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Abstract

A first generation clone-based physical map for the bovine genome was constructed combining, fluorescent double digestion fingerprinting and sequence tagged site (STS) marker screening. The BAC clones were selected from an Inra BAC library (105 984 clones) and a part of the CHORI-240 BAC library (26 500 clones). The contigs were anchored using the screening information for a total of 1303 markers (451 microsatellites, 471 genes, 127 EST, and 254 BAC ends). The final map, which consists of 6615 contigs assembled from 100 923 clones, will be a valuable tool for genomic research in ruminants, including targeted marker production, positional cloning or targeted sequencing of regions of specific interest.

Details

Title
A first generation bovine BAC-based physical map
Author
Schibler, Laurent 1 ; Roig, Anne 1 ; Mahé, Marie-Françoise 1 ; Save, Jean-Claude 2 ; Gautier, Mathieu 1 ; Taourit, Sead 1 ; Boichard, Didier 3 ; Eggen, André 1 ; Cribiu, Edmond P. 1 

1  Institut national de la recherche agronomique, Centre de recherche de Jouy, Laboratoire de génétique biochimique et de cytogénétique, Département de génétique animale, Jouy-en-Josas Cedex, France 
2  Institut national de la recherche agronomique, Centre de recherche de Jouy, Laboratoire de radiobiologie et d'étude du génome, Département de génétique animale, Jouy-en-Josas Cedex, France 
3  Institut national de la recherche agronomique, Centre de recherche de Jouy, Station de génétique quantitative appliquée, Département de génétique animale, Jouy-en-Josas Cedex, France 
Pages
105
Publication year
2004
Publication date
Feb 2004
Publisher
Springer Nature B.V.
ISSN
0999193X
e-ISSN
12979686
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/1297-9686-36-1-105
ProQuest document ID
2922308760
Copyright
© INRA, EDP Sciences 2004.