6.2. Radioisotopes of Rhenium

6.2.1. General Information

Among several radioisotopes of rhenium (Re), ¹⁸⁶Re and ¹⁸⁸Re are recognized for their therapeutic potential, and they were used to develop various therapeutic radiopharmaceuticals. In addition to beta emission,¹⁸⁶Re and ¹⁸⁸Re also emit low-abundant γ-rays of 137 keV and 155 keV, respectively (Table 17), that permit scintigraphic monitoring and dosimetry calculations via SPECT imaging [251].

Given the two distinct tissue penetration ranges of ¹⁸⁸Re (11 mm) and ¹⁸⁶Re (4.5 mm), they can be selectively applied for treating large-sized tumors and small- or mid-sized tumors, respectively [251]. Moreover, to better understand the biodistribution, ^99mTc represents a diagnostic match for ^186/188Re radioisotopes, as both Re and Tc exhibit similar chemical properties [251]. However, ^99mTc- and ¹⁸⁸Re-labeled radiotracers do not always show the same in vivo biodistribution [251].

6.2.2. Clinical Applications of Rhenium Radioisotopes

¹⁸⁸Re-labeled therapeutic radiopharmaceuticals have been investigated in multiple clinical trials involving primary tumors, bone metastasis, rheumatoid arthritis, and intracoronary β-brachytherapy [252]. In 1998, Maxon et al. evaluated phosphonate-based radiotracer [¹⁸⁸Re]Re-HEDP for bone pain palliation [253]. Bone pain is a major issue in ~50% of women with breast cancer and 80% of men with PCa. A phase III trial comparing [¹⁸⁸Re]Re-HEDP with a well-known bone-targeting agent [²²³Ra]RaCl₂ is ongoing (NCT03458559). The primary objective of this study is to compare the overall survival in patients with PCa metastatic to bone after treatment with [¹⁸⁸Re]Re-HEDP and [²²³Ra]RaCl₂. Several Ab fragments have been radiolabeled with ^186/188Re for RIT. These include alemtuzumab (anti-CD66) in leukemia [254], rituximab (anti-CD20) in lymphoma [255], MN-14 (ant-CEA) in gastrointestinal cancer [256], and bivatuzumab in head and neck cancers [257]. Among them, the evaluation of [¹⁸⁶Re]Re-bivatuzumab in a variety of diseases (NCT02204033) as a phase I clinical trial has been completed. However, the results are not yet published. Recently, ¹⁸⁸Re-colloids-based brachytherapy kit (Rhenium-SCT^®) became commercially available to treat basal cell carcinoma or squamous cell carcinoma, particularly to the face and neck, where surgery and radiotherapy are either not possible or refused by patients (NCT05135052) [258]. Several preliminary clinical reports have demonstrated that this innovative epidermal therapy is effective in 98% of melanoma patients even after a single application [259]. The clinical investigations of Re radiopharmaceuticals are summarized in Table 18.

6.2.3. Production and Availability

¹⁸⁸Re is routinely produced in high specific activity by a ¹⁸⁸W/¹⁸⁸Re generator, like the ^99mTc generator [260]. On other hand, ¹⁸⁶Re is most commonly produced in apparent specific activity of 111–148 GBq/mg at the Missouri Research Nuclear Reactor [261].

Table 18

Clinical applications of ¹⁸⁸Re-labeled radiopharmaceuticals.

Radiopharmaceuticals	Targets	NCT Number ^	Disease
[188Re]Re-HEDP vs. [223Ra]RaCl2	Bone metastasis	NCT03458559 (Phase III; ongoing)	Prostate cancer metastatic to bone [262]
[186Re]Re-labeled bivatuzumab	VEGF-A	NCT02204046 (Phase I; completed), NCT02204059 (Phase I; completed), NCT02204033 (Phase I; completed)	Adenocarcinoma [263]Non-small cell lung carcinoma [264]Head and neck neoplasm [265]
Rhenium-SCT®	Skin lesions	NCT05135052 (Phase not applicable; ongoing)	Non-melanoma skin cancer [258]
[186Re]Re-nanoliposome	-	NCT01906385 (Phase I/II; ongoing)	Glioma [266]

NCT: National clinical trial, VEGF-A: Vascular Endothelial Growth Factor Receptors-A, ^ clinicaltrials.gov and data accessed on 15 August 2023.

6.3. Holomium-166

¹⁶⁶Ho is not only a β⁻ emitter but also a gamma emitter; it is one of the lanthanide radionuclides that can be imaged using SPECT and MRI [267,268]. ¹⁶⁶Ho is a theranostic radionuclide with favorable physical decay characteristics, including a sufficient half-life of 26.6 h, an average emission energy of (E_βav) of 670 keV, a soft tissue penetration range of 8.7 mm, and a low-energy γ-emission (80.5 keV, 6%) for SPECT imaging [267,268]. Being a lanthanide with its paramagnetic properties, ¹⁶⁶Ho-labeled drugs enable the visualization and quantification of the biodistribution of drugs in the tumor tissues by means of SPECT and MRI [268]. In 1991, Murphy et al. first investigated the potential possibility of ¹⁶⁶Ho microspheres for the internal radiation therapy of hepatic tumors in rabbits [269]. In 2010, Smith et al. investigated the first ¹⁶⁶Ho-based liver radioembolization, which was followed by growing interest in this treatment possibility, as evidenced by the increasing number of publications in the last few years [268]. In terms of clinical applications, ¹⁶⁶Ho-microspheres serve as an alternative to existing ⁹⁰Y microspheres to treat liver tumors, with potential advantages of the shorter half-life of ¹⁶⁶Ho (t_1/2 = 26.6 h) compared to ⁹⁰Y (t_1/2 = 64 h) along with its quantification by MRI [268]. Additional information on ¹⁶⁶Ho- radiopharmaceuticals have been discussed in a recent review by Klaassen et al. [270]; therefore, we have kept the discussion extremely short.

6.4. Lutetium-177

6.4.1. General Information

¹⁷⁷Lu is currently the most important and highly valuable theranostic β⁻/γ-emitting radionuclide in nuclear medicine across the globe [271]. ¹⁷⁷Lu has a long half-life (t_1/2 = 6.7d) and decays to ¹⁷⁷Hf by emitting medium-energy cytotoxic β⁻ particles, with the most abundant β⁻ particles (78%) having a maximum energy of 0.497 MeV (Table 19) [271]. Furthermore, the co-emission of γ-photons (112.9 keV, 208.5 keV) enables the visualization and quantification (dosimetry) of the biodistribution of ¹⁷⁷Lu- radiopharmaceuticals using SPECT [271].

6.4.2. Clinical Applications

Since 2000, ¹⁷⁷Lu-labeled somatostatin analogues have been utilized in PRRT for the treatment of inoperable or metastatic NETs [272]. ¹⁷⁷Lu-labeled somatostatin has six- to seven-fold higher affinity for SSTR2 compared with its ⁹⁰Y-loaded counterpart [272]. Several preclinical and clinical studies have been conducted on the therapeutic effectiveness of ¹⁷⁷Lu-based radiopharmaceuticals in last two decades [273]. In 2005, the first-in-human proof-of-concept study was published on endoradiotherapy with [¹⁷⁷Lu]Lu-PSMA-I & T, which was found to be promising in patients with castration-resistant and metastatic prostate cancers [274].

In 2017, the results of a clinical phase 3 trial (NETTER-1) involving 229 patients randomized to either PRRT using [¹⁷⁷Lu]Lu-DOTATATE (7.4 GBq every 8 weeks) or a long-acting release (LAR) formulation of octreotide (control groups) to treat patients with midgut NET were released [275,276]. The groups receiving [¹⁷⁷Lu]Lu-DOTATATE had a significantly higher response rate (18%) and longer progression-free survival (65.2%) at 20 months compared to the controls, with 10.8 and 3%, respectively. [¹⁷⁷Lu]Lu-DOTATATE treatment yielded a clinically significant improvement in progression-free survival as a primary end point as well as an improvement in the median survival of 11.7 months [276]. Overall, the treatment was well tolerated with grade 3 or 4 adverse events, which were similar in both the groups. No evidence of renal toxicities was observed among patients in the [¹⁷⁷Lu]Lu-DOTATATE groups [275,276]. In 2018, the U.S. Food and Drug Administration (FDA) and the European Medicines Agency (EMA) approved [¹⁷⁷Lu]Lu-DOTATATE (Lutathera ^®; Novartis company) for mid gut NET. In the same year, [¹⁷⁷Lu]Lu-PSMA-617 was proposed for the treatment of metastatic castration-resistant prostate cancer (mCRPC). The results of a phase 2 trial (TheraP) demonstrated a significant decline (>50%) in PSA in the groups treated with [¹⁷⁷Lu]Lu-PSMA-617 compared to the standard treatment using Carbazitaxel, which eventually led to FDA and EMA approvals under the name of Pluvicto^®(Novartis). The clinical use of ¹⁷⁷Lu-radiopharmaceuticals has been increased in the past few years, including [¹⁷⁷Lu]Lu-FAPI-46 [277,278] and combination therapy with ⁹⁰Y-labeled peptides and chemotherapy. Several clinical trials of ¹⁷⁷Lu-based radio theranostics are underway, as listed in Table 20.

6.4.3. Production and Availability

There are two common independent ways to produce large-scale ¹⁷⁷Lu in nuclear reactors. The first is a direct production route (also known as carrier added or c.a.) based on neutron irradiation of ¹⁷⁶Lu via ¹⁷⁶Lu (n, γ) ¹⁷⁷Lu nuclear reaction in medium–high-energy reactors [271,288]. The second approach is an indirect production route (also known as non-carrier added or n.c.a) based on neutron irradiation of ¹⁷⁶Yb target via ¹⁷⁶Yb (n, γ) ¹⁷⁷Yb →¹⁷⁷Lu in high-energy flux reactors [288]. The advantage of the direct production route is that it can create large quantities of ¹⁷⁷Lu (740–1110 GBq) using ¹⁷⁶Lu; however, the major concern is the co-emission of small amounts of long-lived radioactive impurity of ^177mLu along with “useful” ¹⁷⁷Lu. Additionally, only a part of the target matrix (or carrier) of ¹⁷⁶Lu is converted into the desired ¹⁷⁷Lu, which cannot be chemically isolated as they are the isotopes of the same element; this therefore decreases its specific activity [288].

On the other hand, the indirect approach using highly enriched ¹⁷⁶Yb (>98%) produces high specific activity (>2.96 TBq/mg) non-carrier-added ¹⁷⁷Lu; however, this process requires a suitable method for the radiochemical separation of ¹⁷⁷Lu from ¹⁷⁶Yb, which is quite challenging, especially in large-scale or industrial settings, to meet the surging demand [271,288]. ¹⁷⁷Lu can also be produced in cyclotron using deuteron beams (<6 MeV); however, this is less explored due to the low production yield [288].

7. Alpha-Particle-Emitting Radiopharmaceuticals

Alpha radiations are better suited for the treatment of small metastasis due to their short tissue penetration range and high LET per micrometer of tissue compared to β⁻-emitting radionuclide via double strand DNA breaks in cancerous cells, while sparing nearby healthy tissues [21]. However, due to the early stages of development of alpha-targeted radionuclide therapy, most clinical trials continue to use beta-emitting radionuclide therapy rather than alpha-emitting radionuclide therapy.

7.1. Radioisotopes of Bismuth

7.1.1. General Information

Two promising medically relevant isotopes of bismuth (Bi) with mixed α/β⁻ emission properties are ²¹²Bi and ²¹³Bi [289]. ²¹²Bi undergoes β⁻ decay (64%) to ²¹²Po (α-emitter) and α-decay (36%) to ²⁰⁸Tl (β⁻ emitter). Both daughters of ²¹²Bi (²¹²Po and ²⁰⁸Tl) further decay to the stable ²⁰⁸Pb [289]. Moreover, the emission of high-energy γ-rays through the decay of ²⁰⁸Tl (2.6 MeV) necessitates appropriate shielding to avoid radiation exposure, making it a less favorable choice over ²¹³Bi [289].

²¹³Bi is considered a magic bullet in targeted radionuclide therapy. The isotope predominantly undergoes β⁻ decay (97.8%) to the pure α-emitter ²¹³Po, whereas the remaining ²¹³Bi (2.2%) undergoes α- decay to beta-emitter ²⁰⁹Tl [289]. Both of these daughter nuclei (²¹³Po, ²⁰⁹Tl) decay to ²⁰⁹Pb, which further decays to long-lived ²⁰⁹Bi (essentially stable) [289]. In addition, ²¹³Bi also emits γ-radiation (440 keV) that can be employed for SPECT imaging [289]. Overall, each ²¹³Bi decay delivers only one α particle (5.9–8.4 MeV) [289]. The nuclear decay properties of Bi radionuclides are given in Table 21.

7.1.2. Clinical Applications of ²¹³Bismuth

In 2002, Joseph et al. reported the first proof-of-concept phase I study demonstrating the anti-leukemic effect of ²¹³Bi conjugated with anti-leukemia Ab HuM195 (Lintuzumab) to treat leukemia patients [290]. In a subsequent clinical study (phase I/II) in 2010, complete remission was seen in acute myeloid leukemia patients with sequential administration of [²¹³Bi]Bi-HuM195 (37 MBq/Kg) and the chemotherapy drug cytarabine (Table 22) [291]. The promising clinical results with [²¹²Bi]Bi-mAb-TAT initiated its use to treat other cancers, including melanoma, NETs, and glioma [292,293].

In the last two decades, research efforts have facilitated the development of ²¹³Bi-based peptide conjugates for PRRT study. In 2014, Kratochwil et al. reported the first and only radiopeptide therapy with [²¹³Bi]Bi-DOTATOC on NET patients, which was refractory to β⁻ therapy with ⁹⁰Y/¹⁷⁷Lu-DOTATOC [294]. The results indicated that TAT could induce considerable and long-lasting remission in both the primary tumor and liver metastases [294]. Another tracer, [²¹³Bi]Bi-PSMA-617, was reported in metastatic castration-resistant PCa patients [295]. The remarkable drop in prostate-specific antigen levels from 237 µg/L to 43 µg/L after [²¹³Bi]Bi-PSMA-617 treatment showed the great potential of TAT using [²¹³Bi]Bi-PSMA-617 over conventional β⁻ radionuclide therapy. In addition, TAT may be able to break the radioresistant effect of β⁻ emitters [295]. In the past few years, different clinical trials have used ²¹³Bi-carrying radiopharmaceuticals for the treatment of various diseases. Although the outcomes were encouraging, further investigations are needed to ensure its safety and efficacy in the clinic.

Table 21

Decay characteristics of radioisotopes of Bismuth ^#.

Isotope	Half-Life (t1/2)	Decay Characteristics	Parent Nuclides and Their Daughter Nuclides	Energies (MeV)		Eγ; keV (Intensity%)
				Eα (MeV)	Eβ− (MeV)
212Bi	61 min	β− = 64%α = 36%	212Bi212Po208Tl208Pb (stable)	212Bi-6.1212Po-8.8	212Po-0.769208Tl-0.557	212Bi-727.3 (6.6)208Tl-277.4 (6.3), 510.8 (22.6), 583.2 (84.5), 763 (1.8), 860.6 (12.4), 2614.5 (99.2)
213Bi	45.6 min	β− = 98%α = 2%	213Bi213Po209Tl209Pb209Bi (essentially stable)	5.9 (213Bi)8.4 (213Po)	213Bi-1400209Tl-2000209Pb-600	213Bi-440 (25.9)

^# Data on ²¹²Bi are from [296] and data on ²¹³Bi are from [297]. Please refer to Scheme 2.

7.1.3. Production and Availability

Many α-emitter radionuclides are produced from naturally occurring heavy α radionuclides, including U, radium (Ra), and actinium (Ac). The clinical amount of ²¹³Bi is obtained from its parent radionuclide ²²⁵Ac (t_1/2 = 9.9 d) as a ²²⁵Ac/²¹³Bi generator [298]. The parent isotope ²²⁵Ac is obtained from the decay of ²²⁹Th (t_1/2 = 7317 y), which in turn originates from a decay chain of fissile materials of ²³³U [298]. The relatively long half-life of the parent radionuclide ²²⁵Ac allows shipment of the ²²⁵Ac/²¹³Bi generator to any radiopharmaceutical facility located even long distances away and permits in-house generation of ²¹³Bi for radiolabeling purposes over weeks to months. However, the limited global production of ²²⁹Th and the concern for the non-proliferation of the fissile product of ²³³U restricted the commercial supply of ²²⁵Ac stocks to produce ²¹³Bi-labeled radiopharmaceuticals [293]. An alternate route to producing ²²⁵Ac is using proton irradiation of ²²⁶Ra targets via ²²⁶Ra (p,2n) ²²⁵Ac in a cyclotron; still, the presence of hazardous ²²²Rn poses serious limitations in clinical translation and waste disposal [293].

Table 22

Clinical applications of ²¹³Bi-labeled radiopharmaceuticals.

Radiopharmaceuticals	Targets	NCT Number ^	Disease
[213Bi]Bi-M195	CD33	NCT00014495 (Phase I/II completed)	Leukemia, myelodysplastic syndromes [299]

NCT: National clinical trial, ^ clinicaltrials.gov and data accessed on 15 August 2023.

7.2. Actinium-225

7.2.1. General Information

²²⁵Ac is one of the promising therapeutic isotopes for α-RIT of cancer. It decays to six principal intermediate radionuclide progenies (²²¹Fr, ²¹⁷At, ²¹³Bi, ²¹³Po, ²⁰⁹Tl, ²⁰⁹Pb) before reaching the stable ²⁰⁹Bi [300]. Overall, ²²⁵Ac decay (t_1/2 = 9.9 d) contributes to the emission of four α-, three β⁻, and two principal γ-emissions (218 keV; ²²¹Fr, 440 keV; ²¹³Bi), from which recognizable ²²⁵Ac results as a “nanogenerator.” ²²⁵Ac is also considered an in vivo generator of ²¹³Bi and an alternative to ²¹³Bi-based TAT, presumably because of the four α- emissions and its longer half-life compared to ²¹³Bi (t_1/2 = 45.6 min) [300]. The decay characteristics of Bi radionuclides are given in Table 23.

²²⁵Ac-based radiopharmaceuticals are prone to the redistribution of daughter progenies, particularly ²¹³Bi, which can induce renal toxicity and dose-limiting toxicity to other organs [300]. Moreover, dosimetry is essential using an isotope with a similar half-life and chelation chemistry to ²²⁵Ac (e.g., Ln³⁺) to track the biodistribution of ²²⁵Ac accurately. A handful of clinical trials of ²²⁵Ac are underway.

7.2.2. Clinical Applications of Actinium-225

In 2011, the first clinical study of α therapy was reported showing the anti-leukemic effect of [²²⁵Ac]-lintuzumab in acute myeloid leukemia patients (>60 y) [301]. Motivated by the initial findings, several clinical trials (phase I/II), including a dose-escalation study of [²²⁵Ac]Ac-lintuzumab combined with low-dose chemotherapeutic drugs (e.g., mitoxantrone, cladribine), have been initiated (NCT03867682 [258]).

By 2018, a multicenter phase I study using [²²⁵Ac]Ac-FPI-1434 (NCT03746431) was designed to treat solid tumors from non-cell lung, prostate, and breast carcinomas [302]. Recently, a clinical study reported by Kratochwil et al. demonstrated the remarkable anti-tumor effect of [²²⁵Ac]Ac-PSMA-617 (100 kBq/kg) in 81% of metastatic castration-resistant PCa patients [303]. Additional clinical trials are warranted to further investigate the anti-tumor potential of [²²⁵Ac]Ac-PSMA-617 TAT in men with prostate cancer (NCT04597411). The clinical investigation of ²²⁵Ac radiopharmaceuticals is summarized in Table 24.

7.2.3. Production and Availability

Currently, the clinical supply of ²²⁵Ac is produced from ²²⁹Th generators (t_1/2 = 7340 y), which are obtained from the parent ²³³U (t_1/2 = 160,000y) [300]. ²²⁹Th generators are available at the Oak Ridge National Laboratory USA, the Institute of Transuranium Elements, Germany, and the Institute of Physics and Power, Russia [300]. However, as of 2008, the approximate total worldwide production of ²²⁵Ac accounts for only 68 GBq/year, which can support only several hundred patients per year. Therefore, large-scale production of ²²⁵Ac is needed. Alternative production routes are being explored, including proton irradiation of ²²⁶Ra targets, which could produce sufficient quantities of ²²⁵Ac due to the relatively high reaction cross-section; however, the handling of ²²⁶Ra (t_1/2 = 1600 y) is challenging [300].

To date, the accelerator-based production route involves high-energy proton irradiation (>100 MeV) of natural thorium (²³²Th), and it could serve as another potential path for the future production of ²²⁵Ac. This method may yield twenty times greater quantities of ²²⁵Ac than the current annual production worldwide [310].

7.3. Radioisotopes of Lead

7.3.1. General Information

Lead (²¹²Pb; t_1/2 = 10.6 h) is a β⁻-emitting radionuclide that decays to ²¹²Bi (t_1/2 = 61 min), which decays by mixed α/β- particle emission [311]. Importantly, ²¹²Pb also emits imageable γ-radiation (238.6 keV) that has the potential to image ²¹²Pb-labeled radiopharmaceuticals directly via SPECT imaging (Table 25). Moreover, ²⁰³Pb is a γ-emitting analogue of ²¹²Pb, and it is considered an ideal SPECT imaging isotope for the estimation of an accurate dosimetry for ²¹²Pb-labeled therapeutic radiopharmaceuticals [311].

7.3.2. Clinical Practice

During 2014–2018, Meredith et al. performed several clinical studies using therapeutic [²¹²Pb]Pb-TCMC-trastuzumab in HER2 expressing malignancy, with promising outcomes, including improved safety, tolerability, and therapeutic efficacy [313,314,315]. Delpassand et al. investigated [²¹²Pb]Pb-DOTAMTATE (Alpha Medix^TM) for the treatment of inoperable SSTR-NETs, which could be superior to the gold standard β⁻-emitting [¹⁷⁷Lu]Lu-DOTATATE radiopharmaceutical [316]. The clinical investigation of Pb radiopharmaceuticals has been detailed in Table 26.

7.3.3. Production and Availability

²¹²Pb is commonly produced from the decay chain of a ²²⁸Th (t_1/2 = 1.9 y) generator, followed by its elution in 2M HCl using a cation exchange column with a maximum yield of 85% [319]. At high radioactivity (>37 MBq), however, the radiolytic damage of the cation exchange resin in the ²²⁸Th generator increases the back pressure and decreases the yield [319]. To circumvent this, an alternative generator using ²²⁴Ra (t_1/2 = 3.7 d) was designed, which serves as a source of either ²¹²Bi or its parent nuclide ²¹²Pb [319]. The ²²⁴Ra/²¹²Pb generator could elute ²¹²Pb with a radioactivity up to ~600 MBq (16 mCi) [319]. Currently, ²¹²Pb is mainly supplied by OranoMed and Oak Ridge National Laboratory [319]. McNeil et al. established a production protocol of ²⁰³Pb via proton irradiation of either natural thallium (Tl) or enriched ²⁰³Tl in a TR13 (13 MeV) cyclotron to create a ^228Th/²¹²Pb generator for ²¹²Pb [312].

