Abstract

Background: The microbial communities residing in the mosquito midgut play a key role in determining the outcome of mosquito pathogen infection. Elizabethkingia anophelis, originally isolated from the midgut of Anopheles gambiae, has drawn much attention due to its close association with Aedes and Anopheles mosquitoes, primary vectors of dengue virus and malaria parasites, respectively. E. anophelis possesses a broad-spectrum antiviral phenotype, yet a gap in knowledge regarding the mechanistic basis of its interaction with viruses exists. Methodology/Principal findings: To further understand the antiviral interactions between E. anophelis and Zika virus (ZIKV), we utilized a non-targeted multi-omics approach, analyzing lipids, proteins, and metabolites of cell monolayers co-infected with ZIKV and E. anophelis. We further assessed the gene expression of ZIKV when cultured in the presence of E. anophelis. ZIKV cultured in the presence of E. anophelis resulted in an attenuated replicative fitness and unproductive virus infection. Further, in this treatment, we observed lower levels of the nonstructural protein 5 (NS5) and RNA-directed RNA polymerase (RdRp) protein. Lastly, a significant decrease in arginine levels, an essential requirement for viral replication and progression of viral infection was observed. Conclusions/Significance: This study provides insights into the molecular basis of E. anophelis antiviral phenotype. These findings improve our knowledge of how microbes and viruses interact to impact viral replication. In the future, our findings can be utilized to unravel the mechanism behind the antiviral phenotype of E. anophelis, and this can help develop novel paradigms for viral therapeutics.

Competing Interest Statement

The authors have declared no competing interest.

Details

Title
Multi-Omics Analysis Of Antiviral Interactions Of Elizabethkingia anophelis And Zika Virus
Author
Sultana Fatema Omme; Wang, Junyao; Sifuna, Millicent; Rodriguez, Jacqueline; Owusu, Rhoda; Goli, Mona; Jiang, Peilin; Wazia Purba; Nwaiwu, Judith; Brelsfoard, Corey; Vigneron, Aurelien; Kramer, Laura; Ciota, Alexander; Mechref, Yehia; Onyango, Maria Gorreti
University/institution
Cold Spring Harbor Laboratory Press
Section
New Results
Publication year
2023
Publication date
Dec 27, 2023
Publisher
Cold Spring Harbor Laboratory Press
ISSN
2692-8205
Source type
Working Paper
Language of publication
English
ProQuest document ID
2906615768
Copyright
© 2023. This article is published under http://creativecommons.org/licenses/by/4.0/ (“the License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.