Abstract

The consumption of bovine milk and its derivatives is associated with inflammation, gastrointestinal disorders and the development of diseases in humans. Most studies related to milk effects are based on either clinal trials or experimental models such as mice and cell cultures. In this study we present the nematode Caenorhabditis elegans as an alternative model to evaluate the effects of milk on oxidative stress in other animal models. The toxicological effect of 20% milk exposure for 8 h on C. elegans was evaluated by progeny quantification, body size and pharyngeal pumping rate. Treating the worms with milk did not affect the worms brood size but interfered with their fecundity by delaying the average number of eggs in the first day of oviposition when compared to the control group. The size of worms treated with milk were significantly smaller compared to control. The pharyngeal pumping rate of milk-treated animals was not significantly different compared to untreated animals. Taking together, the results suggest that 20% milk treatment is not toxic for the worms but induces a minor delay achieving its adulthood and therefore its reproduction period. Milk exposure did not reduce the worms’ survival under stress conditions and increase endogenous ROS levels. This study contributes to characterize the effects of milk exposure on the C. elegans nematode.

Details

Title
Effect of milk exposure on the redox profile of Caenorhabditis elegans
Author
de Oliveira, Ingrid Laíse Silvestre 1   VIAFID ORCID Logo  ; Silva, Giovanna Melo Martins 2   VIAFID ORCID Logo  ; Cadavid, Cesar Orlando Muñoz 2   VIAFID ORCID Logo  ; Sales, Danielle Cavalcanti 1   VIAFID ORCID Logo  ; Anaya, Katya 3   VIAFID ORCID Logo  ; de Paula Oliveira, Riva 4   VIAFID ORCID Logo  ; do Nascimento Rangel, Adriano Henrique 1   VIAFID ORCID Logo 

 Universidade Federal do Rio Grande do Norte, Escola Agrícola de Jundiaí, Unidade Acadêmica Especializada em Ciências Agrárias, Macaíba, Brazil (GRID:grid.411233.6) (ISNI:0000 0000 9687 399X) 
 Universidade Federal do Rio Grande do Norte, Rede Nordeste de Biotecnologia (RENORBIO), Natal, Brazil (GRID:grid.411233.6) (ISNI:0000 0000 9687 399X) 
 Universidade Federal do Rio Grande do Norte, Faculdade de Ciências da Saúde Do Trairí (FACISA), Santa Cruz, , Brazil (GRID:grid.411233.6) (ISNI:0000 0000 9687 399X) 
 Universidade Federal do Rio Grande do Norte, Departamento de Biologia Celular E Genética, Natal, Brazil (GRID:grid.411233.6) (ISNI:0000 0000 9687 399X) 
Pages
1926
Publication year
2024
Publication date
2024
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2917422351
Copyright
© The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.