7.4. Radioisotopes of Radium

7.4.1. General Information

Ra has several radioisotopes, of which ²²³Ra and ²²⁴Ra are of considerable interest to the medical field as bone-seeking α-emitters for TAT [320]. ²²³Ra (t_1/2 = 11.4 d) is an α-emitter that decays to ²⁰⁷Pb via six intermediate progenies (²¹⁹Rn, ²¹⁵Po,²¹¹Pb, ²¹¹Bi, ²¹¹Po, ²⁰⁷Tl) and delivers four α particles and two beta particles (Table 27) [320]. However, ²²³Ra faces challenges in quantitative imaging because of the limited abundance of short-range gamma photons (<2%) [321]. Several research studies are ongoing to investigate its dosimetry approach [322,323,324].

²²⁴Ra is a pure α-emitter that decays via a series of six daughter nuclides (²²⁰Rn, ²¹⁶Po,²¹²Pb, ²¹²Bi, ²¹²Po, ²⁰⁸Tl) and emit overall four alpha particles and two beta particles before stabilizing to ²⁰⁸Pb [325]. ²²⁴Ra emits abundant gamma emissions at 241 keV that can be employed for SPECT imaging [325]. ²²⁴Ra (3.6d) has a shorter half-life than ²²³Ra (11.4d), but its decay profile and biokinetics are like ²²³Ra [307].

Table 27

Decay characteristics of radioisotopes of Radium ^#.

Isotope	Half-Life (t1/2)	Decay Characteristics	Parent and Daughter Nuclides	Energy (MeV)		Eγ; keV (Intensity%)
				Eα max	Eβ-max
223Ra	11.4 d	α = 100%	223Ra219Rn215Po211Pb211Bi211Po207Tl207Pb (stable)	223Ra-5.78219Rn-6.88215Po-7.53211Bi-6.68211Po-7.59	211Pb-0.45211Bi-0.01207Tl-0.49	144.27 (3.36)154.2 (5.84)323.8 (4.06)328.2 (2.85)
224Ra	3.6d	α = 100%	224Ra220Rn216Po212Pb212Bi212Po208Tl208Pb (stable)	224Ra-5.7220Rn-6.3216Po-6.8212Bi-6.1212Po-8.8	212Pb-0.1212Bi-0.8208Tl-0.6	241(4.1%)

^# Data on ²²³Ra are from [326,327], and data on ²²⁴Ra are from [328]. Please refer to Scheme 2.

7.4.2. Clinical Practice

From mid-1940 to 1990, [²²⁴Ra]RaCl₂ of high doses (up to 140 MBq) was used to treat different bone and joint diseases, mainly in Germany, but this practice was abandoned for technical and commercial reasons [329]. During 2000–2005, the use of [²²⁴Ra]RaCl₂ (low dose up to 10 MBq) was revived to treat ankylosing spondylitis patients, but this was discontinued in 2005 due to the enhanced risk of malignant disease following injection [330,331]. One of the potential drawbacks of ²²⁴Ra is the release of progeny β⁻-emitting ²¹²Pb with a significant half-life of 10.6 h, which could cause unwanted non-target exposure [330]. Therefore, alternative delivery strategies are of considerable interest, which could promote the retention of the daughter nuclides or mitigate their recoiling spread.

During 2007–2015, several preclinical studies investigated brachytherapy using ²²⁴Ra-loaded diffusing α-emitter radiation therapy (DaRT) wires or seeds, which minimizes the damage to surrounding normal tissues [332]. The first-in-human clinical study based on DaRT was reported in 2020 and involved the implantation of ²²⁴Ra seeds to treat squamous cancers of the skin and head [333]. Complete response to the ²²⁴Ra-DaRT treatment was observed in 22 of the 28 patients; the remaining 6 patients showed only a partial response (>30% tumor reduction) [333]. Like ²²⁴Ra, ²²³Ra was also studied for the treatment of bone skeletal metastasis. The first clinical study (phase I) in prostate and breast cancer patients was reported by Nilsson et al. in 2005 [334]. Later, the favorable clinical results (phase II/III) of [²²³Ra]RaCl₂ to treat metastatic PCa led to FDA approval of [²²³Ra]RaCl₂ (Xofigo^®; Bayer) in 2013 [335,336]. Several clinical trials of ²²³Ra-based radionuclide therapy in combination with chemotherapy (docetaxel, paclitaxel), hormonal therapy (abiraterone, enzalutamide), and immunotherapy are ongoing (Table 28).

7.4.3. Production and Availability

²²³Ra is mainly produced from ²²⁷Ac/²²⁷Th generators, where ²²³Ra is separated from ²²⁷Ac/²²⁷Th mother radionuclides using separation columns [300,350]. On the other hand, ²²⁴Ra is usually produced from a ²²⁸Th generator, where ²²⁸Th is immobilized on actinide resin, which allows regular elution of ²²⁴Ra in 1M HCl [351].

7.5. Thorium-227

7.5.1. General Information

²²⁷Th (a progenitor of ²²³Ra) is an α-emitting radionuclide that decays to ²²³Ra, which further decays by a series of α and β⁻ emissions before stabilizing to ²⁰⁷Pb [352] (Table 29). ²²⁷Th can be readily chelated with 3, 2-hydroxypyridone-N-oxide (HOPO). When ²²⁷Th is conjugated with tumor-targeting moieties, they are collectively called targeted thorium-227 conjugates (TTCs) [353].

7.5.2. Clinical Practice

There are four clinical trials listed for ²²⁷Th-based TTCs registered in the US National Library of Medicine. These trials are based on ²²⁷Th-labeled anti-PSMA-HOPO (Bay 2315497) and ²²⁷Th-labeled anti-mesothelin-HOPO for the treatment of PCa (NCT03724747) [355] and mesothelioma (Bay 2287411), respectively. The remaining two trials are based on ²²⁷Th-labeled epratuzumab-HOPO (Bay 1862864) and ²²⁷Th-labeled trastuzumab-HOPO (Bay 2701439) to treat CD22-positive non-Hodgkin’s lymphoma and HER2-positive breast or gastric cancers, respectively (Table 30). ⁸⁹Zr-labeled HOPO has the potential to serve as a PET surrogate for TTCs, which could support the clinical development of novel TTCs by providing crucial pharmacokinetic and pharmacodynamic information [356].

7.5.3. Production and Availability

²²⁷Th is produced as a decay product of the parent β⁻ emitter ²²⁷Ac (t_1/2 = 21.8 year) [357]. The longer half-life of ²²⁷Th (t_1/2 = 18.7 days) allows for the shipment of cGMP-grade ²²⁷Th solution worldwide [357].

Table 30

Clinical applications of ²²⁷Th-labeled radiopharmaceuticals.

Radiopharmaceuticals	Targets	NCT Number ^	Disease
[227Th]Th-anti PSMA (BAY2315497)	PSMA	NCT03724747 (Phase I; ongoing)	Metastatic castration-resistant prostate cancer [355]
[227Th]Th-anti Mesothelin (BAY2287411)	Mesothelin	NCT03507452 (Phase I; completed)	Advanced recurrent serous ovarian, malignant peritoneal mesothelioma, pancreatic adenocarcinoma [358]
[227Th]Th-trastuzumab (BAY2701439)	HER2+	NCT04147819 (Phase I; ongoing)	Cancer with HER2 + expression [359]
[227Th]Th-epratuzumab (BAY1862864)	CD22	NCT02581878 (Phase I; completed)	Non-Hodgkin lymphoma [360]

NCT: National clinical trial, PSMA: Prostate-specific membrane antigen, HER2+: Human epidermal growth factor 2, CD22: Cluster of differentiation 22, ^ clinicaltrials.gov and data accessed on 15 August 2023.

7.6. Radioisotopes of Astatine

7.6.1. General Information

Astatine-221 (²¹¹At) is an α-emitting therapeutic radionuclide that decays into two branches either by α-emission (42%) to ²⁰⁷Bi (t_1/2 = 33.9 y) or by EC (58%) to ²¹¹Po (t_1/2 = 516 ms); both eventually decay to a stable ²⁰⁷Pb [361]. Each decay yields one α particle and the emission of characteristic X-rays (70–90 keV) through the decay of ²¹¹Po and could be used for SPECT imaging and quantification of ²¹¹At [361]. ²⁰⁹At (t_1/2 = 5.4 h) is another isotope that predominantly decays by β⁺ emission (96%) and has been introduced as a theranostic pair to ²¹¹At (Table 31) [361]. ²¹¹At is a more attractive radionuclide than other α-emitting radionuclides because of its suitable half-life of 7.2 h, the absence of long-lived and/or toxic progenies, and its feasibility to be produced in decent quantities [361].

7.6.2. Clinical Practice

Although ²¹¹At-labeled TAT agents were discovered more than 30 years ago, only a few clinical studies using ²¹¹At-labeled Abs have been published. Zalutsky et al. reported on the application of ²¹¹At-labeled chimeric anti-tenascin mAb 81C6 (71–347 MBq) in recurrent brain tumor patients with an encouraging median survival time of 52 weeks compared to 23 weeks reported for recurrent glioblastoma multiforme patients treated with best care [362]. Another clinical study of intraperitoneal α particle therapy was reported using [²¹¹At]At-MX35(Fab) in relapsed ovarian cancer patients [363]. The results showed that there was no apparent radiation-induced toxicity discovered in patients for up to 12 years and no decreased tolerance to relapse therapy. The clinical investigation of ²¹¹At-labeled radiopharmaceuticals is summarized in Table 32.

7.6.3. Production and Availability

The most common route is the cyclotron/accelerator-based production of ²¹¹At through alpha irradiation of ²⁰⁹Bi (natural Bi) via a ²⁰⁹Bi(α,2n)²¹¹At nuclear reaction [364,365]. However, only a limited number of cyclotrons with α-beam and with > 25 MeV energy are available in the field, limiting the overall ²¹¹At availability [364]. Other methods include the use of ²¹¹Rn/²¹¹At generators [366]. One of the potential advantages of using ²¹¹Rn/²¹¹At generators is the longer half-life of ²¹¹Rn (t_1/2 = 14.6 h) compared with ²¹¹At (t_1/2 = 7.2 h), facilitating wider distribution of ²¹¹At.

Table 32

Clinical applications of Astatine-211-labeled radiopharmaceuticals.

Radiopharmaceuticals	Targets	NCT Number ^	Disease
Sodium Astatide ([211At]NaAt)	-	NCT05275946 (Phase I; ongoing)	Thyroid cancer [367]
[211At]At- 81C6	Glial fibrillary acidic protein	NCT00003461 (Phase I/II; completed)	Metastatic cancer, brain and central nervous system tumors, neuroblastoma [368]
[211At]At- bc8-b10	CD45	NCT04083183 (Phase I/II; ongoing)NCT03670966 (Phase I/II; ongoing)	Non-malignant neoplasm [369]Acute lymphoblastic leukemia in remission [370]
[211At]At-OKT-B10	CD3	NCT04466475 (Phase I; ongoing)	Plasma cell myeloma [371]

NCT: National clinical trial, CD45: Cluster of differentiation, CD3: Cluster of differentiation 3, ^ clinicaltrials.gov and data accessed on 15 August 2023.

8. Conclusions

In summary, the development of radiometal-based radiopharmaceuticals, including their production, purification, bifunctional chelating agents, and biomarker discoveries, have significantly advanced the application of various radiometals in medicine in the last two decades. Both radiometal-based imaging and radionuclide therapy are changing the lives of patients on a daily basis due to the advancements made in the last 20 years. The field of α-emitting radiotherapy is emerging. Several clinical trials are currently under investigation. Further advances in the production and availability of these α-emitters along with the management of radioactive progeny should permit the cost-effective clinical adoption of TAT compared to traditional chemotherapeutics. Indeed, the future of the radiometal-based radiopharmaceutical industry appears to be very bright.

Author Contributions

S.S. and M.K.P. both contributed to manuscript writing, reviewing, and editing. All authors have read and agreed to the published version of the manuscript.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data are contained within the article.

Acknowledgments

The authors thank Desiree Lanzino for her assistance in editing the manuscript. The authors would like to thank the Division of Nuclear Medicine, Department of Radiology, Mayo Clinic, Rochester, MN, USA for financially supporting the writing of this manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

Abbreviations

Ab	=	Antibody
Ac	=	Actinium
AML	=	Acute myeloid leukemia
At	=	Astatine
Bi	=	Bismuth
CD8	=	Cluster of differentiation 8
CD38	=	Cluster of differentiation 38
CD20	=	Cluster of differentiation 20
CEA	=	Carcinoembryonic antigen
CERN	=	European Council for Nuclear Research
Cu	=	Copper
DaRT	=	Diffusing alpha-emitters radiation therapy
EC	=	Electron capture
EGFR	=	Epidermal growth factor receptor
FAPI	=	Fibroblast activation protein inhibitor
FDA	=	Food and Drug Administration
Ga	=	Gallium
GBM	=	Glioblastoma Multiforme
Ge	=	Germanium
GMP	=	Good manufacturing practice
HER2	=	Human epidermal growth factor 2
HOPO	=	2-hydroxypyridone-N-oxide
IART®	=	Intra-operative avidination for radionuclide therapy
ISOLDE	=	Isotope separation on-line
LET	=	Linear energy transfer
mAb	=	Monoclonal antibody
MAE	=	Meitner–Auger electrons
mCRPC	=	Metastatic castrate-resistant prostate cancer
MUC1	=	Mucin-1
NCT	=	National clinical trial
NET	=	Neuroendocrine tumor
Pb	=	Lead
PCa	=	Prostate cancer
PDL-1	=	Programmed cell death ligand-1
PET	=	Positron emission tomography
PRRT	=	Peptide receptor radionuclide therapy
PSA	=	Prostate-specific antigen
PSMA	=	Prostate-specific membrane antigen
Ra	=	Radium
Re	=	Rhenium
RIT	=	Radioimmunotherapy
Sc	=	Scandium
SPECT	=	Single photon emission computed tomography
SSTR2	=	Somatostatin-targeting receptor 2
SUV	=	Standardized uptake value
TAT	=	Targeted alpha therapy
Tb	=	Terbium
Tc	=	Technetium
Th	=	Thorium
Tl	=	Thallium
TRT	=	Targeted radionuclide therapy
TTC	=	Targeted thorium conjugates
U	=	Uranium
VEGF	=	Vascular endothelial growth factor
Y	=	Yttrium
Zn	=	Zinc
Zr	=	Zirconium

Footnotes

Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Figure, Schemes and Tables

Figure 1. Comparison of beta and alpha radionuclide therapies.

Scheme 1. Decay scheme for various radionuclides.

Scheme 2. Decay scheme for various α-emitting radionuclides.

Table 1

Decay characteristics of copper radioisotopes used in radiopharmaceuticals ^#.

Isotope	Half-Life (t1/2)	Decay Characteristics	Energies		Eγ; keV(Intensity %)
			Eβ+avg(keV)	Eβ−avg(keV)
60Cu	23.7 min	β+ = 93%EC = 7%	970	-	1332.5 (88)1791.6 (45.4)826.4 (21.7)
61Cu	3.33 h	β+ = 61%EC = 39%	500	-	282.95 (12.7)656 (10.4)
62Cu	9.76 min	β+ = 97%EC = 2%	2910	-	511 (194)
64Cu	12.70 h	β− = 38.5%β+ = 17.6%EC = 43.9%	278	191	1345.77 (0.475)
67Cu	61.83 h	β− = 100%	-	141	184.57 (48.7)

^# Data on ^60/61/64/67Cu are from [24] and data on ⁶²Cu are from [25]. Please refer to Scheme 1A.

Table 2

Clinical applications of various ^64/67Cu-labeled radiopharmaceuticals.

Radiopharmaceuticals	Targets	NCT Number ^	Disease [Ref.]
[64Cu]Cu-ATSM	Hypoxia-targeted	NCT03951337 (Phase II; ongoing)	Rectum cancer [32]
[64Cu]Cu-DOTA-trastuzumab	HER2+	NCT02827877 (Phase II; ongoing)	Breast cancer [56]
[64Cu]Cu-DOTA-M5A	CEA	NCT05245786 (Early phase I; ongoing)	Rectal cancer [57]
[64Cu]Cu- SarTATE	SSTR	NCT04438304 (Phase II; ongoing)	Neuroendocrine tumors [58]
[64Cu]Cu-TP3805	VPAC1	NCT02603965; (Phase I; completed)	Prostate cancer [59]
[64Cu]Cu-DOTA-AE105	uPAR	NCT02139371 (Early phase I; completed)	Breast, prostate, and bladder cancer [60]
[64Cu]Cu-SAR-bisPSMA	PSMA	NCT04839367 (Phase I; completed)NCT05249127 (Phase I/II; ongoing)	Prostate neoplasms [61]Recurrent prostate neoplasm [62]
[67Cu]Cu- SarTATE	SSTR	NCT03936426 (Phase I/IIa; completed),NCT04023331 (Phase I/IIa; ongoing)	Meningioma [63]Neuroblastoma [64]
[64/67Cu]Cu-SAR-bisPSMA	PSMA	NCT04868604 (Phase I/IIa; ongoing)	Castration-resistant prostate cancer [65]

NCT: National clinical trial, uPAR: Urokinase plasminogen activator receptor, CEA: Carcinoembryonic antigen, VPAC1: Vasoactive intestinal polypeptide, SSTR: Somatostatin receptors, PSMA: Prostate-specific membrane antigen, ^ clinicaltrials.gov and data accessed on 15 August 2023.

Table 3

Decay characteristics of the three main radioisotopes of gallium ^#.

Isotope	Half-Life (t1/2)	Decay Characteristics	Energy	Eγ; keV (Intensity %)
			Eβ+avg (keV)
66Ga	9.49 h	β+ = 57%	1750	1039.22 (37)
		EC = 43%
67Ga	3.26 d	EC = 100%	-	93.31 (38.81)
68Ga	67.71 min	β+ = 88.91%	829.5	1077.34 (3.22)
		EC = 11.09%

^# Data on ^66/67/68Ga are from [78]. Please refer to Scheme 1A.

Table 7

Decay characteristics of commonly used radioisotopes of scandium ^#.

Isotope	Half-Life (t1/2)	Decay Characteristics	Energies		Eγ; keV (Intensity%)
			Eβ+avg (keV)	Eβ−avg (keV)
43Sc	3.9 h	β+ = 88%	476	-	372(23)
		EC = 12%
44Sc	4.0 h	β+ = 94%	632	-	1157(100)
		EC = 6%
47Sc	3.35 d	β− = 100%	-	162	159(68)

^#Data on ^43/44/47Sc are from [150]. Please refer to decay Scheme 1A.

Table 8

Decay characteristics of leading radioisotopes of terbium ^#.

Isotope	Half-Life (t1/2)	Decay Characteristics	Energy			Eγ; keV (Intensity %)
			Eβ+avg (keV)	Eα avg(keV)	Eβ-avg(keV)
149Tb(α-therapy)	4.12 h	α = 16.7%	730	3967	-	165 (26), 352 (29)
		β+ = 7.1%				388.6 (18)
		EC = 76.2%				652.1 (16)
152Tb(PET)	17.5 h	β+ = 17%	1080	-	-	344.3 (65)
		EC = 83%				586.3(9.4)
155Tb(SPECT)	5.32 d	EC = 100%	-	-	-	86.55 (32)
						105.3 (25)
161Tb(β−/MAE therapy)	6.89 d	β− = 100%	-	-	154	25.65 (23)
						48.92 (17)
						74.57 (10)

^# Data on ^{149/152/155/161}Tb are from [167]. Please refer to decay Scheme 1A.

Table 9

Clinical applications of terbium-labeled radiopharmaceuticals under investigation.

Radiopharmaceuticals	Targets	NCT Number ^	Disease
[161Tb]Tb-DOTA-LM3	SSTR2	NCT05359146 (Early phase 1; recruiting)	Neuroendocrine neoplasia or gastroenteropancreatic neuroendocrine tumor [179]
[161Tb]Tb-PSMA-I&T	PSMA	NCT05521412 (Phase I/II; recruiting)	Prostate cancer or metastatic castration-resistant prostate cancer [180]

NCT: National clinical trial, SSTR2: Somatostatin-targeting receptor 2, PSMA: Prostate-specific membrane antigen, ^ clinicaltrials.gov and data accessed on 15 August 2023.

Table 10

Decay characteristics of PET isotopes of zinc ^#.

Isotope	Half-Life (t1/2)	Decay Characteristics	Eβ+avg (keV)	Eγ; keV (Intensity%)
62Zn	9.26 h	β+ = 8.2%	259	508 (15), 550 (15)
				600 (26)
63Zn	38.47 min	β+ = 93%	992	670 (8)
				960 (7)
65Zn	243.9 d	β+ = 98%	142.5	1110 (50.6)

^# Data on ^62/63/65Zn are from [184]. Please refer to Scheme 1A.

Table 11

Decay characteristics of Technetium-99 m ^#.

Isotope	Half-Life (t1/2)	Decay Characteristics	Eγ; keV (Intensity %)
99mTc	6.0 h	IT = 100%	140.51 (98.6)

^# Data on ^99mTc are from [188]. Please refer to Scheme 1B.

Table 12

Clinical applications of ^99mTc-labeled radiopharmaceuticals.

Radiopharmaceuticals	Targets	NCT Number ^	Disease
[99mTc]Tc-tilmanocept	Lymph node	NCT02201420 (Phase II; completed)	Kaposi’s sarcoma [196]
[99m Tc]Tc-EC20	Folate	NCT01689714 (Phase II; completed)	Ovarian or recurrent endometrial carcinoma [197]
[99m Tc]Tc-Tetrofosmin	-	NCT02971319 (Phase II; completed)	Glioma [198]
[99m Tc]Tc-Sestamibi	-	NCT05042687 (Phase not applicable)	Breast cancer [199]
[99m Tc]Tc-HYNIC TOC EDAA	SSTR	NCT02691078 (Phase II completed)	Neuroendocrine tumors [200]
[99m Tc]Tc-MP-1404	PSMA	NCT02615067 (Phase III completed)	Prostate cancer [201]
[99m Tc]Tc-MP-1404[99m Tc]Tc-MP-1405	PSMA	NCT01261754 (Phase I; completed)	Prostate cancer [202]
[99m Tc]Tc-PSMA I&S	PSMA	NCT04832958 (Phase II; ongoing)	Prostate cancer [203]
[99m Tc]Tc-labeled albumin in macroaggregates (MAA) and in microspheres (B20)	-	NCT01186263(Phase II; completed)	Colorectal cancer, liver metastasis [204]

NCT: National clinical trial, PSMA: Prostate-specific molecular antigen, SSTR: Somatostatin-targeting receptor, ^ clinicaltrials.gov and data accessed on 15 August 2023.

Table 13

Decay characteristics of radioisotopes of indium ^#.

Isotope	Half-Life (t1/2)	Decay Characteristics	Eβ+avg (keV)	Eγ; keV (Intensity %)
110mIn	69.1min	β+ = 61.3%	1011	657.75(97.74)
		EC = 38%
111In	2.8 d	EC = 100%	-	245.35(94.1)

^# Data on ^110m/111In are from [78]. Please refer to Scheme 1B.

Table 15

Decay characteristics of Yttrium-90 ^#.

Isotope	Half-Life (t1/2)	Decay Characteristics	Energy
			Eβ−max (MeV)	Eβ−avg (MeV)
90Y	64.0 h	β− = 100%	2.284	0.933

^# Data on ⁹⁰Y are from [223]. Please refer to Scheme 1C.

Table 17

Decay characteristics of ^186/188Re ^#.

Isotope	Half-Life (t1/2)	Decay Characteristics	Eβ−avgKeV	Eγ; keV (Intensity%)
186Re	90 h	β− = 92.59%	346.7	137.15 (9.47)
		EC = 7.41%		106 (12.1)
188Re	17.0 h	β− = 100%	763	155.04 (15.49)
				478 (1.076%)

^# Data on ^186/188Re are from [251]. Please refer to Scheme 1C.

Table 19

Decay characteristics of Lutetium-177 ^#.

Isotope	Half-Life (t1/2)	Eβ-max (keV)	Eϒ; keV (Intensity%)
177Lu	6.647 d	497 (78.6%)384 (9.1%)176 (12.2%)	208 (11%)113 (6.6%)

^# Data on ¹⁷⁷Lu are from [271]. Please refer to decay Scheme 1C.

Table 20

Clinical applications of ¹⁷⁷Lu-labeled radiopharmaceuticals.

Radiopharmaceuticals	Targets	NCT Number ^	Disease [Ref.]
[177Lu]Lu-PSMA-617 (PLUVICTO ®)	PSMA	NCT03511664 (Phase III; ongoing)	Metastatic prostate cancer [279]
223Ra + [177Lu]Lu-PSMA- I & T	PSMA	NCT05383079 (phase II; recruiting)	Metastatic castration-resistant prostate cancer [280]
Cabozantinib in Combination With [177Lu]Lu- DOTATATE (LuTATE)	SSTR2	NCT05249114 (Phase Ib; ongoing)	Neuroendocrine tumors [281]
capecitabine (CAP)/temozolomide (TEM) + [177Lu]Lu- DOTATATE (LuTATE)	SSTR	NCT02358356 (phase II; completed)	Mid gut or pancreatic neuroendocrine tumors [282]
[177Lu]Lu- DOTATATE (Lutathera)	SSTR2	NCT03206060 (phase II; ongoing)	Pheochromocytoma/Paraganglioma, neuroendocrine tumor [283]
[177Lu]Lu-Edotreotide	SSTR	NCT04919226 (phase III; ongoing)	Gastroenteropancreatic neuroendocrine tumors [284]
[177Lu]Lu- catalase	-	NCT05985278 (Early phase 1; ongoing)	Advanced malignant neoplasm [285]
[177Lu]Lu-EB-FAPI	FAP	NCT05400967 (Early phase 1; ongoing)	Metastatic tumors [286]
[177Lu]Lu-DOTA-girentuximab	Carbonic anhydrase IX	NCT02002312 (phase II; completed)	Metastatic clear cell renal cancer [287]

NCT: National clinical trial, SSTR: Somatostatin receptors, FAP: Fibroblast activation protein, ^ clinicaltrials.gov and data accessed on 15 August 2023.

Table 23

Decay characteristics of Actinimum-225 ^#.

Isotope	Half-Life (t1/2)	Decay Characteristics	225Ac and Daughter Nuclides	Energies (MeV)		Eγ; keV (Intensity%)
				Eαmax	Eβ− max
225Ac	9.9 d	α = 100%	225Ac221Fr217At213Bi213Po209Tl209Pb209Bi (stable)	225Ac-5.8221Fr-6.3217At-7.1213Bi-5.9213Po-8.4	213Bi-0.492209Tl-0.178209Pb-0.198	213Bi-100 (1)221Fr-218 (11.4)213Bi-440 (26)209Tl-1567 (99.7)

^# Data on ²²⁵Ac are from [300]. Please refer to Scheme 2.

Table 24

Clinical application of Actinium-225-labeled radiopharmaceuticals.

Radiopharmaceuticals	Targets	NCT Number ^	Disease
[225Ac]Ac-lintuzumab with Venetoclax	BCL-2	NCT03867682 (Phase I/II; ongoing)	Acute and relapsed myeloid leukemia [304]
[225Ac]Ac-DOTA-Daratumumab	CD38	NCT05363111 (Phase I; ongoing)	Recurrent plasma cell myeloma [305]
[225Ac]Ac-FPI-1434	IGF-1R	NCT03746431 (Phase I/II; ongoing)	Advanced solid tumor, endometrial cancer, ovarian, cervical cancer [306]
[225Ac]Ac-DOTA-M5A	CEA	NCT05204147 (Phase I; ongoing)	Advanced and metastatic cancer [307]
[225Ac]Ac-PSMA-617	PSMA	NCT04597411(Phase I; ongoing)	Castration-resistant prostate cancer [308]
[225Ac]Ac-J591	PSMA	NCT03276572 (Phase I; ongoing)	Prostate cancer [309]

NCT: National clinical trial, BCL-2: B-cell lymphoma 2, CD38: Cluster of differentiation 38, IGF-1R: Type 1 insulin-like growth factor receptor, CEA: Carcinoembryonic antigen, PSMA: Prostate-specific membrane antigen, ^ clinicaltrials.gov and data accessed on 15 August 2023.

Table 25

Decay characteristics of lead ^#.

Isotope	Half-Life (t1/2)	Decay Characteristics	Parent and Daughter Nuclides	Energies		Eγ; keV; (Intensity%)
				Eαmax(MeV)	Eβ- max(MeV)
203Pb	51.9h	EC = 100%	203Tl (stable)	-	-	279 (81)
212Pb	10.6h	β− = 100%	212Pb212Bi212Po208Tl208Pb (stable)	212Bi-6.1212Po-8.8	212Pb-0.102212Bi-0.769208Tl-0.557	212Pb-238.6 (43.6)212Bi-727.3 (6.6)208Tl-277.4 (6.3), 510.8 (22.6), 583.2 (84.5), 763 (1.8), 860.6 (12.4), 2614.5 (99.2)

^# Data on ²⁰³Pb are from [312] and data on ²¹²Pb are from [296]. Please refer to Scheme 2.

Table 26

Clinical applications of ²¹²lead-212-labeled radiopharmaceuticals.

Radiopharmaceuticals	Targets	NCT Number ^	Disease
[212Pb]Pb-DOTAMTATE (Alpha MedixTM)	SSTR	NCT05153772(Phase II; ongoing)	Neuroendocrine tumors [317]
[212Pb]Pb-TCMC-Trastuzumab	HER2+	NCT01384253(Phase I; completed)	Breast, ovarian, peritoneal, pancreatic, and stomach neoplasm [318]

NCT: National clinical trial, SSTR: Somatostatin receptors, HER2+: Human epidermal growth factor 2, ^ clinicaltrials.gov and data accessed on 15 August 2023.

Table 28

Clinical applications of ²²³Ra in combination with other therapies.

Radiopharmaceuticals	Targets	NCT Number ^	Disease
[223Ra]Ra-dichloride	Skeletal metastasis	NCT01833520 (Phase II; completed)	Sarcoma [337]
[223Ra]Ra-dichloride + Niraparib	PARP inhibitor	NCT03076203 (Phase I; completed)	Prostate cancer metastatic to bone, stage IV prostate cancer, hormone refractory prostate cancer [338]
[223Ra]Ra-dichloride + Abiraterone, Prednisone/Prednisolone	CYP17 inhibitor	NCT02043678 (Phase III; active)	Prostate cancer [339]
[223Ra]Ra-dichloride + Enzalutamide	AR inhibitor	NCT02199197 (Phase II; completed) NCT03305224 (Phase II; ongoing)	Prostate cancer, [340], bone metastatic prostate cancer [341]
[223Ra]Ra-dichloride + Denosumab	Cytokine RANKL	NCT02366130 (Phase II; completed)	Breast carcinoma [342]
[223Ra]Ra-dichloride + Paclitaxel	Tubulin	NCT02442063 (Phase I; completed)	Neoplasm, bone disease [343]
[223Ra]Ra-dichloride + Docetaxel	P300	NCT03574571 (Phase III; ongoing)	Prostate cancer [344]
[223Ra]Ra-dichloride + Leuprolide acetate,	GnRH-receptor agonist	NCT03361735 (Phase II; ongoing)	Prostate cancer [345]
[223Ra]Ra-dichloride + Pembrolizumab	PDL-1	NCT03093428 (Phase II; ongoing)	Prostate cancer [346]
[223Ra]Ra-dichloride + Atezolizumab	PDL-1	NCT02814669 (Phase I; completed)	Castration-resistant prostate cancer [347]
Alpha-DaRT seeds (224Ra containing 316LVM tubes)	Implantation sites	NCT04002479 (Phase not applicable) NCT03970967 (Phase not applicable)	Metastatic pancreatic cancer [348]Metastatic breast cancer [349]

NCT: National clinical trial, PARP: Polyadenosine diphosphate-ribose polymerase, CYP17: Cytochrome P450 17 α-hydroxylase/17,20-lyase, AR inhibitor: Androgen receptors, RANKL: Receptor activator of nuclear factor-kB ligand, GnRH-receptor agonist: Gonadotropin-releasing hormone, PDL-1: Programmed cell death ligand-1, ^ clinicaltrials.gov and data accessed on 15 August 2023.

Table 29

Decay characteristics of thorium-227, which follow a decay chain of radium-223 ^#.

Isotope	Half-Life (t1/2)	227Th and Daughter Nuclides		Eαmax (MeV)
				Eαmax	Eβ−max
227Th	18.7 d	α = 100%	227Th223Ra219Rn215Po211Pb211Bi207Tl211Po207Pb (stable)	227Th-5.9223Ra-5.7219Rn-6.8215Po-7.4211Bi-6.6211Po-7.6	211Pb-0.4211Bi-0.6207Tl-0.5

^# Data on ²²⁷Th are from [354]. Please refer to Scheme 2.

Table 31

Decay characteristics of Astatine-221 ^#.

Isotope	Half-Life (t1/2)	Decay Characteristics	211At and Daughter Nuclides	Eαmax(MeV)	Eγ; keV
211At	7.2 h	α = 42%EC = 58%	207Bi211Po207Pb (stable)	5.877.45	211At-687211Po-569.7, 897.8

^# Data on ²¹¹At are from [361]. Please refer to Scheme 2.

References

1. Gianfaldoni, S.; Gianfaldoni, R.; Wollina, U.; Lotti, J.; Tchernev, G.; Lotti, T. An Overview on Radiotherapy: From Its History to Its Current Applications in Dermatology. Open Access Maced. J. Med. Sci.; 2017; 5, pp. 521-525. [DOI: https://dx.doi.org/10.3889/oamjms.2017.122]

2. Lederman, M. The early history of radiotherapy: 1895–1939. Int. J. Radiat. Oncol. Biol. Phys.; 1981; 7, pp. 639-648. [DOI: https://dx.doi.org/10.1016/0360-3016(81)90379-5]

3. Pusey, W.A. Roentgen rays in the treatment of skin diseases and for the removal of hair. J. Cutan. Dis. Incl. Syph.; 1900; 18, pp. 302-318. [DOI: https://dx.doi.org/10.1001/archderm.1983.01650260070022]

4. Chen, H.H.W.; Kuo, M.T. Improving radiotherapy in cancer treatment: Promises and challenges. Oncotarget; 2017; 8, pp. 62742-62758. [DOI: https://dx.doi.org/10.18632/oncotarget.18409]

5. Jones, P.M. Treatment of lupus by x-rays. Phila. Med. J.; 1900; 5, pp. 63-64.

6. Majeed, H.; Gupta, V. Adverse Effects of Radiation Therapy; StatPearls Publishing LLC.: Treasure Island, FL, USA, 2023.

7. Kerr, C.P.; Grudzinski, J.J.; Nguyen, T.P.; Hernandez, R.; Weichert, J.P.; Morris, Z.S. Developments in Combining Targeted Radionuclide Therapies and Immunotherapies for Cancer Treatment. Pharmaceutics; 2023; 15, 128. [DOI: https://dx.doi.org/10.3390/pharmaceutics15010128]

8. Rafael, M.S.; Cohen-Gogo, S.; Irwin, M.S.; Vali, R.; Shammas, A.; Morgenstern, D.A. Theranostics in Neuroblastoma. PET Clin.; 2021; 16, pp. 419-427. [DOI: https://dx.doi.org/10.1016/j.cpet.2021.03.006]

9. Cimini, A.; Ricci, M.; Chiaravalloti, A.; Filippi, L.; Schillaci, O. Theragnostic Aspects and Radioimmunotherapy in Pediatric Tumors. Int. J. Mol. Sci.; 2020; 21, 3849. [DOI: https://dx.doi.org/10.3390/ijms21113849]

10. Elisa Crestoni, M. Radiopharmaceuticals for diagnosis and therapy. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering; Elsevier: Amsterdam, The Netherlands, 2018; [DOI: https://dx.doi.org/10.1016/B978-0-12-409547-2.14205-2]

11. Schmitz, R.E.; Alessio, A.M.; Kinahan, P. The Physics of PET/CT Scanners; University of Washington: Washington, DC, USA, 2013.

12. Chen, I.Y.; Wu, J.C. Cardiovascular molecular imaging: Focus on clinical translation. Circulation; 2011; 123, pp. 425-443. [DOI: https://dx.doi.org/10.1161/CIRCULATIONAHA.109.916338]

13. Van Audenhaege, K.; Van Holen, R.; Vandenberghe, S.; Vanhove, C.; Metzler, S.D.; Moore, S.C. Review of SPECT collimator selection, optimization, and fabrication for clinical and preclinical imaging. Med. Phys.; 2015; 42, pp. 4796-4813. [DOI: https://dx.doi.org/10.1118/1.4927061]

14. Rahmim, A.; Zaidi, H. PET versus SPECT: Strengths, limitations, and challenges. Nucl. Med. Commun.; 2008; 29, pp. 193-207. [DOI: https://dx.doi.org/10.1097/MNM.0b013e3282f3a515] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/18349789]

15. Ljungberg, M.; Pretorius, P.H. SPECT/CT: An update on technological developments and clinical applications. Br. J. Radiol.; 2018; 91, 20160402. [DOI: https://dx.doi.org/10.1259/bjr.20160402]

16. Single Photon Emission Computed Tomography (SPECT). Available online: https://cmgi.ucdavis.edu/services/single-photon-emission-computed-tomography-spect/ (accessed on 15 August 2023).

17. Lu, F.M.; Yuan, Z. PET/SPECT molecular imaging in clinical neuroscience: Recent advances in the investigation of CNS diseases. Quant. Imaging Med. Surg.; 2015; 5, pp. 433-447. [DOI: https://dx.doi.org/10.3978/j.issn.2223-4292.2015.03.16] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/26029646]

18. Bast, R.C., Jr.; Kousparou, C.A.; Epenetos, A.A.; Zalutsky, M.R.; Kreitman, R.J.; Sausville, E.A.; Frankel, A.E. Radioimmunotherapy of Cancer. Holland-Frei Cancer Medicine; 6th ed. Decker: Hamilton, ON, Canada, 2003; Available online: https://www.ncbi.nlm.nih.gov/books/NBK13926/ (accessed on 15 August 2023).

19. Sofou, S. Radionuclide carriers for targeting of cancer. Int. J. Nanomed.; 2008; 3, pp. 181-199. [DOI: https://dx.doi.org/10.2147/IJN.S2736] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/18686778]

20. Álvarez N., H.; Hernández-Gil, J.; Lewis, J.S. Recent Advances in Radiometals for Combined Imaging and Therapy in Cancer. ChemMedChem; 2021; 16, 2909. [DOI: https://dx.doi.org/10.1002/cmdc.202100135]

21. De Kruijff, R.M.; Wolterbeek, H.T.; Denkova, A.G. A Critical Review of Alpha Radionuclide Therapy-How to Deal with Recoiling Daughters?. Pharmaceuticals; 2015; 8, pp. 321-336. [DOI: https://dx.doi.org/10.3390/ph8020321]

22. Aghevlian, S.; Boyle, A.J.; Reilly, R.M. Radioimmunotherapy of cancer with high linear energy transfer (LET) radiation delivered by radionuclides emitting α-particles or Auger electrons. Adv. Drug Deliv. Rev.; 2017; 109, pp. 102-118. [DOI: https://dx.doi.org/10.1016/j.addr.2015.12.003] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/26705852]

23. De Nardo, L.; Pupillo, G.; Mou, L.; Esposito, J.; Rosato, A.; Meléndez-Alafort, L. A feasibility study of the therapeutic application of a mixture of 67/64Cu radioisotopes produced by cyclotrons with proton irradiation. Med. Phys.; 2022; 49, pp. 2709-2724. [DOI: https://dx.doi.org/10.1002/mp.15524] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/35134261]

24. Mou, L.; Martini, P.; Pupillo, G.; Cieszykowska, I.; Cutler, C.S.; Mikołajczak, R. ⁶⁷Cu Production Capabilities: A Mini Review. Molecules; 2022; 27, 1501. [DOI: https://dx.doi.org/10.3390/molecules27051501]

25. Ling, X.; Cutler, C.S.; Anderson, C.J. The radiopharmaceutical chemistry of the radioisotopes of copper. Radiopharmaceutical Chemistry; Lewis, J.S.; Windhorst, A.D.; Zeglis, B.M. Springer International Publishing: Cham, Switzerland, 2019; pp. 335-358. [DOI: https://dx.doi.org/10.1007/978-3-319-98947-1_19]

26. Fujibayashi, Y.; Taniuchi, H.; Yonekura, Y.; Ohtani, H. Copper-62-ATSM: A new hypoxia imaging agent with high membrane permeability and low redox potential. J. Nucl. Med.; 1997; 38, 1155.

27. Lewis, J.S.; Laforest, R.; Dehdashti, F.; Grigsby, P.W.; Welch, M.J.; Siegel, B.A. An imaging comparison of 64Cu-ATSM and 60Cu-ATSM in cancer of the uterine cervix. J. Nucl. Med.; 2008; 49, pp. 1177-1182. [DOI: https://dx.doi.org/10.2967/jnumed.108.051326] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/18552145]

28. Colombié, M.; Gouard, S.; Frindel, M.; Vidal, A.; Chérel, M.; Kraeber-Bodéré, F.; Rousseau, C.; Bourgeois, M. Focus on the Controversial Aspects of (64)Cu-ATSM in Tumoral Hypoxia Mapping by PET Imaging. Front. Med.; 2015; 2, 58. [DOI: https://dx.doi.org/10.3389/fmed.2015.00058]

29. Dehdashti, F.; Grigsby, P.W.; Lewis, J.S.; Laforest, R.; Siegel, B.A.; Welch, M.J. Assessing tumor hypoxia in cervical cancer by PET with 60Cu-labeled diacetyl-bis (N4-methylthiosemicarbazone). J. Nucl. Med.; 2008; 49, pp. 201-205. [DOI: https://dx.doi.org/10.2967/jnumed.107.048520]

30. Dietz, D.W.; Dehdashti, F.; Grigsby, P.W.; Malyapa, R.S.; Myerson, R.J.; Picus, J.; Ritter, J.; Lewis, J.S.; Welch, M.J.; Siegel, B.A. Tumor hypoxia detected by positron emission tomography with 60Cu-ATSM as a predictor of response and survival in patients undergoing neoadjuvant chemoradiotherapy for rectal carcinoma: A pilot study. Dis. Colon Rectum; 2008; 51, pp. 1641-1648. [DOI: https://dx.doi.org/10.1007/s10350-008-9420-3]

31. Tateishi, K.; Tateishi, U.; Sato, M.; Yamanaka, S.; Kanno, H.; Murata, H.; Inoue, T.; Kawahara, N. Application of 62Cu-diacetyl-bis (N4-methylthiosemicarbazone) PET imaging to predict highly malignant tumor grades and hypoxia-inducible factor-1α expression in patients with glioma. Am. J. Neuroradiol.; 2013; 34, pp. 92-99. [DOI: https://dx.doi.org/10.3174/ajnr.A3159]

32. 64Cu-ATSM PET/CT in Rectum Cancer (TEP 64Cu-ATSM-Rectum). Available online: https://clinicaltrials.gov/search?term=NCT03951337 (accessed on 20 August 2023).

33. Lewis, J.S.; Laforest, R.; Buettner, T.L.; Song, S.-K.; Fujibayashi, Y.; Connett, J.M.; Welch, M.J. Copper-64-diacetyl-bis(N⁴-methylthiosemicarbazone): An agent for radiotherapy. Proc. Natl. Acad. Sci. USA; 2001; 98, pp. 1206-1211. [DOI: https://dx.doi.org/10.1073/pnas.98.3.1206]

34. Yoshii, Y.; Furukawa, T.; Yoshimoto, M.; Matsumoto, H.; Zhang, M.-R.; Inubushi, M.; Fujibayashi, Y.; Saga, T. ⁶⁴Cu-ATSM internal radiotherapy to treat colon cancer acquiring resistance to antiangiogenic therapy with bevacizumab. J. Nucl. Med.; 2016; 57, 471.

35. Yoshii, Y.; Furukawa, T.; Kiyono, Y.; Watanabe, R.; Mori, T.; Yoshii, H.; Asai, T.; Okazawa, H.; Welch, M.J.; Fujibayashi, Y. Internal radiotherapy with copper-64-diacetyl-bis (N4-methylthiosemicarbazone) reduces CD133+ highly tumorigenic cells and metastatic ability of mouse colon carcinoma. Nucl. Med. Biol.; 2011; 38, pp. 151-157. [DOI: https://dx.doi.org/10.1016/j.nucmedbio.2010.08.009] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/21315269]

36. Fujibayashi, Y.; Yoshii, Y.; Furukawa, T.; Yoshimoto, M.; Matsumoto, H.; Saga, T. Imaging and therapy against hypoxic tumors with 64Cu-ATSM. Make Life Visible; Springer: Singapore, 2020; pp. 285-292.

37. Anderson, C.J.; Connett, J.M.; Schwarz, S.W.; Rocque, P.A.; Guo, L.W.; Philpott, G.W.; Zinn, K.R.; Meares, C.F.; Welch, M.J. Copper-64-labeled antibodies for PET imaging. J. Nucl. Med.; 1992; 33, pp. 1685-1691.

38. Suzuki, H.; Kise, S.; Kaizuka, Y.; Watanabe, R.; Sugawa, T.; Furukawa, T.; Fujii, H.; Uehara, T. Copper-64-Labeled Antibody Fragments for Immuno-PET/Radioimmunotherapy with Low Renal Radioactivity Levels and Amplified Tumor-Kidney Ratios. ACS Omega; 2021; 6, pp. 21556-21562. [DOI: https://dx.doi.org/10.1021/acsomega.1c02516] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/34471758]

39. Bryan, J.N.; Lewis, M.R.; Henry, C.J.; Owen, N.K.; Zhang, J.; Mohsin, H.; Jia, F.; Sivaguru, G.; Anderson, C.J. Development of a two-antibody model for the evaluation of copper-64 radioimmunotherapy. Vet. Comp. Oncol.; 2004; 2, pp. 82-90. [DOI: https://dx.doi.org/10.1111/j.1476-5810.2004.00041.x] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/19379188]

40. Mortimer, J.E.; Bading, J.R.; Colcher, D.M.; Conti, P.S.; Frankel, P.H.; Carroll, M.I.; Tong, S.; Poku, E.; Miles, J.K.; Shively, J.E. et al. Functional imaging of human epidermal growth factor receptor 2-positive metastatic breast cancer using (64)Cu-DOTA-trastuzumab PET. J. Nucl. Med.; 2014; 55, pp. 23-29. [DOI: https://dx.doi.org/10.2967/jnumed.113.122630]

41. Mortimer, J.E.; Bading, J.R.; Park, J.M.; Frankel, P.H.; Carroll, M.I.; Tran, T.T.; Poku, E.K.; Rockne, R.C.; Raubitschek, A.A.; Shively, J.E. et al. Tumor Uptake of (64)Cu-DOTA-Trastuzumab in Patients with Metastatic Breast Cancer. J. Nucl. Med.; 2018; 59, pp. 38-43. [DOI: https://dx.doi.org/10.2967/jnumed.117.193888] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/28637802]

42. Krenning, E.P.; Kwekkeboom, D.J.; Bakker, W.H.; Breeman, W.A.P.; Kooij, P.P.M.; Oei, H.Y.; van Hagen, M.; Postema, P.T.E.; de Jong, M.; Reubi, J.C. et al. Somatostatin receptor scintigraphy with [111In-DTPA-d-Phe1]- and [123I-Tyr3]-octreotide: The Rotterdam experience with more than 1000 patients. Eur. J. Nucl. Med.; 1993; 20, pp. 716-731. [DOI: https://dx.doi.org/10.1007/BF00181765] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/8404961]

43. Gabriel, M.; Muehllechner, P.; Decristoforo, C.; Guggenberg, E.v. 99-mTc-EDDA/HYNIC-Tyr (3)-octreotide for staging and follow-up of patients with neuroendocrine gastro-entero-pancreatic tumors. Q. J. Nucl. Med. Mol. Imaging; 2005; 49, pp. 237-244. [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/16172569]

44. Hofmann, M.; Maecke, H.; Börner, A.; Weckesser, E.; Schöffski, P.; Oei, M.; Schumacher, J.; Henze, M.; Heppeler, A.; Meyer, G. Biokinetics and imaging with the somatostatin receptor PET radioligand 68Ga-DOTATOC: Preliminary data. Eur. J. Nucl. Med.; 2001; 28, pp. 1751-1757. [DOI: https://dx.doi.org/10.1007/s002590100639]

45. Pfeifer, A.; Knigge, U.; Mortensen, J.; Oturai, P.; Berthelsen, A.K.; Loft, A.; Binderup, T.; Rasmussen, P.; Elema, D.; Klausen, T.L. et al. Clinical PET of Neuroendocrine Tumors Using ⁶⁴Cu-DOTATATE: First-in-Humans Study. J. Nucl. Med.; 2012; 53, pp. 1207-1215. [DOI: https://dx.doi.org/10.2967/jnumed.111.101469]

46. Johnbeck, C.B.; Knigge, U.; Loft, A.; Berthelsen, A.K.; Mortensen, J.; Oturai, P.; Langer, S.W.; Elema, D.R.; Kjaer, A. Head-to-Head Comparison of (64)Cu-DOTATATE and ⁶⁸Ga-DOTATOC PET/CT: A Prospective Study of 59 Patients with Neuroendocrine Tumors. J. Nucl. Med.; 2017; 58, pp. 451-457. [DOI: https://dx.doi.org/10.2967/jnumed.116.180430]

47. Loft, M.; Carlsen, E.A.; Johnbeck, C.B.; Johannesen, H.H.; Binderup, T.; Pfeifer, A.; Mortensen, J.; Oturai, P.; Loft, A.; Berthelsen, A.K. et al. ⁶⁴Cu-DOTATATE PET in Patients with Neuroendocrine Neoplasms: Prospective, Head-to-Head Comparison of Imaging at 1 Hour and 3 Hours After Injection. J. Nucl. Med.; 2021; 62, pp. 73-80. [DOI: https://dx.doi.org/10.2967/jnumed.120.244509]

48. FDA Approves Radioactive Diagnostic Agent Detectnet. Available online: https://www.empr.com/home/news/detectnet-approved-positron-emission-tomography-pet-neuroendocrine-tumors/#:~:text=The%20Food%20and%20Drug%20Administration (accessed on 15 August 2023).

49. SARTATE Clinical Development. Available online: https://www.claritypharmaceuticals.com/pipeline/sartate/ (accessed on 15 August 2023).

50. Horoszewicz, J.S.; Kawinski, E.; Murphy, G.P. Monoclonal antibodies to a new antigenic marker in epithelial prostatic cells and serum of prostatic cancer patients. Anticancer Res.; 1987; 7, pp. 927-935.

51. Hosseindokht, M.; Bakherad, H.; Zare, H. Nanobodies: A tool to open new horizons in diagnosis and treatment of prostate cancer. Cancer Cell Int.; 2021; 21, 580. [DOI: https://dx.doi.org/10.1186/s12935-021-02285-0] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/34717636]

52. Novakova, Z.; Belousova, N.; Foss, C.A.; Havlinova, B.; Gresova, M.; Das, G.; Lisok, A.; Prada, A.; Barinkova, M.; Hubalek, M. et al. Engineered Fragments of the PSMA-Specific 5D3 Antibody and Their Functional Characterization. Int. J. Mol. Sci.; 2020; 21, 6672. [DOI: https://dx.doi.org/10.3390/ijms21186672] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/32932591]

53. Grubmüller, B.; Baum, R.P.; Capasso, E.; Singh, A.; Ahmadi, Y.; Knoll, P.; Floth, A.; Righi, S.; Zandieh, S.; Meleddu, C. et al. (64)Cu-PSMA-617 PET/CT Imaging of Prostate Adenocarcinoma: First In-Human Studies. Cancer Biother. Radiopharm.; 2016; 31, pp. 277-286. [DOI: https://dx.doi.org/10.1089/cbr.2015.1964]

54. Afshar-Oromieh, A.; Zechmann, C.M.; Malcher, A.; Eder, M.; Eisenhut, M.; Linhart, H.G.; Holland-Letz, T.; Hadaschik, B.A.; Giesel, F.L.; Debus, J. et al. Comparison of PET imaging with a ⁶⁸Ga-labelled PSMA ligand and (18)F-choline-based PET/CT for the diagnosis of recurrent prostate cancer. Eur. J. Nucl. Med. Mol. Imaging; 2014; 41, pp. 11-20. [DOI: https://dx.doi.org/10.1007/s00259-013-2525-5]

55. Denardo, G.L.; Denardo, S.J.; Kukis, D.L.; O’Donnell, R.T.; Shen, S.; Goldstein, D.S.; Kroger, L.A.; Salako, Q.; Denardo, D.A.; Mirick, G.R. et al. Maximum tolerated dose of 67Cu-2IT-BAT-LYM-1 for fractionated radioimmunotherapy of non-Hodgkin’s lymphoma: A pilot study. Anticancer Res.; 1998; 18, pp. 2779-2788. [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/9713461]

56. Cu64-DOTA-trastuzumab PET and Markers Predicting Response to Neoadjuvant Trastuzumab + Pertuzum in HER2+ Breast Cancer. Available online: https://clinicaltrials.gov/search?term=NCT02827877 (accessed on 20 August 2023).

57. An Investigational Scan (64Cu-Labeled M5A Antibody) in Combination with SOC Chemotherapy and Radiotherapy in Locally Advanced Rectal Cancer. Available online: https://clinicaltrials.gov/search?term=NCT05245786 (accessed on 20 August 2023).

58. A Diagnostic Imaging Study of 64Cu-SARTATE Using PET on Patients with Known or Suspected Neuroendocrine Tumors (DISCO). Available online: https://clinicaltrials.gov/search?term=NCT04438304 (accessed on 20 August 2023).

59. Copper Cu 64 TP3805 PET in Detecting Cancer in Patients with Prostate Cancer Undergoing Surgery. Available online: https://clinicaltrials.gov/search?term=NCT02603965 (accessed on 15 August 2023).

60. Evaluation of a New Radiotracer (64Cu-DOTA-AE105) for Diagnosing Aggressive Cancer with Positron Emission Tomography. Available online: https://clinicaltrials.gov/search?term=NCT02139371 (accessed on 15 August 2023).

61. Positron Emission Tomography (PET) Imaging of Participants with Confirmed Prostate Cancer Using 64Cu-SAR-bisPSMA (PROPELLER) (PROPELLER). Available online: https://clinicaltrials.gov/search?term=NCT04839367 (accessed on 15 August 2023).

62. 64Cu-SAR-bisPSMA for Identification of Participants with Recurrence of Prostate Cancer (COBRA) (COBRA). Available online: https://clinicaltrials.gov/search?term=NCT05249127 (accessed on 15 August 2023).

63. Peptide Receptor Radionuclide Therapy Administered to Participants with Meningioma With 67Cu-SARTATE™. Available online: https://clinicaltrials.gov/search?term=NCT03936426 (accessed on 15 August 2023).

64. 67Cu-SARTATE™ Peptide Receptor Radionuclide Therapy Administered to Pediatric Patients with High-Risk, Relapsed, Refractory Neuroblastoma. Available online: https://clinicaltrials.gov/search?term=NCT04023331 (accessed on 15 August 2023).

65. 64Cu-SAR-bisPSMA and 67Cu-SAR-bisPSMA for Identification and Treatment of PSMA-Expressing Metastatic Castrate Resistant Prostate Cancer (SECuRE) (SECuRE). Available online: https://clinicaltrials.gov/search?term=NCT04868604 (accessed on 15 August 2023).

66. McCarthy, D.W.; Shefer, R.E.; Klinkowstein, R.E.; Bass, L.A.; Margeneau, W.H.; Cutler, C.S.; Anderson, C.J.; Welch, M.J. Efficient production of high specific activity 64Cu using a biomedical cyclotron. Nucl. Med. Biol.; 1997; 24, pp. 35-43. [DOI: https://dx.doi.org/10.1016/S0969-8051(96)00157-6]

67. Szelecsényi, F.; Steyn, G.; Dolley, S.; Kovács, Z.; Vermeulen, C.; Van der Walt, T. Investigation of the 68Zn (p, 2p) 67Cu nuclear reaction: New measurements up to 40 MeV and compilation up to 100 MeV. Nucl. Instrum. Methods Phys. Res. Sect. B Beam Interact. Mater. At.; 2009; 267, pp. 1877-1881. [DOI: https://dx.doi.org/10.1016/j.nimb.2009.03.097]

68. Mou, L.P.G.; Martini, P.; Pasquali, M. A Method and a Target for the Production of 67Cu 2019. Available online: https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2019220224 (accessed on 15 August 2023).

69. Mikuš, P.; Melník, M.; Forgácsová, A.; Krajčiová, D.; Havránek, E. Gallium compounds in nuclear medicine and oncology. Main Group Met. Chem.; 2014; 37, pp. 53-65. [DOI: https://dx.doi.org/10.1515/mgmc-2014-0009]

70. Amor-Coarasa, A.; Kelly, J.M.; Ponnala, S.; Nikolopoulou, A.; Williams, C., Jr.; Babich, J.W. ⁶⁶Ga: A Novelty or a Valuable Preclinical Screening Tool for the Design of Targeted Radiopharmaceuticals?. Molecules; 2018; 23, 2575. [DOI: https://dx.doi.org/10.3390/molecules23102575] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/30304795]

71. Bailey, D.L.; Sabanathan, D.; Aslani, A.; Campbell, D.H.; Walsh, B.J.; Lengkeek, N.A. RetroSPECT: Gallium-67 as a Long-Lived Imaging Agent for Theranostics. Asia Ocean J. Nucl. Med. Biol.; 2021; 9, 1. [DOI: https://dx.doi.org/10.22038/aojnmb.2020.51714.1355]

72. Rosenthall, L.; Kloiber, R.; Damtew, B.; Al-Majid, H. Sequential use of radiophosphate and radiogallium imaging in the differential diagnosis of bone, joint and soft tissue infection: Quantitative analysis. Diagn. Imaging; 1982; 51, pp. 249-258.

73. Hetherington, V.J. Technetium and combined gallium and technetium scans in the neurotrophic foot. J. Am. Podiatry Assoc.; 1982; 72, pp. 458-463. [DOI: https://dx.doi.org/10.7547/87507315-72-9-458]

74. Jhanwar, Y.S.; Straus, D.J. The Role of PET in Lymphoma. J. Nucl. Med.; 2006; 47, pp. 1326-1334.

75. Klech, H.; Köhn, H.; Huppmann, M.; Pohl, W. Thoracic imaging with gallium-67. Eur. J. Nucl. Med.; 1987; 13, (Suppl. S1), pp. S24-S36. [DOI: https://dx.doi.org/10.1007/BF00253288]

76. Fosburg, R.G.; Hopkins, G.B.; Kan, M.K. Evaluation of the mediastinum by gallium-67 scintigraphy in lung cancer. J. Thorac. Cardiovasc. Surg.; 1979; 77, pp. 76-82. [DOI: https://dx.doi.org/10.1016/S0022-5223(19)40991-4]

77. Graham, F.; Lord, M.; Froment, D.; Cardinal, H.; Bollée, G. The use of gallium-67 scintigraphy in the diagnosis of acute interstitial nephritis. Clin. Kidney J.; 2015; 9, pp. 76-81. [DOI: https://dx.doi.org/10.1093/ckj/sfv129]

78. International Atomic Energy Agency. Alternative Radionuclide Production with a Cyclotron; International Atomic Energy Agency: Vienna, Austria, 2021.

79. Banerjee, S.R.; Pomper, M.G. Clinical applications of Gallium-68. Appl. Radiat. Isot.; 2013; 76, pp. 2-13. [DOI: https://dx.doi.org/10.1016/j.apradiso.2013.01.039]

80. Fani, M.; André, J.P.; Maecke, H.R. 68Ga-PET: A powerful generator-based alternative to cyclotron-based PET radiopharmaceuticals. Contrast Media Mol. Imaging; 2008; 3, pp. 67-77. [DOI: https://dx.doi.org/10.1002/cmmi.232]

81. Zhernosekov, K.P.; Filosofov, D.V.; Baum, R.P.; Aschoff, P.; Bihl, H.; Razbash, A.A.; Jahn, M.; Jennewein, M.; Rösch, F. Processing of generator-produced 68Ga for medical application. J. Nucl. Med.; 2007; 48, pp. 1741-1748. [DOI: https://dx.doi.org/10.2967/jnumed.107.040378]

82. Yang, J.; Kan, Y.; Ge, B.H.; Yuan, L.; Li, C.; Zhao, W. Diagnostic role of Gallium-68 DOTATOC and Gallium-68 DOTATATE PET in patients with neuroendocrine tumors: A meta-analysis. Acta Radiol.; 2014; 55, pp. 389-398. [DOI: https://dx.doi.org/10.1177/0284185113496679]

83. Ambrosini, V.; Campana, D.; Bodei, L.; Nanni, C.; Castellucci, P.; Allegri, V.; Montini, G.C.; Tomassetti, P.; Paganelli, G.; Fanti, S. 68Ga-DOTANOC PET/CT clinical impact in patients with neuroendocrine tumors. J. Nucl. Med.; 2010; 51, pp. 669-673. [DOI: https://dx.doi.org/10.2967/jnumed.109.071712] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/20395323]

84. Verma, P.; Malhotra, G.; Goel, A.; Rakshit, S.; Chandak, A.; Chedda, R.; Banerjee, S.; Asopa, R.V. Differential Uptake of 68Ga-PSMA-HBED-CC (PSMA-11) in Low-Grade Versus High-Grade Gliomas in Treatment-Naive Patients. Clin. Nucl. Med.; 2019; 44, pp. e318-e322. [DOI: https://dx.doi.org/10.1097/RLU.0000000000002520] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/30829867]

85. Lenzo, N.P.; Meyrick, D.; Turner, J.H. Review of Gallium-68 PSMA PET/CT Imaging in the Management of Prostate Cancer. Diagnostics; 2018; 8, 16. [DOI: https://dx.doi.org/10.3390/diagnostics8010016]

86. Wang, G.; Zhou, M.; Zang, J.; Jiang, Y.; Chen, X.; Zhu, Z.; Chen, X. A pilot study of 68 Ga-PSMA-617 PET/CT imaging and 177Lu-EB-PSMA-617 radioligand therapy in patients with adenoid cystic carcinoma. EJNMMI Res.; 2022; 12, 52. [DOI: https://dx.doi.org/10.1186/s13550-022-00922-x] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/35984529]

87. Maffey-Steffan, J.; Scarpa, L.; Svirydenka, A.; Nilica, B.; Mair, C.; Buxbaum, S.; Bektic, J.; von Guggenberg, E.; Uprimny, C.; Horninger, W. et al. The ⁶⁸Ga/¹⁷⁷Lu-theragnostic concept in PSMA-targeting of metastatic castration-resistant prostate cancer: Impact of post-therapeutic whole-body scintigraphy in the follow-up. Eur. J. Nucl. Med. Mol. Imaging; 2020; 47, pp. 695-712. [DOI: https://dx.doi.org/10.1007/s00259-019-04583-2]

88. Ling, S.W.; de Blois, E.; Hooijman, E.; van der Veldt, A.; Brabander, T. Advances in 177Lu-PSMA and 225Ac-PSMA Radionuclide Therapy for Metastatic Castration-Resistant Prostate Cancer. Pharmaceutics; 2022; 14, 2166. [DOI: https://dx.doi.org/10.3390/pharmaceutics14102166]

89. Nelson, B.J.B.; Andersson, J.D.; Wuest, F.; Spreckelmeyer, S. Good practices for 68Ga radiopharmaceutical production. EJNMMI Radiopharm. Chem.; 2022; 7, 27. [DOI: https://dx.doi.org/10.1186/s41181-022-00180-1]

90. Lepareur, N. Cold Kit Labeling: The Future of ⁶⁸Ga Radiopharmaceuticals?. Front. Med.; 2022; 9, 812050. [DOI: https://dx.doi.org/10.3389/fmed.2022.812050]

91. Pandey, M.K.; Byrne, J.F.; Jiang, H.; Packard, A.B.; DeGrado, T.R. Cyclotron production of ⁶⁸Ga via the ⁶⁸Zn(p,n)⁶⁸Ga reaction in aqueous solution. Am. J. Nucl. Med. Mol. Imaging; 2014; 4, pp. 303-310.

92. Pandey, M.K.; Byrne, J.F.; Schlasner, K.N.; Schmit, N.R.; DeGrado, T.R. Cyclotron production of ⁶⁸Ga in a liquid target: Effects of solution composition and irradiation parameters. Nucl. Med. Biol.; 2019; 74, pp. 49-55. [DOI: https://dx.doi.org/10.1016/j.nucmedbio.2019.03.002]

93. Pandey, M.K.; DeGrado, T.R. Cyclotron Production of PET Radiometals in Liquid Targets: Aspects and Prospects. Curr. Radiopharm.; 2021; 14, pp. 325-339. [DOI: https://dx.doi.org/10.2174/18744729MTA5sMzgr4]

94. Mueller, D.; Schlitter, M.; Senftleben, S.; Proehl, M.; Fuchs, A.; Leshch, Y. First Results of Cyclotron Produced ⁶⁸Ga Using Solid Targets. J. Nucl. Med.; 2019; 60, 1163.

95. Lin, M.; Mukhopadhyay, U.; Waligorski, G.; Balatoni, J.; Gonzalez-Lepera, C. Production of Curie quantities of ⁶⁸Ga with a medical cyclotron via the ⁶⁸Zn(p,n)⁶⁸Ga reaction. J. Nucl. Med.; 2017; 58, 336. [DOI: https://dx.doi.org/10.1016/j.apradiso.2017.12.010] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/29272820]

96. Alnahwi, A.H.; Tremblay, S.; Ait-Mohand, S.; Beaudoin, J.F.; Guérin, B. Automated radiosynthesis of ⁶⁸Ga for large-scale routine production using ⁶⁸Zn pressed target. Appl. Radiat. Isot.; 2020; 156, 109014. [DOI: https://dx.doi.org/10.1016/j.apradiso.2019.109014] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/32056692]

97. Andrade Martins, P.d.; Osso, J.A. Thermal diffusion of 67Ga from irradiated Zn targets. Appl. Radiat. Isot.; 2013; 82, pp. 279-282. [DOI: https://dx.doi.org/10.1016/j.apradiso.2013.08.012]

98. Ga-68 PSMA Ligand: A Radiopharmaceutical for Localization of Prostate Cancer. Available online: https://clinicaltrials.gov/search?term=NCT03207139 (accessed on 15 August 2023).

99. 68Ga PSMA PET/MRI for Hepatocellular Carcinoma. Available online: https://clinicaltrials.gov/search?term=NCT03982407 (accessed on 15 August 2023).

100. A Phase 1/2 Clinical Trial to Evaluate the Safety, Tolerability, Dosimetry, and Anti-tumor Activity of Ga-68-NGUL/Lu-177-DGUL in Patients with Metastatic Castration-resistant Prostate Cancer (mCRPC) Refractory to Standard Therapy. Available online: https://clinicaltrials.gov/search?term=NCT05547061 (accessed on 15 August 2023).

101. Direct Comparison of Ga-68-DOTATATE and Ga-68-DOTATOC. Available online: https://clinicaltrials.gov/search?term=NCT04298541 (accessed on 15 August 2023).

102. Impact of Ga-68 DOTATOC PET-CT Imaging in Management of Neuroendocrine Tumors. Available online: https://clinicaltrials.gov/search?term=NCT02441062 (accessed on 15 August 2023).

103. 68Ga-FAPi-46 PET/CT Scan in Imaging Patients with Sarcoma. Available online: https://clinicaltrials.gov/search?term=NCT04457258 (accessed on 15 August 2023).

104. Hegi-Johnson, F.; Rudd, S.; Hicks, R.J.; De Ruysscher, D.; Trapani, J.A.; John, T.; Donnelly, P.; Blyth, B.; Hanna, G.; Everitt, S. et al. Imaging immunity in patients with cancer using positron emission tomography. NPJ Precis. Oncol.; 2022; 6, 24. [DOI: https://dx.doi.org/10.1038/s41698-022-00263-x] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/35393508]

105. Deri, M.A.; Zeglis, B.M.; Francesconi, L.C.; Lewis, J.S. PET imaging with ⁸⁹Zr: From radiochemistry to the clinic. Nucl Med Biol; 2013; 40, pp. 3-14. [DOI: https://dx.doi.org/10.1016/j.nucmedbio.2012.08.004]

106. Börjesson, P.K.; Jauw, Y.W.; Boellaard, R.; de Bree, R.; Comans, E.F.; Roos, J.C.; Castelijns, J.A.; Vosjan, M.J.; Kummer, J.A.; Leemans, C.R. et al. Performance of immuno-positron emission tomography with zirconium-89-labeled chimeric monoclonal antibody U36 in the detection of lymph node metastases in head and neck cancer patients. Clin. Cancer Res.; 2006; 12, pp. 2133-2140. [DOI: https://dx.doi.org/10.1158/1078-0432.CCR-05-2137]

107. Mullard, A. FDA approves 100th monoclonal antibody product. Nat. Rev. Drug Discov.; 2021; 20, pp. 491-495. [DOI: https://dx.doi.org/10.1038/d41573-021-00079-7]

108. Jauw, Y.W.; Menke-van der Houven van Oordt, C.W.; Hoekstra, O.S.; Hendrikse, N.H.; Vugts, D.J.; Zijlstra, J.M.; Huisman, M.C.; van Dongen, G.A. Immuno-Positron Emission Tomography with Zirconium-89-Labeled Monoclonal Antibodies in Oncology: What Can We Learn from Initial Clinical Trials?. Front. Pharmacol.; 2016; 7, 131. [DOI: https://dx.doi.org/10.3389/fphar.2016.00131]

109. Dijkers, E.C.; Oude Munnink, T.H.; Kosterink, J.G.; Brouwers, A.H.; Jager, P.L.; de Jong, J.R.; van Dongen, G.A.; Schröder, C.P.; Lub-de Hooge, M.N.; de Vries, E.G. Biodistribution of 89Zr-trastuzumab and PET imaging of HER2-positive lesions in patients with metastatic breast cancer. Clin. Pharmacol. Ther.; 2010; 87, pp. 586-592. [DOI: https://dx.doi.org/10.1038/clpt.2010.12] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/20357763]

110. Ulaner, G.A.; Lyashchenko, S.K.; Riedl, C.; Ruan, S.; Zanzonico, P.B.; Lake, D.; Jhaveri, K.; Zeglis, B.; Lewis, J.S.; O’Donoghue, J.A. First-in-Human Human Epidermal Growth Factor Receptor 2-Targeted Imaging Using ⁸⁹Zr-Pertuzumab PET/CT: Dosimetry and Clinical Application in Patients with Breast Cancer. J. Nucl. Med.; 2018; 59, pp. 900-906. [DOI: https://dx.doi.org/10.2967/jnumed.117.202010] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/29146695]

111. Bahce, I.; Huisman, M.C.; Verwer, E.E.; Ooijevaar, R.; Boutkourt, F.; Vugts, D.J.; van Dongen, G.A.M.S.; Boellaard, R.; Smit, E.F. Pilot study of ⁸⁹Zr-bevacizumab positron emission tomography in patients with advanced non-small cell lung cancer. EJNMMI Res.; 2014; 4, 35. [DOI: https://dx.doi.org/10.1186/s13550-014-0035-5] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/26055936]

112. Jauw, Y.W.; Zijlstra, J.M.; de Jong, D.; Vugts, D.J.; Zweegman, S.; Hoekstra, O.S.; van Dongen, G.A.; Huisman, M.C. Performance of 89Zr-Labeled-Rituximab-PET as an Imaging Biomarker to Assess CD20 Targeting: A Pilot Study in Patients with Relapsed/Refractory Diffuse Large B Cell Lymphoma. PLoS ONE; 2017; 12, e0169828. [DOI: https://dx.doi.org/10.1371/journal.pone.0169828] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/28060891]

113. Menke-van der Houven van Oordt, C.W.; Gootjes, E.C.; Huisman, M.C.; Vugts, D.J.; Roth, C.; Luik, A.M.; Mulder, E.R.; Schuit, R.C.; Boellaard, R.; Hoekstra, O.S. et al. 89Zr-cetuximab PET imaging in patients with advanced colorectal cancer. Oncotarget; 2015; 6, pp. 30384-30393. [DOI: https://dx.doi.org/10.18632/oncotarget.4672]

114. Pilot Study of Zirconium-89 Bevacizumab Positron Emission Tomography for Imaging Angiogenesis in Patients with Inflammatory Breast Carcinoma Receiving Preoperative Chemotherapy. Available online: https://clinicaltrials.gov/search?term=NCT01894451 (accessed on 15 August 2023).

115. Chames, P.; Van Regenmortel, M.; Weiss, E.; Baty, D. Therapeutic antibodies: Successes, limitations and hopes for the future. Br. J. Pharmacol.; 2009; 157, pp. 220-233. [DOI: https://dx.doi.org/10.1111/j.1476-5381.2009.00190.x] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/19459844]

116. Vlachostergios, P.J.; Niaz, M.J.; Thomas, C.; Christos, P.J.; Osborne, J.R.; Margolis, D.J.A.; Khani, F.; Bander, N.H.; Scherr, D.S.; Tagawa, S.T. Pilot study of the diagnostic utility of ⁸⁹ Zr-df-IAB2M and ⁶⁸ Ga-PSMA-11 PET imaging and multiparametric MRI in localized prostate cancer. Prostate; 2022; 82, pp. 483-492. [DOI: https://dx.doi.org/10.1002/pros.24294]

117. Matsuda, M.; Ishikawa, E.; Yamamoto, T.; Hatano, K.; Joraku, A.; Iizumi, Y.; Masuda, Y.; Nishiyama, H.; Matsumura, A. Potential use of prostate specific membrane antigen (PSMA) for detecting the tumor neovasculature of brain tumors by PET imaging with ⁸⁹Zr-Df-IAB2M anti-PSMA minibody. J. Neurooncol.; 2018; 138, pp. 581-589. [DOI: https://dx.doi.org/10.1007/s11060-018-2825-5]

118. Burley, T.A.; Da Pieve, C.; Martins, C.D.; Ciobota, D.M.; Allott, L.; Oyen, W.J.G.; Harrington, K.J.; Smith, G.; Kramer-Marek, G. Affibody-Based PET Imaging to Guide EGFR-Targeted Cancer Therapy in Head and Neck Squamous Cell Cancer Models. J. Nucl. Med.; 2019; 60, pp. 353-361. [DOI: https://dx.doi.org/10.2967/jnumed.118.216069]

119. Xu, Y.; Wang, L.; Pan, D.; Yan, J.; Wang, X.; Yang, R.; Li, M.; Liu, Y.; Yang, M. Synthesis of a novel ⁸⁹Zr-labeled HER2 affibody and its application study in tumor PET imaging. EJNMMI Res.; 2020; 10, 58. [DOI: https://dx.doi.org/10.1186/s13550-020-00649-7]

120. Weist, M.R.; Starr, R.; Aguilar, B.; Chea, J.; Miles, J.K.; Poku, E.; Gerdts, E.; Yang, X.; Priceman, S.J.; Forman, S.J. et al. PET of Adoptively Transferred Chimeric Antigen Receptor T Cells with ⁸⁹Zr-Oxine. J. Nucl. Med.; 2018; 59, pp. 1531-1537. [DOI: https://dx.doi.org/10.2967/jnumed.117.206714] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/29728514]

121. Yusufi, N.; Mall, S.; Bianchi, H.O.; Steiger, K.; Reder, S.; Klar, R.; Audehm, S.; Mustafa, M.; Nekolla, S.; Peschel, C. et al. In-depth Characterization of a TCR-specific Tracer for Sensitive Detection of Tumor-directed Transgenic T Cells by Immuno-PET. Theranostics; 2017; 7, pp. 2402-2416. [DOI: https://dx.doi.org/10.7150/thno.17994]

122. Bansal, A.; Pandey, M.K.; Demirhan, Y.E.; Nesbitt, J.J.; Crespo-Diaz, R.J.; Terzic, A.; Behfar, A.; DeGrado, T.R. Novel 89Zr cell labeling approach for PET-based cell trafficking studies. EJNMMI Res.; 2015; 5, 19. [DOI: https://dx.doi.org/10.1186/s13550-015-0098-y] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/25918673]

123. Yang, B.; Brahmbhatt, A.; Nieves Torres, E.; Thielen, B.; McCall, D.L.; Engel, S.; Bansal, A.; Pandey, M.K.; Dietz, A.B.; Leof, E.B. et al. Tracking and Therapeutic Value of Human Adipose Tissue-derived Mesenchymal Stem Cell Transplantation in Reducing Venous Neointimal Hyperplasia Associated with Arteriovenous Fistula. Radiology; 2016; 279, pp. 513-522. [DOI: https://dx.doi.org/10.1148/radiol.2015150947]

124. Bansal, A.; Pandey, M.K.; Yamada, S.; Goyal, R.; Schmit, N.R.; Jeon, R.; Nesbitt, J.J.; Witt, T.A.; Singh, R.D.; Gunderson, T.M. et al. [⁸⁹Zr]Zr-DBN labeled cardiopoietic stem cells proficient for heart failure. Nucl. Med. Biol.; 2020; 90, pp. 23-30. [DOI: https://dx.doi.org/10.1016/j.nucmedbio.2020.09.001] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/32957056]

125. Bansal, A.; Sharma, S.; Klasen, B.; Rösch, F.; Pandey, M.K. Evaluation of different 89Zr-labeled synthons for direct labeling and tracking of white blood cells and stem cells in healthy athymic mice. Sci. Rep.; 2022; 12, 15646. [DOI: https://dx.doi.org/10.1038/s41598-022-19953-4]

126. Pandey, M.K.; Bansal, A.; Ellinghuysen, J.R.; Vail, D.J.; Berg, H.M.; DeGrado, T.R. A new solid target design for the production of ⁸⁹Zr and radiosynthesis of high molar activity [⁸⁹Zr]Zr-DBN. Am. J. Nucl. Med. Mol. Imaging; 2022; 12, pp. 15-24. [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/35295887]

127. Holland, J.P.; Sheh, Y.; Lewis, J.S. Standardized methods for the production of high specific-activity zirconium-89. Nucl. Med. Biol.; 2009; 36, pp. 729-739. [DOI: https://dx.doi.org/10.1016/j.nucmedbio.2009.05.007]

128. Pandey, M.; Engelbrecht, H.; Byrne, J.; Packard, A.; Degrado, T. Production of 89Zr via the 89Y(p,n)89Zr reaction in aqueous solution: Effect of solution composition on in-target chemistry. Nucl. Med. Biol.; 2014; 41, pp. 309-316. [DOI: https://dx.doi.org/10.1016/j.nucmedbio.2014.01.006] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/24607433]

129. Kasbollah, A.; Eu, P.; Cowell, S.; Deb, P. Review on Production of ⁸⁹Zr in a Medical Cyclotron for PET Radiopharmaceuticals. J. Nucl. Med. Technol.; 2013; 41, pp. 35-41. [DOI: https://dx.doi.org/10.2967/jnmt.112.111377]

130. Queern, S.L.; Aweda, T.A.; Massicano, A.V.F.; Clanton, N.A.; El Sayed, R.; Sader, J.A.; Zyuzin, A.; Lapi, S.E. Production of Zr-89 using sputtered yttrium coin targets ⁸⁹Zr using sputtered yttrium coin targets. Nucl. Med. Biol.; 2017; 50, pp. 11-16. [DOI: https://dx.doi.org/10.1016/j.nucmedbio.2017.03.004]

131. Larenkov, A.; Bubenschikov, V.; Makichyan, A.; Zhukova, M.; Krasnoperova, A.; Kodina, G. Preparation of Zirconium-89 Solutions for Radiopharmaceutical Purposes: Interrelation Between Formulation, Radiochemical Purity, Stability and Biodistribution. Molecules; 2019; 24, 1534. [DOI: https://dx.doi.org/10.3390/molecules24081534] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/31003494]

132. Alnahwi, A.H.; Tremblay, S.; Guérin, B. Comparative Study with ⁸⁹Y-foil and ⁸⁹Y-pressed Targets for the Production of ⁸⁹Zr. Appl. Sci.; 2018; 8, 1579. [DOI: https://dx.doi.org/10.3390/app8091579]

133. Wooten, A.L.; Madrid, E.; Schweitzer, G.D.; Lawrence, L.A.; Mebrahtu, E.; Lewis, B.C.; Lapi, S.E. Routine Production of ⁸⁹Zr Using an Automated Module. Appl. Sci.; 2013; 3, pp. 593-613. [DOI: https://dx.doi.org/10.3390/app3030593]

134. Pandey, M.K.; Bansal, A.; Engelbrecht, H.P.; Byrne, J.F.; Packard, A.B.; DeGrado, T.R. Improved production and processing of ⁸⁹Zr using a solution target. Nucl. Med. Biol.; 2016; 43, pp. 97-100. [DOI: https://dx.doi.org/10.1016/j.nucmedbio.2015.09.007] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/26471714]

135. Lapi, S.E. Production of Positron Emitting Radiometals: Cu-64, Y-86, Zr-89; Final Report Washington University: St. Louis, MO, USA, 2014; [DOI: https://dx.doi.org/10.2172/1304997]

136. Severin, G.W.; Engle, J.W.; Barnhart, T.E.; Nickles, R.J. 89Zr radiochemistry for positron emission tomography. Med. Chem.; 2011; 7, pp. 389-394. [DOI: https://dx.doi.org/10.2174/157340611796799186] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/21711221]

137. Clinical and Molecular Correlates of Positron Emission Tomography (PET) with 89Zr-DFO-huJ591 in Metastatic Prostate Cancer. Available online: https://clinicaltrials.gov/search?term=NCT01543659 (accessed on 15 August 2023).

138. Comparison of PET With [89Zr]-Df-IAb2M and [111In]-Capromab Pendetide in the Detection of Prostate Cancer Pre-Prostatectomy. Available online: https://clinicaltrials.gov/search?term=NCT02349022 (accessed on 15 August 2023).

139. Study of Zirconium Zr 89 Crefmirlimab Berdoxam PET/CT in Subjects with Advanced or Metastatic Malignancies. Available online: https://clinicaltrials.gov/search?term=NCT05013099 (accessed on 15 August 2023).

140. ⁸⁹Zr-Df-IAB22M2C PET/CT in Patients with Selected Solid Malignancies or Hodgkin’s Lymphoma. Available online: https://clinicaltrials.gov/search?term=NCT03107663 (accessed on 15 August 2023).

141. ⁸⁹Zr-Df-IAB22M2C (CD8 PET Tracer) for PET/CT in Patients with Metastatic Solid Tumors. Available online: https://clinicaltrials.gov/search?term=NCT03802123 (accessed on 15 August 2023).

142. First-in- Human Imaging of Multiple Myeloma Using 89Zr-DFO-daratumumab, a CD38-targeting Monoclonal Antibody. Available online: https://clinicaltrials.gov/search?term=NCT03665155 (accessed on 15 August 2023).

143. Pilot Imaging Study With 89Zr-Trastuzumab in HER2-positive Metastatic Breast Cancer Patients. Available online: https://clinicaltrials.gov/search?term=NCT01420146 (accessed on 15 August 2023).

144. Testing a New Imaging Agent to Identify Cancer. Available online: https://clinicaltrials.gov/search?term=NCT04692831 (accessed on 15 August 2023).

145. Panitumumab-IRDye800 and 89Zr-Panitumumab in Identifying Metastatic Lymph Nodes in Patients with Squamous Cell Head and Neck Cancer. Available online: https://clinicaltrials.gov/search?term=NCT03733210%20 (accessed on 15 August 2023).

146. Non-invasive Imaging of Cetuximab-Zr. 89 Uptake Wit PET: A Phase I Trial in Stage IV Cancer Patients. Available online: https://clinicaltrials.gov/search?term=NCT00691548 (accessed on 15 August 2023).

147. 89Zr-TLX250 for PET/CT Imaging of ccRCC—ZIRCON Study. Available online: https://clinicaltrials.gov/search?term=NCT03849118 (accessed on 15 August 2023).

148. Imaging Tumor-infiltrating T-cells in Non-small Cell Lung Cancer. Available online: https://clinicaltrials.gov/search?term=NCT03853187 (accessed on 15 August 2023).

149. 89Zr-DFO-Atezolizumab ImmunoPET/CT in Patients with Locally Advanced or Metastatic Renal Cell Carcinoma. Available online: https://clinicaltrials.gov/search?term=NCT04006522 (accessed on 15 August 2023).

150. Müller, C.; Domnanich, K.A.; Umbricht, C.A.; van der Meulen, N.P. Scandium and terbium radionuclides for radiotheranostics: Current state of development towards clinical application. Br. J. Radiol.; 2018; 91, 20180074. [DOI: https://dx.doi.org/10.1259/bjr.20180074]

151. Rosar, F.; Buchholz, H.-G.; Michels, S.; Roesch, F.; Piel, M.; Miederer, M.; Reuss, S.; Schreckenberger, M. A performance comparison of Sc-44 to Ga-68 using preclinical PET scanners. J. Nucl. Med.; 2018; 59, 1776.

152. Ferguson, S.; Jans, H.S.; Wuest, M.; Riauka, T.; Wuest, F. Comparison of scandium-44 g with other PET radionuclides in pre-clinical PET phantom imaging. EJNMMI Phys.; 2019; 6, 23. [DOI: https://dx.doi.org/10.1186/s40658-019-0260-0]

153. Singh, A.; van der Meulen, N.P.; Müller, C.; Klette, I.; Kulkarni, H.R.; Türler, A.; Schibli, R.; Baum, R.P. First-in-Human PET/CT Imaging of Metastatic Neuroendocrine Neoplasms with Cyclotron-Produced ⁴⁴Sc-DOTATOC: A Proof-of-Concept Study. Cancer Biother. Radiopharm.; 2017; 32, pp. 124-132. [DOI: https://dx.doi.org/10.1089/cbr.2016.2173] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/28514206]

154. Eppard, E.; de la Fuente, A.; Benešová, M.; Khawar, A.; Bundschuh, R.A.; Gärtner, F.C.; Kreppel, B.; Kopka, K.; Essler, M.; Rösch, F. Clinical Translation and First In-Human Use of [⁴⁴Sc]Sc-PSMA-617 for PET Imaging of Metastasized Castrate-Resistant Prostate Cancer. Theranostics; 2017; 7, pp. 4359-4369. [DOI: https://dx.doi.org/10.7150/thno.20586] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/29158832]

155. Müller, C.; Bunka, M.; Haller, S.; Köster, U.; Groehn, V.; Bernhardt, P.; van der Meulen, N.; Türler, A.; Schibli, R. Promising prospects for 44Sc-/47Sc-based theragnostics: Application of 47Sc for radionuclide tumor therapy in mice. J. Nucl. Med.; 2014; 55, pp. 1658-1664. [DOI: https://dx.doi.org/10.2967/jnumed.114.141614]

156. Siwowska, K.; Guzik, P.; Domnanich, K.A.; Monné Rodríguez, J.M.; Bernhardt, P.; Ponsard, B.; Hasler, R.; Borgna, F.; Schibli, R.; Köster, U. et al. Therapeutic Potential of ⁴⁷Sc in Comparison to ¹⁷⁷Lu and ⁹⁰Y: Preclinical Investigations. Pharmaceutics; 2019; 11, 424. [DOI: https://dx.doi.org/10.3390/pharmaceutics11080424] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/31434360]

157. Filosofov, D.V.; Loktionova, N.S.; Rösch, F. A 44Ti/44Sc radionuclide generator for potential application of 44Sc-based PET-radiopharmaceuticals. rca Radiochim. Acta; 2010; 98, pp. 149-156. [DOI: https://dx.doi.org/10.1524/ract.2010.1701]

158. Van der Meulen, N.P.; Bunka, M.; Domnanich, K.A.; Müller, C.; Haller, S.; Vermeulen, C.; Türler, A.; Schibli, R. Cyclotron production of ⁴⁴Sc: From bench to bedside. Nucl. Med. Biol.; 2015; 42, pp. 745-751. [DOI: https://dx.doi.org/10.1016/j.nucmedbio.2015.05.005]

159. Szkliniarz, K.; Sitarz, M.; Walczak, R.; Jastrzębski, J.; Bilewicz, A.; Choiński, J.; Jakubowski, A.; Majkowska, A.; Stolarz, A.; Trzcińska, A. et al. Production of medical Sc radioisotopes with an alpha particle beam. Appl. Radiat. Isot.; 2016; 118, pp. 182-189. [DOI: https://dx.doi.org/10.1016/j.apradiso.2016.07.001] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/27642728]

160. Domnanich, K.A.; Eichler, R.; Müller, C.; Jordi, S.; Yakusheva, V.; Braccini, S.; Behe, M.; Schibli, R.; Türler, A.; van der Meulen, N.P. Production and separation of 43Sc for radiopharmaceutical purposes. EJNMMI Radiopharm. Chem.; 2017; 2, 14. [DOI: https://dx.doi.org/10.1186/s41181-017-0033-9]

161. Wibowo, F.A.; Kambali, I. Calculated cyclotron-based scandium-47 production yields via ⁴⁸Ca(p, 2n)⁴⁷Sc nuclear reaction. J. Phys. Conf. Ser.; 2021; 1816, 012099. [DOI: https://dx.doi.org/10.1088/1742-6596/1816/1/012099]

162. Gizawy, M.A.; Mohamed, N.M.A.; Aydia, M.I.; Soliman, M.A.; Shamsel-Din, H.A. Feasibility study on production of Sc-47 from neutron irradiated Ca target for cancer theranostics applications. Radiochim. Acta; 2020; 108, pp. 207-215. [DOI: https://dx.doi.org/10.1515/ract-2018-3070]

163. Rotsch, D.A.; Brown, M.A.; Nolen, J.A.; Brossard, T.; Henning, W.F.; Chemerisov, S.D.; Gromov, R.G.; Greene, J. Electron linear accelerator production and purification of scandium-47 from titanium dioxide targets. Appl. Radiat. Isot.; 2018; 131, pp. 77-82. [DOI: https://dx.doi.org/10.1016/j.apradiso.2017.11.007]

164. Mamtimin, M.; Harmon, F.; Starovoitova, V.N. Sc-47 production from titanium targets using electron linacs. Appl. Radiat. Isot.; 2015; 102, pp. 1-4. [DOI: https://dx.doi.org/10.1016/j.apradiso.2015.04.012]

165. Terbium: A New ‘Swiss Army Knife’ for Nuclear Medicine. Available online: https://cerncourier.com/a/terbium-a-new-swiss-army-knife-for-nuclear-medicine/ (accessed on 15 August 2023).

166. Naskar, N.; Lahiri, S. Theranostic Terbium Radioisotopes: Challenges in Production for Clinical Application. Front. Med.; 2021; 8, 675014. [DOI: https://dx.doi.org/10.3389/fmed.2021.675014] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/34136508]

167. Müller, C.; Zhernosekov, K.; Köster, U.; Johnston, K.; Dorrer, H.; Hohn, A.; van der Walt, N.T.; Türler, A.; Schibli, R. A unique matched quadruplet of terbium radioisotopes for PET and SPECT and for α- and β- radionuclide therapy: An in vivo proof-of-concept study with a new receptor-targeted folate derivative. J. Nucl. Med.; 2012; 53, pp. 1951-1959. [DOI: https://dx.doi.org/10.2967/jnumed.112.107540]

168. Müller, C.; Vermeulen, C.; Köster, U.; Johnston, K.; Türler, A.; Schibli, R.; van der Meulen, N.P. Alpha-PET with terbium-149: Evidence and perspectives for radiotheragnostics. EJNMMI Radiopharm. Chem.; 2016; 1, 5. [DOI: https://dx.doi.org/10.1186/s41181-016-0008-2] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/29564382]

169. Beyer, G.J.; Miederer, M.; Vranjes-Durić, S.; Comor, J.J.; Künzi, G.; Hartley, O.; Senekowitsch-Schmidtke, R.; Soloviev, D.; Buchegger, F. Targeted alpha therapy in vivo: Direct evidence for single cancer cell kill using 149Tb-rituximab. Eur. J. Nucl. Med. Mol. Imaging; 2004; 31, pp. 547-554. [DOI: https://dx.doi.org/10.1007/s00259-003-1413-9]

170. Baum, R.P.; Singh, A.; Benešová, M.; Vermeulen, C.; Gnesin, S.; Köster, U.; Johnston, K.; Müller, D.; Senftleben, S.; Kulkarni, H.R. et al. Clinical evaluation of the radiolanthanide terbium-152: First-in-human PET/CT with ¹⁵²Tb-DOTATOC. Dalton Trans.; 2017; 46, pp. 14638-14646. [DOI: https://dx.doi.org/10.1039/C7DT01936J] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/28825750]

171. Beyer, G.J.; Čomor, J.J.; Daković, M.; Soloviev, D.; Tamburella, C.; Hagebø, E.; Allan, B.; Dmitriev, S.N.; Zaitseva, N.G.; Collaboration, I. Production routes of the alpha emitting 149Tb for medical application. Radiochim. Acta; 2002; 90, pp. 247-252. [DOI: https://dx.doi.org/10.1524/ract.2002.90.5_2002.247]

172. Umbricht, C.A.; Köster, U.; Bernhardt, P.; Gracheva, N.; Johnston, K.; Schibli, R.; van der Meulen, N.P.; Müller, C. Alpha-PET for Prostate Cancer: Preclinical investigation using 149Tb-PSMA-617. Sci. Rep.; 2019; 9, 17800. [DOI: https://dx.doi.org/10.1038/s41598-019-54150-w]

173. Müller, C.; Fischer, E.; Behe, M.; Köster, U.; Dorrer, H.; Reber, J.; Haller, S.; Cohrs, S.; Blanc, A.; Grünberg, J. et al. Future prospects for SPECT imaging using the radiolanthanide terbium-155—Production and preclinical evaluation in tumor-bearing mice. Nucl. Med. Biol.; 2014; 41, (Suppl. S1), pp. e58-e65. [DOI: https://dx.doi.org/10.1016/j.nucmedbio.2013.11.002]

174. Haller, S.; Pellegrini, G.; Vermeulen, C.; van der Meulen, N.P.; Köster, U.; Bernhardt, P.; Schibli, R.; Müller, C. Contribution of Auger/conversion electrons to renal side effects after radionuclide therapy: Preclinical comparison of ¹⁶¹Tb-folate and ¹⁷⁷Lu-folate. EJNMMI Res.; 2016; 6, 13. [DOI: https://dx.doi.org/10.1186/s13550-016-0171-1]

175. Grünberg, J.; Lindenblatt, D.; Dorrer, H.; Cohrs, S.; Zhernosekov, K.; Köster, U.; Türler, A.; Fischer, E.; Schibli, R. Anti-L1CAM radioimmunotherapy is more effective with the radiolanthanide terbium-161 compared to lutetium-177 in an ovarian cancer model. Eur. J. Nucl. Med. Mol. Imaging; 2014; 41, pp. 1907-1915. [DOI: https://dx.doi.org/10.1007/s00259-014-2798-3] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/24859811]

176. Müller, C.; Reber, J.; Haller, S.; Dorrer, H.; Bernhardt, P.; Zhernosekov, K.; Türler, A.; Schibli, R. Direct in vitro and in vivo comparison of ¹⁶¹Tb and ¹⁷⁷Lu using a tumour-targeting folate conjugate. Eur. J. Nucl. Med. Mol. Imaging; 2014; 41, pp. 476-485. [DOI: https://dx.doi.org/10.1007/s00259-013-2563-z]

177. Hindié, E.; Zanotti-Fregonara, P.; Quinto, M.A.; Morgat, C.; Champion, C. Dose Deposits from ⁹⁰Y, ¹⁷⁷Lu, ¹¹¹In, and ¹⁶¹Tb in Micrometastases of Various Sizes: Implications for Radiopharmaceutical Therapy. J. Nucl. Med.; 2016; 57, pp. 759-764. [DOI: https://dx.doi.org/10.2967/jnumed.115.170423] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/26912441]

178. Baum, R.P.; Singh, A.; Kulkarni, H.R.; Bernhardt, P.; Rydén, T.; Schuchardt, C.; Gracheva, N.; Grundler, P.V.; Köster, U.; Müller, D. et al. First-in-Humans Application of ¹⁶¹Tb: A Feasibility Study Using ¹⁶¹Tb-DOTATOC. J. Nucl. Med.; 2021; 62, pp. 1391-1397. [DOI: https://dx.doi.org/10.2967/jnumed.120.258376] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/33547209]

179. Combined Beta- Plus Auger Electron Therapy Using a Novel Somatostatin Receptor Subtype 2 Antagonist Labelled with Terbium-161 (161Tb-DOTA-LM3). Available online: https://clinicaltrials.gov/search?term=NCT05359146 (accessed on 15 August 2023).

180. Evaluation of Radioligand Treatment in Men with Metastatic Castration-Resistant Prostate Cancer With [161Tb]Tb-PSMA-I&T. Available online: https://clinicaltrials.gov/search?term=NCT05521412 (accessed on 15 August 2023).

181. Lehenberger, S.; Barkhausen, C.; Cohrs, S.; Fischer, E.; Grünberg, J.; Hohn, A.; Köster, U.; Schibli, R.; Türler, A.; Zhernosekov, K. The low-energy β(-) and electron emitter ¹⁶¹Tb as an alternative to ¹⁷⁷Lu for targeted radionuclide therapy. Nucl. Med. Biol.; 2011; 38, pp. 917-924. [DOI: https://dx.doi.org/10.1016/j.nucmedbio.2011.02.007]

182. Firth, G.; Yu, Z.; Bartnicka, J.J.; Parker, D.; Kim, J.; Sunassee, K.; Greenwood, H.E.; Al-Salamee, F.; Jauregui-Osoro, M.; Di Pietro, A. et al. Imaging zinc trafficking in vivo by positron emission tomography with zinc-62. Metallomics; 2022; 14, mfac076. [DOI: https://dx.doi.org/10.1093/mtomcs/mfac076]

183. Fujibayashi, Y.; Saji, H.; Kawai, K.; Unuma, Y.; Miyata, S.; Okuno, T.; Hosotani, R.; Inoue, K.; Adachi, H.; Horiuchi, K. et al. A radiopharmaceutical for pancreatic exocrine functional diagnosis: 62Zn-EDDA metabolism in pancreas. Int. J. Nucl. Med. Biol.; 1986; 12, pp. 447-451. [DOI: https://dx.doi.org/10.1016/S0047-0740(86)80006-7] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/3086247]

184. DeGrado, T.R.; Pandey, M.K.; Byrne, J.F.; Engelbrecht, H.P.; Jiang, H.; Packard, A.B.; Thomas, K.A.; Jacobson, M.S.; Curran, G.L.; Lowe, V.J. Preparation and preliminary evaluation of 63Zn-zinc citrate as a novel PET imaging biomarker for zinc. J. Nucl. Med.; 2014; 55, pp. 1348-1354. [DOI: https://dx.doi.org/10.2967/jnumed.114.141218]

185. DeGrado, T.R.; Kemp, B.J.; Pandey, M.K.; Jiang, H.; Gunderson, T.M.; Linscheid, L.R.; Woodwick, A.R.; McConnell, D.M.; Fletcher, J.G.; Johnson, G.B. et al. First PET Imaging Studies With 63Zn-Zinc Citrate in Healthy Human Participants and Patients with Alzheimer Disease. Mol. Imaging; 2016; 15, 1536012116673793. [DOI: https://dx.doi.org/10.1177/1536012116673793]

186. World Nuclear Association. Radioisotopes in Medicine. Available online: http://www.world-nuclear.org/information-library/non-power-nuclear-applications/radioisotopes-research/radioisotopes-in-medicine.aspx (accessed on 15 August 2023).

187. Boschi, A.; Uccelli, L.; Marvelli, L.; Cittanti, C.; Giganti, M.; Martini, P. Technetium-99m Radiopharmaceuticals for Ideal Myocardial Perfusion Imaging: Lost and Found Opportunities. Molecules; 2022; 27, 1188. [DOI: https://dx.doi.org/10.3390/molecules27041188]

188. Duatti, A. Review on ^99mTc radiopharmaceuticals with emphasis on new advancements. Nucl. Med. Biol.; 2021; 92, pp. 202-216. [DOI: https://dx.doi.org/10.1016/j.nucmedbio.2020.05.005]

189. Boschi, A.; Uccelli, L.; Martini, P. A Picture of Modern Tc-99m Radiopharmaceuticals: Production, Chemistry, and Applications in Molecular Imaging. Appl. Sci.; 2019; 9, 2526. [DOI: https://dx.doi.org/10.3390/app9122526]

190. Artiko, V.; Sobic-Saranovic, D.; Pavlovic, S.; Petrovic, M.; Zuvela, M.; Antic, A.; Matic, S.; Odalovic, S.; Petrovic, N.; Milovanovic, A. et al. The clinical value of scintigraphy of neuroendocrine tumors using ^9mTc-HYNIC-TOC. J. BUON; 2012; 17, pp. 537-542. [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/23033296]

191. Fallahi, B.; Manafi-Farid, R.; Eftekhari, M.; Fard-Esfahani, A.; Emami-Ardekani, A.; Geramifar, P.; Akhlaghi, M.; Hashemi Taheri, A.P.; Beiki, D. Diagnostic efficiency of ⁶⁸Ga-DOTATATE PET/CT as compared to 99mTc-Octreotide SPECT/CT and conventional morphologic modalities in neuroendocrine tumors. Asia Ocean J. Nucl. Med. Biol.; 2019; 7, pp. 129-140. [DOI: https://dx.doi.org/10.22038/aojnmb.2019.39392.1263]

192. Slomka, P.; Hung, G.U.; Germano, G.; Berman, D.S. Novel SPECT Technologies and Approaches in Cardiac Imaging. Cardiovasc. Innov. Appl.; 2016; 2, pp. 31-46. [DOI: https://dx.doi.org/10.15212/CVIA.2016.0052] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/29034066]

193. Vallabhajosula, S.; Nikolopoulou, A.; Babich, J.W.; Osborne, J.R.; Tagawa, S.T.; Lipai, I.; Solnes, L.; Maresca, K.P.; Armor, T.; Joyal, J.L. et al. ^99mTc-Labeled Small-Molecule Inhibitors of Prostate-Specific Membrane Antigen: Pharmacokinetics and Biodistribution Studies in Healthy Subjects and Patients with Metastatic Prostate Cancer. J. Nucl. Med.; 2014; 55, pp. 1791-1798. [DOI: https://dx.doi.org/10.2967/jnumed.114.140426]

194. Goffin, K.E.; Joniau, S.; Tenke, P.; Slawin, K.; Klein, E.A.; Stambler, N.; Strack, T.; Babich, J.; Armor, T.; Wong, V. Phase 2 Study of ^99mTc-Trofolastat SPECT/CT to Identify and Localize Prostate Cancer in Intermediate- and High-Risk Patients Undergoing Radical Prostatectomy and Extended Pelvic LN Dissection. J. Nucl. Med.; 2017; 58, pp. 1408-1413. [DOI: https://dx.doi.org/10.2967/jnumed.116.187807]

195. Robu, S.; Schottelius, M.; Eiber, M.; Maurer, T.; Gschwend, J.; Schwaiger, M.; Wester, H.-J. Preclinical Evaluation and First Patient Application of ^99mTc-PSMA-I&S for SPECT Imaging and Radioguided Surgery in Prostate Cancer. J. Nucl. Med.; 2017; 58, pp. 235-242. [DOI: https://dx.doi.org/10.2967/jnumed.116.178939]

196. Evaluation of Tc 99m Tilmanocept Localization in Primary Cutaneous Kaposi’s Sarcoma and Lymphatic Drainage by SPECT/CT. Available online: https://clinicaltrials.gov/search?term=NCT02201420 (accessed on 15 August 2023).

197. Safety and Efficacy of Folatescan (Technetium TC 99M EC20) in Patients with Suspected Ovarian Carcinoma or Recurrent Endometrial Carcinoma. Available online: https://clinicaltrials.gov/search?term=NCT01689714%20 (accessed on 15 August 2023).

198. Technical Performance Assessment of Tc-99m Tetrofosmin for Differentiation of Recurrence vs. Radiation Necrosis in Patients with Glioma. Available online: https://clinicaltrials.gov/search?term=NCT02971319 (accessed on 15 August 2023).

199. Comparative Performance of Molecular Breast Imaging (MBI) to Magnetic Resonance Imaging (MRI) of the Breast in Identifying and Excluding Breast Carcinoma in Women at High Risk for Breast Cancer. Available online: https://clinicaltrials.gov/search?term=NCT05042687 (accessed on 15 August 2023).

200. Validation of 99mTc- EDDA–HYNIC -TOC Kits for Diagnosis of Neuroendocrine Tumors. Available online: https://clinicaltrials.gov/search?term=NCT02691078%20 (accessed on 15 August 2023).

201. Study to Evaluate 99mTc-MIP-1404 SPECT/CT Imaging in Men with Biopsy Proven Low-Grade Prostate Cancer. Available online: https://clinicaltrials.gov/search?term=NCT02615067%20 (accessed on 15 August 2023).

202. A Study of 99mTc-MIP-1404 and 99mTc-MIP-1405 in Patients with Metastatic Prostate Adenocarcinoma and Healthy Volunteers. Available online: https://clinicaltrials.gov/search?term=NCT01261754%20 (accessed on 15 August 2023).

203. Imaging Guided Surgery to Improve the Detection of Lymph Node Metastases in Prostate Cancer Patients. Available online: https://clinicaltrials.gov/search?term=NCT04832958%20 (accessed on 15 August 2023).

204. Predictive Value of 99mTc- Albumin Spheres Before 90Y- SIR Therapy. Available online: https://clinicaltrials.gov/search?term=NCT01186263 (accessed on 15 August 2023).

205. Jang, J.; Yamamoto, M.; Uesaka, M. Design of an X -band electron linear accelerator dedicated to decentralized ⁹⁹Mo/^99mTc supply: From beam energy selection to yield estimation. Phys. Rev. Accel. Beams; 2017; 20, 104701. [DOI: https://dx.doi.org/10.1103/PhysRevAccelBeams.20.104701]

206. Rovais, M.R.; Aardaneh, K.; Aslani, G.; Rahiminejad, A.; Yousefi, K.; Boulouri, F. Assessment of the direct cyclotron production of ^99mTc: An approach to crisis management of ^99mTc shortage. Appl. Radiat. Isot.; 2016; 112, pp. 55-61. [DOI: https://dx.doi.org/10.1016/j.apradiso.2016.03.017]

207. Dewulf, J.; Adhikari, K.; Vangestel, C.; Wyngaert, T.V.D.; Elvas, F. Development of Antibody Immuno-PET/SPECT Radiopharmaceuticals for Imaging of Oncological Disorders—An Update. Cancers; 2020; 12, 1868. [DOI: https://dx.doi.org/10.3390/cancers12071868] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/32664521]

208. Lubberink, M.; Tolmachev, V.; Widström, C.; Bruskin, A.; Lundqvist, H.; Westlin, J.E. 110mIn-DTPA-D-Phe1-octreotide for imaging of neuroendocrine tumors with PET. J. Nucl. Med.; 2002; 43, pp. 1391-1397. [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/12368379]

209. Salmanoglu, E.; Kim, S.; Thakur, M.L. Currently Available Radiopharmaceuticals for Imaging Infection and the Holy Grail. Semin. Nucl. Med.; 2018; 48, pp. 86-99. [DOI: https://dx.doi.org/10.1053/j.semnuclmed.2017.10.003]

210. Gawne, P.J.; Man, F.; Blower, P.J.; de Rosales, T.M.R. Direct Cell Radiolabeling for in Vivo Cell Tracking with PET and SPECT Imaging. Chem. Rev.; 2022; 122, pp. 10266-10318. [DOI: https://dx.doi.org/10.1021/acs.chemrev.1c00767]

211. Lewis, S.S.; Cox, G.M.; Stout, J.E. Clinical utility of indium 111-labeled white blood cell scintigraphy for evaluation of suspected infection. Open Forum Infect. Dis.; 2014; 1, ofu089. [DOI: https://dx.doi.org/10.1093/ofid/ofu089]

212. Han, M.; Partin, A.W. Current Clinical Applications of the In-capromab Pendetide Scan (ProstaScint(R) Scan, Cyt-356). Rev. Urol.; 2001; 3, pp. 165-171.

213. Morris, M.J.; Divgi, C.R.; Pandit-Taskar, N.; Batraki, M.; Warren, N.; Nacca, A.; Smith-Jones, P.; Schwartz, L.; Kelly, W.K.; Slovin, S. et al. Pilot trial of unlabeled and indium-111-labeled anti-prostate-specific membrane antigen antibody J591 for castrate metastatic prostate cancer. Clin. Cancer Res.; 2005; 11, pp. 7454-7461. [DOI: https://dx.doi.org/10.1158/1078-0432.CCR-05-0826] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/16243819]

214. Vallabhajosula, S.; Kuji, I.; Hamacher, K.A.; Konishi, S.; Kostakoglu, L.; Kothari, P.A.; Milowski, M.I.; Nanus, D.M.; Bander, N.H.; Goldsmith, S.J. Pharmacokinetics and biodistribution of 111In- and 177Lu-labeled J591 antibody specific for prostate-specific membrane antigen: Prediction of 90Y-J591 radiation dosimetry based on 111In or 177Lu?. J. Nucl. Med.; 2005; 46, pp. 634-641.

215. Hekman, M.C.; Rijpkema, M.; Muselaers, C.H.; Oosterwijk, E.; Hulsbergen-Van de Kaa, C.A.; Boerman, O.C.; Oyen, W.J.; Langenhuijsen, J.F.; Mulders, P.F. Tumor-targeted Dual-modality Imaging to Improve Intraoperative Visualization of Clear Cell Renal Cell Carcinoma: A First in Man Study. Theranostics; 2018; 8, pp. 2161-2170. [DOI: https://dx.doi.org/10.7150/thno.23335]

216. Gao, J.; Liao, Z.; Liu, W.; Hu, Y.; Ma, H.; Xia, L.; Li, F.; Lan, T.; Yang, Y.; Yang, J. et al. Simple and efficient method for producing high radionuclidic purity ¹¹¹In using enriched ¹¹²Cd target. Appl. Radiat. Isot.; 2021; 176, 109828. [DOI: https://dx.doi.org/10.1016/j.apradiso.2021.109828] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/34166947]

217. Radiolabelled CCK-2/Gastrin Receptor Analogue for Personalized Theranostic Strategy in Advanced MTC. Available online: https://clinicaltrials.gov/search?term=NCT03246659 (accessed on 15 August 2023).

218. 111In-ch806 in Patients with Advanced Tumours Expressing the 806 Antigen. Available online: https://clinicaltrials.gov/search?term=NCT00291447 (accessed on 15 August 2023).

219. A Phase I Pilot Study Comparing 123I MIP 1072 Versus 111In Capromab Pendetide in Subjects with Metastatic Prostate Cancer. Available online: https://clinicaltrials.gov/search?term=NCT00992745 (accessed on 15 August 2023).

220. Radio Guided Lymph Node Dissection in Oligometastatic Prost ate Cancer Patients. Available online: https://clinicaltrials.gov/search?term=NCT04300673 (accessed on 15 August 2023).

221. Intraoperative Dual-modality Imaging in Renal Cell Carcinoma. Available online: https://clinicaltrials.gov/search?term=NCT02497599 (accessed on 15 August 2023).

222. Trafficking of Indium-III-Labeled Cultured Immune Cells in Patients Undergoing Immunotherapy for Advanced Cancer. Available online: https://clinicaltrials.gov/search?term=NCT00026897 (accessed on 15 August 2023).

223. Jødal, L. Beta emitters and radiation protection. Acta Oncol.; 2009; 48, pp. 308-313. [DOI: https://dx.doi.org/10.1080/02841860802245163] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/18766999]

224. Sugyo, A.; Tsuji, A.B.; Sudo, H.; Okada, M.; Koizumi, M.; Satoh, H.; Kurosawa, G.; Kurosawa, Y.; Saga, T. Evaluation of Efficacy of Radioimmunotherapy with ⁹⁰Y-Labeled Fully Human Anti-Transferrin Receptor Monoclonal Antibody in Pancreatic Cancer Mouse Models. PLoS ONE; 2015; 10, e0123761. [DOI: https://dx.doi.org/10.1371/journal.pone.0123761]

225. Kletting, P.; Kull, T.; Maaß, C.; Malik, N.; Luster, M.; Beer, A.J.; Glatting, G. Optimized Peptide Amount and Activity for ⁹⁰Y-Labeled DOTATATE Therapy. J. Nucl. Med.; 2016; 57, pp. 503-508. [DOI: https://dx.doi.org/10.2967/jnumed.115.164699] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/26678617]

226. Tong, A.K.; Kao, Y.H.; Too, C.W.; Chin, K.F.; Ng, D.C.; Chow, P.K. Yttrium-90 hepatic radioembolization: Clinical review and current techniques in interventional radiology and personalized dosimetry. Br. J. Radiol.; 2016; 89, 20150943. [DOI: https://dx.doi.org/10.1259/bjr.20150943]

227. Ahmadzadehfar, H.; Biersack, H.J.; Ezziddin, S. Radioembolization of liver tumors with yttrium-90 microspheres. Semin. Nucl. Med.; 2010; 40, pp. 105-121. [DOI: https://dx.doi.org/10.1053/j.semnuclmed.2009.11.001]

228. Aranda, E.; Aparicio, J.; Bilbao, J.I.; García-Alfonso, P.; Maurel, J.; Rodríguez, J.; Sangro, B.; Vieitez, J.M.; Feliu, J. Recommendations for SIR-Spheres Y-90 resin microspheres in chemotherapy-refractory/intolerant colorectal liver metastases. Future Oncol.; 2017; 13, pp. 2065-2082. [DOI: https://dx.doi.org/10.2217/fon-2017-0220] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/28703622]

229. Sposito, C.; Mazzaferro, V. The SIRveNIB and SARAH trials, radioembolization vs. sorafenib in advanced HCC patients: Reasons for a failure, and perspectives for the future. Hepatobiliary Surg. Nutr.; 2018; 7, pp. 487-489. [DOI: https://dx.doi.org/10.21037/hbsn.2018.10.06]

230. Vilgrain, V.; Pereira, H.; Assenat, E.; Guiu, B.; Ilonca, A.D.; Pageaux, G.-P.; Sibert, A.; Bouattour, M.; Lebtahi, R.; Allaham, W. et al. Efficacy and safety of selective internal radiotherapy with yttrium-90 resin microspheres compared with sorafenib in locally advanced and inoperable hepatocellular carcinoma (SARAH): An open-label randomised controlled phase 3 trial. Lancet Oncol.; 2017; 18, pp. 1624-1636. [DOI: https://dx.doi.org/10.1016/S1470-2045(17)30683-6]

231. Alsultan, A.A.; Braat, A.; Smits, M.L.J.; Barentsz, M.W.; Bastiaannet, R.; Bruijnen, R.C.G.; de Keizer, B.; de Jong, H.; Lam, M.; Maccauro, M. et al. Current Status and Future Direction of Hepatic Radioembolisation. Clin. Oncol.; 2021; 33, pp. 106-116. [DOI: https://dx.doi.org/10.1016/j.clon.2020.12.003]

232. Garin, E.; Tselikas, L.; Guiu, B.; Chalaye, J.; Edeline, J.; de Baere, T.; Assenat, E.; Tacher, V.; Robert, C.; Terroir-Cassou-Mounat, M. et al. Personalised versus standard dosimetry approach of selective internal radiation therapy in patients with locally advanced hepatocellular carcinoma (DOSISPHERE-01): A randomised, multicentre, open-label phase 2 trial. Lancet Gastroenterol. Hepatol.; 2021; 6, pp. 17-29. [DOI: https://dx.doi.org/10.1016/S2468-1253(20)30290-9]

233. Wiseman, G.A.; Witzig, T.E. Yttrium-90 (⁹⁰Y) ibritumomab tiuxetan (Zevalin) induces long-term durable responses in patients with relapsed or refractory B-Cell non-Hodgkin’s lymphoma. Cancer Biother. Radiopharm.; 2005; 20, pp. 185-188. [DOI: https://dx.doi.org/10.1089/cbr.2005.20.185] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/15869453]

234. Sachpekidis, C.; Jackson, D.B.; Soldatos, T.G. Radioimmunotherapy in Non-Hodgkin’s Lymphoma: Retrospective Adverse Event Profiling of Zevalin and Bexxar. Pharmaceuticals; 2019; 12, 141. [DOI: https://dx.doi.org/10.3390/ph12040141]

235. Salles, G.; Barrett, M.; Foà, R.; Maurer, J.; O’Brien, S.; Valente, N.; Wenger, M.; Maloney, D.G. Rituximab in B-Cell Hematologic Malignancies: A Review of 20 Years of Clinical Experience. Adv. Ther.; 2017; 34, pp. 2232-2273. [DOI: https://dx.doi.org/10.1007/s12325-017-0612-x] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/28983798]

236. Juweid, M.E. Radioimmunotherapy of B-Cell Non-Hodgkin’s Lymphoma: From Clinical Trials to Clinical Practice. J. Nucl. Med.; 2002; 43, pp. 1507-1529. [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/12411555]

237. Houghton, J.L.; Membreno, R.; Abdel-Atti, D.; Cunanan, K.M.; Carlin, S.; Scholz, W.W.; Zanzonico, P.B.; Lewis, J.S.; Zeglis, B.M. Establishment of the In Vivo Efficacy of Pretargeted Radioimmunotherapy Utilizing Inverse Electron Demand Diels-Alder Click Chemistry. Mol. Cancer Ther.; 2017; 16, pp. 124-133. [DOI: https://dx.doi.org/10.1158/1535-7163.MCT-16-0503]

238. Paganelli, G.; Grana, C.; Chinol, M.; Cremonesi, M.; De Cicco, C.; De Braud, F.; Robertson, C.; Zurrida, S.; Casadio, C.; Zoboli, S. et al. Antibody-guided three-step therapy for high grade glioma with yttrium-90 biotin. Eur. J. Nucl. Med.; 1999; 26, pp. 348-357. [DOI: https://dx.doi.org/10.1007/s002590050397]

239. Knox, S.J.; Goris, M.L.; Tempero, M.; Weiden, P.L.; Gentner, L.; Breitz, H.; Adams, G.P.; Axworthy, D.; Gaffigan, S.; Bryan, K. et al. Phase II trial of yttrium-90-DOTA-biotin pretargeted by NR-LU-10 antibody/streptavidin in patients with metastatic colon cancer. Clin. Cancer Res.; 2000; 6, pp. 406-414.

240. Jallinoja, V.I.J.; Houghton, J.L. Current Landscape in Clinical Pretargeted Radioimmunoimaging and Therapy. J. Nucl. Med.; 2021; 62, pp. 1200-1206. [DOI: https://dx.doi.org/10.2967/jnumed.120.260687]

241. Paganelli, G.; De Cicco, C.; Ferrari, M.E.; McVie, G.; Pagani, G.; Leonardi, M.C.; Cremonesi, M.; Ferrari, A.; Pacifici, M.; Di Dia, A. et al. IART (Intra-Operative Avidination for Radionuclide Therapy) for accelerated radiotherapy in breast cancer patients. Technical aspects and preliminary results of a phase II study with 90Y-labelled biotin. Ecancermedicalscience; 2010; 4, 166. [DOI: https://dx.doi.org/10.3332/ecancer.2010.166]

242. Chinol, M.; Hnatowich, D.J. Generator-produced yttrium-90 for radioimmunotherapy. J. Nucl. Med.; 1987; 28, pp. 1465-1470.

243. Treatment of Patients with Advanced Renal Cancer with a Radiolabeled Antibody, Yttrium-90 Conjugated Chimeric G250. Available online: https://clinicaltrials.gov/search?term=NCT00199875 (accessed on 15 August 2023).

244. Yttrium Y 90 DOTA Anti-CEA Monoclonal Antibody M5A in Treating Patients with Advanced Solid Tumors. Available online: https://clinicaltrials.gov/search?term=NCT00645060 (accessed on 15 August 2023).

245. Radiolabeled Monoclonal Antibody Therapy, Combination Chemotherapy, and Bevacizumab in Treating Patients with Metastatic Colorectal Cancer. Available online: https://clinicaltrials.gov/search?term=NCT01205022 (accessed on 15 August 2023).

246. Safety and Efficacy Study of Different Doses of 90Y-hPAM4 Combined with Gemcitabine in Pancreatic Cancer. Available online: https://clinicaltrials.gov/search?term=NCT00603863 (accessed on 15 August 2023).

247. Neoadjuvant PRRT With Y-90-DOTATOC in pNET. Available online: https://clinicaltrials.gov/search?term=NCT05568017 (accessed on 15 August 2023).

248. Yttrium Y 90 SMT 487 in Treating Patients with Refractory or Recurrent Cancer. Available online: https://clinicaltrials.gov/search?term=NCT00006368 (accessed on 15 August 2023).

249. SorAfenib Versus Radioembolization in Advanced Hepatocellular Carcinoma. Available online: https://clinicaltrials.gov/search?term=NCT01482442%20 (accessed on 15 August 2023).

250. Y90 Ibritumomab Tiuxetan Post R-CHOP Chemotherapy for Advanced Stage Follicular Lymphoma. Available online: https://clinicaltrials.gov/search?term=NCT01446562 (accessed on 15 August 2023).

251. Uccelli, L.; Martini, P.; Urso, L.; Ghirardi, T.; Marvelli, L.; Cittanti, C.; Carnevale, A.; Giganti, M.; Bartolomei, M.; Boschi, A. Rhenium Radioisotopes for Medicine, a Focus on Production and Applications. Molecules; 2022; 27, 5283. [DOI: https://dx.doi.org/10.3390/molecules27165283] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/36014521]

252. Lepareur, N.; Lacœuille, F.; Bouvry, C.; Hindré, F.; Garcion, E.; Chérel, M.; Noiret, N.; Garin, E.; Knapp, F.F.R. Rhenium-188 Labeled Radiopharmaceuticals: Current Clinical Applications in Oncology and Promising Perspectives. Front. Med.; 2019; 6, 132. [DOI: https://dx.doi.org/10.3389/fmed.2019.00132]

253. Maxon, H.R., 3rd; Schroder, L.E.; Washburn, L.C.; Thomas, S.R.; Samaratunga, R.C.; Biniakiewicz, D.; Moulton, J.S.; Cummings, D.; Ehrhardt, G.J.; Morris, V. Rhenium-188(Sn)HEDP for treatment of osseous metastases. J. Nucl. Med.; 1998; 39, pp. 659-663.

254. De Decker, M.; Bacher, K.; Thierens, H.; Slegers, G.; Dierckx, R.A.; De Vos, F. In vitro and in vivo evaluation of direct rhenium-188-labeled anti-CD52 monoclonal antibody alemtuzumab for radioimmunotherapy of B-cell chronic lymphocytic leukemia. Nucl. Med. Biol.; 2008; 35, pp. 599-604. [DOI: https://dx.doi.org/10.1016/j.nucmedbio.2008.03.001]

255. Torres-García, E.; Ferro-Flores, G.; Arteaga de Murphy, C.; Correa-González, L.; Pichardo-Romero, P.A. Biokinetics and dosimetry of 188Re-anti-CD20 in patients with non-Hodgkin’s lymphoma: Preliminary experience. Arch. Med. Res.; 2008; 39, pp. 100-109. [DOI: https://dx.doi.org/10.1016/j.arcmed.2007.06.023]

256. Juweid, M.; Sharkey, R.M.; Swayne, L.C.; Griffiths, G.L.; Dunn, R.; Goldenberg, D.M. Pharmacokinetics, dosimetry and toxicity of rhenium-188-labeled anti-carcinoembryonic antigen monoclonal antibody, MN-14, in gastrointestinal cancer. J. Nucl. Med.; 1998; 39, pp. 34-42. [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/9443735]

257. Börjesson, P.K.; Postema, E.J.; Roos, J.C.; Colnot, D.R.; Marres, H.A.; van Schie, M.H.; Stehle, G.; de Bree, R.; Snow, G.B.; Oyen, W.J. et al. Phase I therapy study with ¹⁸⁶Re-labeled humanized monoclonal antibody BIWA 4 (bivatuzumab) in patients with head and neck squamous cell carcinoma. Clin. Cancer Res.; 2003; 9, pp. 3961s-3972s.

258. Rhenium-Skin Cancer Therapy (SCT) for the Treatment of Non-Melanoma Skin Cancer. Available online: https://clinicaltrials.gov/search?term=NCT05135052 (accessed on 15 August 2023).

259. Castellucci, P.; Savoia, F.; Farina, A.; Lima, G.M.; Patrizi, A.; Baraldi, C.; Zagni, F.; Vichi, S.; Pettinato, C.; Morganti, A.G. et al. High dose brachytherapy with non sealed ¹⁸⁸Re (rhenium) resin in patients with non-melanoma skin cancers (NMSCs): Single center preliminary results. Eur. J. Nucl. Med. Mol. Imaging; 2021; 48, pp. 1511-1521. [DOI: https://dx.doi.org/10.1007/s00259-020-05088-z] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/33140131]

260. Argyrou, M.; Valassi, A.; Andreou, M.; Lyra, M. Rhenium-188 production in hospitals, by w-188/re-188 generator, for easy use in radionuclide therapy. Int. J. Mol. Imaging; 2013; 2013, 290750. [DOI: https://dx.doi.org/10.1155/2013/290750] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/23653859]

261. Ehrhardt, G.J.; Ketring, A.R.; Ayers, L.M. Reactor-produced radionuclides at the University of Missouri Research Reactor. Appl. Radiat. Isot.; 1998; 49, pp. 295-297. [DOI: https://dx.doi.org/10.1016/S0969-8043(97)00038-9]

262. Rhenium-188-HEDP vs. Radium-223-chloride in Patients with Advanced Prostate Cancer Refractory to Hormonal Therapy. Available online: https://clinicaltrials.gov/search?term=NCT03458559 (accessed on 15 August 2023).

263. Biodistribution Study With 186 Re-labelled Humanised Monoclonal Antibody BIWA 4 in Patients with Non-small Cell Lung Cancer. Available online: https://clinicaltrials.gov/search?term=NCT02204059%20 (accessed on 15 August 2023).

264. Biodistribution Study With 186 Re-Labelled Humanised Monoclonal Antibody BIWA 4 in Patients with Adenocarcinoma of the Breast. Available online: https://clinicaltrials.gov/search?term=NCT02204046 (accessed on 15 August 2023).

265. Dose Escalation Study with 99mTC—Or 186 Re-labelled Humanised Monoclonal Antibody (hMAb) BIWA 4 in Patients with Head and Neck Cancer. Available online: https://clinicaltrials.gov/search?term=NCT02204033 (accessed on 15 August 2023).

266. Maximum Tolerated Dose, Safety, and Efficacy of Rhenium Nanoliposomes in Recurrent Glioma (ReSPECT). Available online: https://clinicaltrials.gov/search?term=NCT01906385 (accessed on 15 August 2023).

267. Vosoughi, S.; Shirvani-Arani, S.; Bahrami-Samani, A.; Salek, N.; Jalilian, A.R. Production of no-carrier-added Ho-166 for targeted therapy purposes. Iran. J. Nucl. Med.; 2017; 25, pp. 15-20.

268. Stella, M.; Braat, A.; van Rooij, R.; de Jong, H.; Lam, M. Holmium-166 Radioembolization: Current Status and Future Prospective. Cardiovasc. Intervent. Radiol.; 2022; 45, pp. 1634-1645. [DOI: https://dx.doi.org/10.1007/s00270-022-03187-y] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/35729423]

269. Mumper, R.J.; Ryo, U.Y.; Jay, M. Neutron-activated holmium-166-poly (L-lactic acid) microspheres: A potential agent for the internal radiation therapy of hepatic tumors. J. Nucl. Med.; 1991; 32, pp. 2139-2143.

270. Klaassen, N.J.M.; Arntz, M.J.; Gil Arranja, A.; Roosen, J.; Nijsen, J.F.W. The various therapeutic applications of the medical isotope holmium-166: A narrative review. EJNMMI Radiopharm. Chem.; 2019; 4, 19. [DOI: https://dx.doi.org/10.1186/s41181-019-0066-3]

271. Dash, A.; Pillai, M.R.; Knapp, F.F., Jr. Production of ¹⁷⁷Lu for Targeted Radionuclide Therapy: Available Options. Nucl. Med. Mol. Imaging; 2015; 49, pp. 85-107. [DOI: https://dx.doi.org/10.1007/s13139-014-0315-z]

272. Kim, K.; Kim, S.J. Lu-177-Based Peptide Receptor Radionuclide Therapy for Advanced Neuroendocrine Tumors. Nucl. Med. Mol. Imaging; 2018; 52, pp. 208-215. [DOI: https://dx.doi.org/10.1007/s13139-017-0505-6]

273. Yadav, M.P.; Ballal, S.; Sahoo, R.K.; Dwivedi, S.N.; Bal, C. Radioligand Therapy With 177Lu-PSMA for Metastatic Castration-Resistant Prostate Cancer: A Systematic Review and Meta-Analysis. Am. J. Roentgenol.; 2019; 213, pp. 275-285. [DOI: https://dx.doi.org/10.2214/AJR.18.20845] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/30995089]

274. Weineisen, M.; Schottelius, M.; Simecek, J.; Baum, R.P.; Yildiz, A.; Beykan, S.; Kulkarni, H.R.; Lassmann, M.; Klette, I.; Eiber, M. et al. 68Ga- and 177Lu-Labeled PSMA I&T: Optimization of a PSMA-Targeted Theranostic Concept and First Proof-of-Concept Human Studies. J. Nucl. Med.; 2015; 56, pp. 1169-1176. [DOI: https://dx.doi.org/10.2967/jnumed.115.158550] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/26089548]

275. Strosberg, J.; El-Haddad, G.; Wolin, E.; Hendifar, A.; Yao, J.; Chasen, B.; Mittra, E.; Kunz, P.L.; Kulke, M.H.; Jacene, H. et al. Phase 3 Trial of ¹⁷⁷Lu-Dotatate for Midgut Neuroendocrine Tumors. N. Engl. J. Med.; 2017; 376, pp. 125-135. [DOI: https://dx.doi.org/10.1056/NEJMoa1607427]

276. Strosberg, J.R.; Caplin, M.E.; Kunz, P.L.; Ruszniewski, P.B.; Bodei, L.; Hendifar, A.E.; Mittra, E.; Wolin, E.M.; Yao, J.C.; Pavel, M.E. et al. Final overall survival in the phase 3 NETTER-1 study of lutetium-177-DOTATATE in patients with midgut neuroendocrine tumors. J. Clin. Oncol.; 2021; 39, 4112. [DOI: https://dx.doi.org/10.1200/JCO.2021.39.15_suppl.4112]

277. Cankaya, A.; Balzer, M.; Amthauer, H.; Brenner, W.; Spreckelmeyer, S. Optimization of ¹⁷⁷Lu-labelling of DOTA-TOC, PSMA-I&T and FAPI-46 for clinical application. EJNMMI Radiopharm. Chem.; 2023; 8, 10. [DOI: https://dx.doi.org/10.1186/s41181-023-00196-1]

278. Ballal, S.; Yadav, M.; Moon, E.S.; Kumari, S.; Roesch, F.; Tripathi, M.; Tupalli, A.; Bal, C. First clinical experience and initial outcomes of ¹⁷⁷Lu-DOTAGA.(SA.FAPi₂ therapy in patients with end-stage radioiodine-refractory differentiated thyroid cancer: A Salvage treatment option. J. Nucl. Med.; 2021; 62, 1701.

279. Study of 177Lu-PSMA-617 In Metastatic Castrate-Resistant Prostate Cancer (VISION). Available online: https://classic.clinicaltrials.gov/ct2/show/NCT03511664 (accessed on 17 August 2023).

280. Combination of Radium-223 and Lutetium-177 PSMA-I&T in Men with Metastatic Castration-Resistant Prostate Cancer (AlphaBet). Available online: https://clinicaltrials.gov/study/NCT05383079?term=Lu177&page=4&rank=37 (accessed on 17 August 2023).

281. Study of Cabozantinib with Lu-177 in Patients with Somatostatin Receptor 2 Positive Neuroendocrine Tumors. Available online: https://clinicaltrials.gov/study/NCT05249114?term=Lu177&rank=3 (accessed on 17 August 2023).

282. Capecitabine ON Temozolomide Radionuclide Therapy Octreotate Lutetium-177 NeuroEndocrine Tumours Study (CONTROL NETS). Available online: https://clinicaltrials.gov/study/NCT02358356?term=Lu177&page=5&rank=42 (accessed on 17 August 2023).

283. Lu-177-DOTATATE (Lutathera) in Therapy of Inoperable Pheochromocytoma/Paraganglioma. Available online: https://clinicaltrials.gov/study/NCT03206060?term=Lu177&page=2&rank=12 (accessed on 17 September 2023).

284. Lutetium 177Lu-Edotreotide Versus Best Standard of Care in Well-Differentiated Aggressive Grade-2 and Grade-3 GastroEnteroPancreatic NeuroEndocrine Tumors (GEP-NETs)—COMPOSE (COMPOSE). Available online: https://classic.clinicaltrials.gov/ct2/show/NCT04919226 (accessed on 17 September 2023).

285. Clinical Application of Lutetium [177Lu]-Catalase in Tumor Radionuclide Therapy. Available online: https://clinicaltrials.gov/study/NCT05985278?term=Lu177&rank=5 (accessed on 17 September 2023).

286. Diagnosis of Metastatic Tumors on 68Ga-FAPI PET-CT and Radioligand Therapy With 177Lu-EB-FAPI. Available online: https://clinicaltrials.gov/study/NCT05400967?term=Lu177&rank=6 (accessed on 17 September 2023).

287. Phase II Study of Lutetium-177 Labeled Girentuximab in Patients with Advanced Renal Cancer. Available online: https://clinicaltrials.gov/study/NCT02002312?term=Lu177&rank=8 (accessed on 17 September 2023).

288. Chakravarty, R.; Chakraborty, S. A review of advances in the last decade on targeted cancer therapy using ¹⁷⁷Lu: Focusing on ¹⁷⁷Lu produced by the direct neutron activation route. Am. J. Nucl. Med. Mol. Imaging; 2021; 11, pp. 443-475.

289. Eychenne, R.; Chérel, M.; Haddad, F.; Guérard, F.; Gestin, J.F. Overview of the Most Promising Radionuclides for Targeted Alpha Therapy: The “Hopeful Eight”. Pharmaceutics; 2021; 13, 906. [DOI: https://dx.doi.org/10.3390/pharmaceutics13060906] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/34207408]

290. Jurcic, J.G.; Larson, S.M.; Sgouros, G.; McDevitt, M.R.; Finn, R.D.; Divgi, C.R.; Ballangrud, A.M.; Hamacher, K.A.; Ma, D.; Humm, J.L. et al. Targeted alpha particle immunotherapy for myeloid leukemia. Blood; 2002; 100, pp. 1233-1239. [DOI: https://dx.doi.org/10.1182/blood.V100.4.1233.h81602001233_1233_1239]

291. Rosenblat, T.L.; McDevitt, M.R.; Mulford, D.A.; Pandit-Taskar, N.; Divgi, C.R.; Panageas, K.S.; Heaney, M.L.; Chanel, S.; Morgenstern, A.; Sgouros, G. et al. Sequential cytarabine and alpha-particle immunotherapy with bismuth-213-lintuzumab (HuM195) for acute myeloid leukemia. Clin. Cancer Res.; 2010; 16, pp. 5303-5311. [DOI: https://dx.doi.org/10.1158/1078-0432.CCR-10-0382]

292. Bruchertseifer, F.; Kellerbauer, A.; Malmbeck, R.; Morgenstern, A. Targeted alpha therapy with bismuth-213 and actinium-225: Meeting future demand. J. Labelled Comp. Radiopharm.; 2019; 62, pp. 794-802. [DOI: https://dx.doi.org/10.1002/jlcr.3792] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/31369165]

293. Ahenkorah, S.; Cassells, I.; Deroose, C.M.; Cardinaels, T.; Burgoyne, A.R.; Bormans, G.; Ooms, M.; Cleeren, F. Bismuth-213 for Targeted Radionuclide Therapy: From Atom to Bedside. Pharmaceutics; 2021; 13, 599. [DOI: https://dx.doi.org/10.3390/pharmaceutics13050599]

294. Kratochwil, C.; Giesel, F.L.; Bruchertseifer, F.; Mier, W.; Apostolidis, C.; Boll, R.; Murphy, K.; Haberkorn, U.; Morgenstern, A. ²¹³Bi-DOTATOC receptor-targeted alpha-radionuclide therapy induces remission in neuroendocrine tumours refractory to beta radiation: A first-in-human experience. Eur. J. Nucl. Med. Mol. Imaging; 2014; 41, pp. 2106-2119. [DOI: https://dx.doi.org/10.1007/s00259-014-2857-9]

295. Sathekge, M.; Knoesen, O.; Meckel, M.; Modiselle, M.; Vorster, M.; Marx, S. ²¹³Bi-PSMA-617 targeted alpha-radionuclide therapy in metastatic castration-resistant prostate cancer. Eur. J. Nucl. Med. Mol. Imaging; 2017; 44, pp. 1099-1100. [DOI: https://dx.doi.org/10.1007/s00259-017-3657-9]

296. Kvassheim, M.; Revheim, M.-E.R.; Stokke, C. Quantitative SPECT/CT imaging of lead-212: A phantom study. EJNMMI Phys.; 2022; 9, 52. [DOI: https://dx.doi.org/10.1186/s40658-022-00481-z]

297. Robertson, A.K.H.; Ramogida, C.F.; Rodríguez-Rodríguez, C.; Blinder, S.; Kunz, P.; Sossi, V.; Schaffer, P. Multi-isotope SPECT imaging of the ²²⁵Ac decay chain: Feasibility studies. Phys. Med. Biol.; 2017; 62, pp. 4406-4420. [DOI: https://dx.doi.org/10.1088/1361-6560/aa6a99] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/28362640]

298. Morgenstern, A.; Bruchertseifer, F.; Apostolidis, C. Bismuth-213 and actinium-225—Generator performance and evolving therapeutic applications of two generator-derived alpha-emitting radioisotopes. Curr. Radiopharm.; 2012; 5, pp. 221-227. [DOI: https://dx.doi.org/10.2174/1874471011205030221] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/22642390]

299. Chemotherapy and Monoclonal Antibody Therapy in Treating Patients with Advanced Myeloid Cancer. Available online: https://clinicaltrials.gov/search?term=NCT00014495 (accessed on 15 August 2023).

300. Nelson, B.J.B.; Andersson, J.D.; Wuest, F. Targeted Alpha Therapy: Progress in Radionuclide Production, Radiochemistry, and Applications. Pharmaceutics; 2020; 13, 49. [DOI: https://dx.doi.org/10.3390/pharmaceutics13010049] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/33396374]

301. Jurcic, J.G.; Rosenblat, T.L.; McDevitt, M.R.; Pandit-Taskar, N.; Carrasquillo, J.A.; Chanel, S.M.; Ryan, C.; Frattini, M.G.; Cicic, D.; Larson, S.M. et al. Phase I trial of the targeted alpha-particle nano-generator actinium-225 (225Ac-lintuzumab) (anti-CD33; HuM195) in acute myeloid leukemia (AML). J. Clin. Oncol.; 2011; 29, 6516. [DOI: https://dx.doi.org/10.1200/jco.2011.29.15_suppl.6516]

302. Juergens, R.A.; Zukotynski, K.A.; Juneau, D.; Krnezich, L.; Simms, R.; Forbes, J.; Burak, E.S.; Valliant, J.; Stafford, L.; Armor, T. et al. A phase I study of [225Ac]-FPI-1434 radioimmunotherapy in patients with IGF-1R expressing solid tumors. J. Clin. Oncol.; 2019; 37, TPS3152. [DOI: https://dx.doi.org/10.1200/JCO.2019.37.15_suppl.TPS3152]

303. Kratochwil, C.; Bruchertseifer, F.; Rathke, H.; Bronzel, M.; Apostolidis, C.; Weichert, W.; Haberkorn, U.; Giesel, F.L.; Morgenstern, A. Targeted α-Therapy of Metastatic Castration-Resistant Prostate Cancer with ²²⁵Ac-PSMA-617: Dosimetry Estimate and Empiric Dose Finding. J. Nucl. Med.; 2017; 58, pp. 1624-1631. [DOI: https://dx.doi.org/10.2967/jnumed.117.191395]

304. Venetoclax and Lintuzumab-Ac225 in AML Patients. Available online: https://clinicaltrials.gov/search?term=NCT03867682%20 (accessed on 15 August 2023).

305. Radioimmunotherapy (111Indium/225Actinium-DOTA-daratumumab) for the Treatment of Relapsed/Refractory Multiple Myeloma. Available online: https://clinicaltrials.gov/search?term=NCT05363111%20 (accessed on 15 August 2023).

306. A Phase 1/2 Study of [225Ac]-FPI-1434 Injection. Available online: https://clinicaltrials.gov/search?term=NCT03746431%20 (accessed on 15 August 2023).

307. Actinium 225 Labeled Anti-CEA Antibody (Ac225-DOTA-M5A) for the Treatment of CEA Producing Advanced or Metastatic Cancers. Available online: https://clinicaltrials.gov/search?term=NCT05204147%20 (accessed on 15 August 2023).

308. Study of 225Ac-PSMA-617 in Men with PSMA-positive Prostate Cancer. Available online: https://clinicaltrials.gov/search?term=NCT04597411 (accessed on 15 August 2023).

309. Phase I Trial of 225Ac-J591 in Patients With mCRPC. Available online: https://clinicaltrials.gov/search?term=NCT03276572%20 (accessed on 15 August 2023).

310. Weidner, J.W.; Mashnik, S.G.; John, K.D.; Ballard, B.; Birnbaum, E.R.; Bitteker, L.J.; Couture, A.; Fassbender, M.E.; Goff, G.S.; Gritzo, R. et al. ²²⁵Ac and ²²³Ra production via 800 MeV proton irradiation of natural thorium targets. Appl. Radiat. Isot.; 2012; 70, pp. 2590-2595. [DOI: https://dx.doi.org/10.1016/j.apradiso.2012.07.003] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/22944532]

311. Li, M.; Zhang, X.; Quinn, T.P.; Lee, D.; Liu, D.; Kunkel, F.; Zimmerman, B.E.; McAlister, D.; Olewein, K.; Menda, Y. et al. Automated cassette-based production of high specific activity [²⁰³/²¹²Pb]peptide-based theranostic radiopharmaceuticals for image-guided radionuclide therapy for cancer. Appl. Radiat. Isot.; 2017; 127, pp. 52-60. [DOI: https://dx.doi.org/10.1016/j.apradiso.2017.05.006]

312. McNeil, B.L.; Robertson, A.K.H.; Fu, W.; Yang, H.; Hoehr, C.; Ramogida, C.F.; Schaffer, P. Production, purification, and radiolabeling of the 203Pb/212Pb theranostic pair. EJNMMI Radiopharm. Chem.; 2021; 6, 6. [DOI: https://dx.doi.org/10.1186/s41181-021-00121-4]

313. Meredith, R.F.; Torgue, J.; Azure, M.T.; Shen, S.; Saddekni, S.; Banaga, E.; Carlise, R.; Bunch, P.; Yoder, D.; Alvarez, R. Pharmacokinetics and imaging of 212Pb-TCMC-trastuzumab after intraperitoneal administration in ovarian cancer patients. Cancer Biother. Radiopharm.; 2014; 29, pp. 12-17. [DOI: https://dx.doi.org/10.1089/cbr.2013.1531]

314. Meredith, R.; Torgue, J.; Shen, S.; Fisher, D.R.; Banaga, E.; Bunch, P.; Morgan, D.; Fan, J.; Straughn, J.M., Jr. Dose escalation and dosimetry of first-in-human α radioimmunotherapy with ²¹²Pb-TCMC-trastuzumab. J. Nucl. Med.; 2014; 55, pp. 1636-1642. [DOI: https://dx.doi.org/10.2967/jnumed.114.143842] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/25157044]

315. Meredith, R.F.; Torgue, J.J.; Rozgaja, T.A.; Banaga, E.P.; Bunch, P.W.; Alvarez, R.D.; Straughn, J.M., Jr.; Dobelbower, M.C.; Lowy, A.M. Safety and Outcome Measures of First-in-Human Intraperitoneal α Radioimmunotherapy With ²¹²Pb-TCMC-Trastuzumab. Am. J. Clin. Oncol.; 2018; 41, pp. 716-721. [DOI: https://dx.doi.org/10.1097/COC.0000000000000353] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/27906723]

316. Delpassand, E.S.; Tworowska, I.; Esfandiari, R.; Torgue, J.; Hurt, J.; Shafie, A.; Núñez, R. Targeted α-Emitter Therapy with ²¹²Pb-DOTAMTATE for the Treatment of Metastatic SSTR-Expressing Neuroendocrine Tumors: First-in-Humans Dose-Escalation Clinical Trial. J. Nucl. Med.; 2022; 63, pp. 1326-1333. [DOI: https://dx.doi.org/10.2967/jnumed.121.263230]

317. Targeted Alpha-emitter Therapy of PRRT Naive Neuroendocrine Tumor Patients. Available online: https://clinicaltrials.gov/search?term=NCT05153772 (accessed on 15 August 2023).

318. Safety Study of ²¹²Pb-TCMC-Trastuzumab Radio Immunotherapy. Available online: https://clinicaltrials.gov/search?term=NCT01384253 (accessed on 15 August 2023).

319. Kokov, K.V.; Egorova, B.V.; German, M.N.; Klabukov, I.D.; Krasheninnikov, M.E.; Larkin-Kondrov, A.A.; Makoveeva, K.A.; Ovchinnikov, M.V.; Sidorova, M.V.; Chuvilin, D.Y. ²¹²Pb: Production Approaches and Targeted Therapy Applications. Pharmaceutics; 2022; 14, 189. [DOI: https://dx.doi.org/10.3390/pharmaceutics14010189]

320. Sartor, O.; Sharma, D. Radium and other alpha emitters in prostate cancer. Transl. Androl. Urol.; 2018; 7, pp. 436-444. [DOI: https://dx.doi.org/10.21037/tau.2018.02.07]

321. Flux, G.D. Imaging and dosimetry for radium-223: The potential for personalized treatment. Br. J. Radiol.; 2017; 90, 20160748. [DOI: https://dx.doi.org/10.1259/bjr.20160748]

322. Akabani, G.; Kennel, S.J.; Zalutsky, M.R. Microdosimetric Analysis of α-Particle-Emitting Targeted Radiotherapeutics Using Histological Images. J. Nucl. Med.; 2003; 44, pp. 792-805. [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/12732682]

323. Sgouros, G.; Hobbs, R.F. Dosimetry for radiopharmaceutical therapy. Semin. Nucl. Med.; 2014; 44, pp. 172-178. [DOI: https://dx.doi.org/10.1053/j.semnuclmed.2014.03.007] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/24832581]

324. Pacilio, M.; Ventroni, G.; De Vincentis, G.; Cassano, B.; Pellegrini, R.; Di Castro, E.; Frantellizzi, V.; Follacchio, G.A.; Garkavaya, T.; Lorenzon, L. et al. Dosimetry of bone metastases in targeted radionuclide therapy with alpha-emitting ²²³Ra-dichloride. Eur. J. Nucl. Med. Mol. Imaging; 2016; 43, pp. 21-33. [DOI: https://dx.doi.org/10.1007/s00259-015-3150-2]

325. Gott, M.; Steinbach, J.; Mamat, C. The Radiochemical and Radiopharmaceutical Applications of Radium. Open Chem.; 2016; 14, pp. 118-129. [DOI: https://dx.doi.org/10.1515/chem-2016-0011]

326. Jadvar, H.; Quinn, D.I. Targeted α-particle therapy of bone metastases in prostate cancer. Clin. Nucl. Med.; 2013; 38, pp. 966-971. [DOI: https://dx.doi.org/10.1097/RLU.0000000000000290] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/24212441]

327. Ji, Y.Y.; Chung, K.H.; Lim, J.M.; Kim, C.J.; Jang, M.; Kang, M.J.; Park, S.T.; Woo, Z.; Koo, B.; Seo, B. Feasibility about the direct measurement of 226 Ra using the gamma-ray spectrometry. J. Nucl. Fuel Cycle Waste Technol.; 2014; 12, pp. 97-105. [DOI: https://dx.doi.org/10.7733/jnfcwt.2014.12.2.97]

328. Westrøm, S.; Generalov, R.; Bønsdorff, T.B.; Larsen, R.H. Preparation of 212Pb-labeled monoclonal antibody using a novel ²²⁴Ra-based generator solution. Nucl. Med. Biol.; 2017; 51, pp. 1-9. [DOI: https://dx.doi.org/10.1016/j.nucmedbio.2017.04.005]

329. Lassmann, M.; Eberlein, U. Comparing absorbed doses and radiation risk of the α-emitting bone-seekers [²²³Ra]RaCl(2) and [²²⁴Ra]RaCl(2). Front. Med.; 2022; 9, 1057373. [DOI: https://dx.doi.org/10.3389/fmed.2022.1057373]

330. Juzeniene, A.; Bernoulli, J.; Suominen, M.; Halleen, J.; Larsen, R.H. Antitumor Activity of Novel Bone-seeking, α-emitting ²²⁴Ra-solution in a Breast Cancer Skeletal Metastases Model. Anticancer. Res.; 2018; 38, pp. 1947-1955. [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/29599310]

331. Wick, R.R.; Atkinson, M.J.; Nekolla, E.A. Incidence of leukaemia and other malignant diseases following injections of the short-lived α-emitter ²²⁴Ra into man. Radiat. Environ. Biophys.; 2009; 48, pp. 287-294. [DOI: https://dx.doi.org/10.1007/s00411-009-0227-y] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/19475414]

332. Arazi, L.; Cooks, T.; Schmidt, M.; Keisari, Y.; Kelson, I. Treatment of solid tumors by interstitial release of recoiling short-lived alpha emitters. Phys. Med. Biol.; 2007; 52, 5025. [DOI: https://dx.doi.org/10.1088/0031-9155/52/16/021] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/17671351]

333. Popovtzer, A.; Rosenfeld, E.; Mizrachi, A.; Bellia, S.R.; Ben-Hur, R.; Feliciani, G.; Sarnelli, A.; Arazi, L.; Deutsch, L.; Kelson, I. et al. Initial Safety and Tumor Control Results From a “First-in-Human” Multicenter Prospective Trial Evaluating a Novel Alpha-Emitting Radionuclide for the Treatment of Locally Advanced Recurrent Squamous Cell Carcinomas of the Skin and Head and Neck. Int. J. Radiat. Oncol. Biol. Phys.; 2020; 106, pp. 571-578. [DOI: https://dx.doi.org/10.1016/j.ijrobp.2019.10.048]

334. Nilsson, S.; Larsen, R.H.; Fosså, S.D.; Balteskard, L.; Borch, K.W.; Westlin, J.E.; Salberg, G.; Bruland, O.S. First clinical experience with alpha-emitting radium-223 in the treatment of skeletal metastases. Clin. Cancer Res.; 2005; 11, pp. 4451-4459. [DOI: https://dx.doi.org/10.1158/1078-0432.CCR-04-2244] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/15958630]

335. Florimonte, L.; Dellavedova, L.; Maffioli, L.S. Radium-223 dichloride in clinical practice: A review. Eur. J. Nucl. Med. Mol. Imaging; 2016; 43, pp. 1896-1909. [DOI: https://dx.doi.org/10.1007/s00259-016-3386-5]

336. Vuong, W.; Sartor, O.; Pal, S.K. Radium-223 in metastatic castration resistant prostate cancer. Asian J. Androl.; 2014; 16, pp. 348-353. [DOI: https://dx.doi.org/10.4103/1008-682x.127812] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/24713838]

337. Phase I Dose Escalation of Monthly Intravenous Ra-223 Dichloride in Osteosarcoma. Available online: https://clinicaltrials.gov/search?term=NCT01833520 (accessed on 15 August 2023).

338. Phase IB Trial of Radium-223 and Niraparib in Patients with Castrate Resistant Prostate Cancer (NiraRad). Available online: https://clinicaltrials.gov/search?term=NCT03076203 (accessed on 15 August 2023).

339. Radium-223 Dichloride and Abiraterone Acetate Compared to Placebo and Abiraterone Acetate for Men with Cancer of the Prostate When Medical or Surgical Castration Does Not Work and When the Cancer Has Spread to the Bone, Has Not Been Treated with Chemotherapy and Is Causing No Or Only Mild Symptoms (ERA 223). Available online: https://clinicaltrials.gov/search?term=NCT02043678 (accessed on 15 August 2023).

340. Radium Ra 223 With Enzalutamide Compared to Enzalutamide Alone in Men with Metastatic Castration Refractory Prostate Cancer. Available online: https://clinicaltrials.gov/search?term=NCT02199197%20 (accessed on 15 August 2023).

341. The Combination Therapy with Ra-223 and Enzalutamide. Available online: https://clinicaltrials.gov/search?term=NCT03305224 (accessed on 15 August 2023).

342. Trial of Ra-223 Dichloride in Combination with Hormonal Therapy and Denosumab in the Treatment of Patients with Hormone-Positive Bone-Dominant Metastatic Breast Cancer. Available online: https://clinicaltrials.gov/search?term=NCT02366130%20 (accessed on 15 August 2023).

343. Phase Ib Study of Radium Ra 223 Dichloride in Combination with Paclitaxel in Cancer Subjects with Bone Lesions. Available online: https://clinicaltrials.gov/search?term=NCT02442063%20 (accessed on 15 August 2023).

344. A Study to Test Radium-223 With Docetaxel in Patients with Prostate Cancer. Available online: https://clinicaltrials.gov/search?term=NCT03574571 (accessed on 15 August 2023).

345. Radium Ra 223 Dichloride, Hormone Therapy and Stereotactic Body Radiation Therapy in Treating Patients with Metastatic Prostate Cancer. Available online: https://clinicaltrials.gov/search?term=NCT03361735 (accessed on 15 August 2023).

346. Study Evaluating the Addition of Pembrolizumab to Radium-223 in mCRPC. Available online: https://clinicaltrials.gov/search?term=NCT03093428 (accessed on 15 August 2023).

347. Safety and Tolerability of Atezolizumab (ATZ) in Combination with Radium-223 Dichloride (R-223-D) in Metastatic Castrate-Resistant Prostate Cancer (CRPC) Progressed Following Treatment with an Androgen Pathway Inhibitor. Available online: https://clinicaltrials.gov/search?term=NCT02814669 (accessed on 15 August 2023).

348. Alpha Radiation Emitters Device for the Treatment of Advanced Pancreatic Cancer. Available online: https://clinicaltrials.gov/search?term=NCT04002479%20 (accessed on 15 August 2023).

349. Alpha Radiation Emitters Device for the Treatment of Newly Diagnosed Breast Carcinoma Patients with Distant Metastases. Available online: https://clinicaltrials.gov/search?term=NCT03970967 (accessed on 15 August 2023).

350. Abou, D.S.; Pickett, J.; Mattson, J.E.; Thorek, D.L.J. A Radium-223 microgenerator from cyclotron-produced trace Actinium-227. Appl. Radiat. Isot.; 2017; 119, pp. 36-42. [DOI: https://dx.doi.org/10.1016/j.apradiso.2016.10.015] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/27835737]

351. Pruszyński, M.; Walczak, R.; Rodak, M.; Bruchertseifer, F.; Morgenstern, A.; Bilewicz, A. Radiochemical separation of 224Ra from 232U and 228Th sources for 224Ra/212Pb/212Bi generator. Appl. Radiat. Isot.; 2021; 172, 109655. [DOI: https://dx.doi.org/10.1016/j.apradiso.2021.109655]

352. Murray, I.; Rojas, B.; Gear, J.; Callister, R.; Cleton, A.; Flux, G.D. Quantitative Dual-Isotope Planar Imaging of Thorium-227 and Radium-223 Using Defined Energy Windows. Cancer Biother. Radiopharm.; 2020; 35, pp. 530-539. [DOI: https://dx.doi.org/10.1089/cbr.2019.3554]

353. Karlsson, J.; Schatz, C.A.; Wengner, A.M.; Hammer, S.; Scholz, A.; Cuthbertson, A.; Wagner, V.; Hennekes, H.; Jardine, V.; Hagemann, U.B. Targeted thorium-227 conjugates as treatment options in oncology. Front. Med.; 2022; 9, 1071086. [DOI: https://dx.doi.org/10.3389/fmed.2022.1071086] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/36726355]

354. Heyerdahl, H.; Abbas, N.; Sponheim, K.; Mollatt, C.; Bruland, Ø.; Dahle, J. Targeted Alpha Therapy with 227Th-trastuzumab of Intraperitoneal Ovarian Cancer in Nude Mice. Curr. Radiopharm.; 2013; 6, pp. 106-116. [DOI: https://dx.doi.org/10.2174/18744710113069990018]

355. Study to Evaluate the Safety, Tolerability, Pharmacokinetics, and Antitumor Activity of a Thorium-227 Labeled Antibody-Chelator Conjugate Alone and in Combination With Darolutamide, in Patients with Metastatic Castration Resistant Prostate Cancer. Available online: https://clinicaltrials.gov/search?term=NCT03724747 (accessed on 15 August 2023).

356. Hammer, S.; Zitzmann-Kolbe, S.; Oden, F.; Jannsen, J.; Bickel, E.; Mueller, A.; Everz, L.; Hagemann, U.B.; Karlsson, J.; Ryan, O.B. et al. Abstract 1141: Preclinical analysis of biodistribution and PET imaging of a zirconium-89 labeled PSMA-targeted antibody-chelator conjugate. Cancer Res.; 2019; 79, 1141. [DOI: https://dx.doi.org/10.1158/1538-7445.AM2019-1141]

357. Ermolaev, S.V.; Zhuikov, B.L.; Kokhanyuk, V.M.; Matushko, V.L.; Kalmykov, S.N.; Aliev, R.A.; Tananaev, I.G.; Myasoedov, B.F. Production of actinium, thorium and radium isotopes from natural thorium irradiated with protons up to 141 MeV. Radiochim. Acta; 2012; 100, pp. 223-229. [DOI: https://dx.doi.org/10.1524/ract.2012.1909]

358. First-in-human Study of BAY2287411 Injection, a Thorium-227 Labeled Antibody-chelator Conjugate, in Patients with Tumors Known to Express Mesothelin. Available online: https://clinicaltrials.gov/search?term=NCT03507452 (accessed on 15 August 2023).

359. A First in Human Study of BAY2701439 to Look at Safety, How the Body Absorbs, Distributes and Excretes the Drug, and How Well the Drug Works in Participants with Advanced Cancer Expressing the HER2 Protein. Available online: https://clinicaltrials.gov/search?term=NCT04147819 (accessed on 15 August 2023).

360. Safety and Tolerability of BAY1862864 Injection in Subjects with Relapsed or Refractory CD22-Positive Non-Hodgkin’s Lymphoma. Available online: https://clinicaltrials.gov/search?term=NCT02581878%20 (accessed on 15 August 2023).

361. Lindegren, S.; Albertsson, P.; Bäck, T.; Jensen, H.; Palm, S.; Aneheim, E. Realizing Clinical Trials with Astatine-211: The Chemistry Infrastructure. Cancer Biother. Radiopharm.; 2020; 35, pp. 425-436. [DOI: https://dx.doi.org/10.1089/cbr.2019.3055]

362. Zalutsky, M.R.; Reardon, D.A.; Akabani, G.; Coleman, R.E.; Friedman, A.H.; Friedman, H.S.; McLendon, R.E.; Wong, T.Z.; Bigner, D.D. Clinical experience with alpha-particle emitting 211At: Treatment of recurrent brain tumor patients with 211At-labeled chimeric antitenascin monoclonal antibody 81C6. J. Nucl. Med.; 2008; 49, pp. 30-38. [DOI: https://dx.doi.org/10.2967/jnumed.107.046938] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/18077533]

363. Hallqvist, A.; Bergmark, K.; Bäck, T.; Andersson, H.; Dahm-Kähler, P.; Johansson, M.; Lindegren, S.; Jensen, H.; Jacobsson, L.; Hultborn, R. et al. Intraperitoneal α-Emitting Radioimmunotherapy with ²¹¹At in Relapsed Ovarian Cancer: Long-Term Follow-up with Individual Absorbed Dose Estimations. J. Nucl. Med.; 2019; 60, pp. 1073-1079. [DOI: https://dx.doi.org/10.2967/jnumed.118.220384] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/30683761]

364. Guérard, F.; Gestin, J.F.; Brechbiel, M.W. Production of [²¹¹At]-astatinated radiopharmaceuticals and applications in targeted α-particle therapy. Cancer Biother. Radiopharm.; 2013; 28, pp. 1-20. [DOI: https://dx.doi.org/10.1089/cbr.2012.1292] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/23075373]

365. Larsen, R.H.; Wieland, B.W.; Zalutsky, M.R. Evaluation of an internal cyclotron target for the production of 211At via the 209Bi (α,2n)211At reaction. Appl. Radiat. Isot.; 1996; 47, pp. 135-143. [DOI: https://dx.doi.org/10.1016/0969-8043(95)00285-5] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/8852627]

366. Crawford, J.R.; Yang, H.; Kunz, P.; Wilbur, D.S.; Schaffer, P.; Ruth, T.J. Development of a preclinical ²¹¹Rn/²¹¹At generator system for targeted alpha therapy research with ²¹¹At. Nucl. Med. Biol.; 2017; 48, pp. 31-35. [DOI: https://dx.doi.org/10.1016/j.nucmedbio.2017.01.011] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/28193502]

367. Targeted Alpha Therapy Using Astatine (At-211) Against Differentiated Thyroid Cancer. Available online: https://clinicaltrials.gov/search?term=NCT05275946 (accessed on 15 August 2023).

368. Radiolabeled Monoclonal Antibody Therapy in Treating Patients with Primary or Metastatic Brain Tumors. Available online: https://clinicaltrials.gov/search?term=NCT00003461%20 (accessed on 15 August 2023).

369. Total Body Irradiation and Astatine-211-Labeled BC8-B10 Monoclonal Antibody for the Treatment of Nonmalignant Diseases. Available online: https://clinicaltrials.gov/search?term=NCT04083183 (accessed on 15 August 2023).

370. 211At-BC8-B10 Followed by Donor Stem Cell Transplant in Treating Patients with Relapsed or Refractory High-Risk Acute Leukemia or Myelodysplastic Syndrome. Available online: https://clinicaltrials.gov/search?term=NCT03670966 (accessed on 15 August 2023).

371. Radioimmunotherapy (211At-OKT10-B10) and Chemotherapy (Melphalan) Before Stem Cell Transplantation for the Treatment of Multiple Myeloma. Available online: https://clinicaltrials.gov/search?term=NCT04466475 (accessed on 15 August 2023).