Abstract

Given the growing interest in the role of zinc in the onset and progression of diseases, there is a crucial demand for reliable methods to modulate zinc homeostasis. Using a dietary approach, we provide validated strategies to alter whole-body zinc in mice, applicable across species. For confirmation of zinc status, animal growth rates as well as plasma and urine zinc levels were evaluated. The accessible and cost-effective methodology outlined will increase scientific rigor, ensuring reproducibility in studies exploring the impact of zinc deficiency and repletion on the onset and progression of diseases.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

* Slightly revised text to increase clarity of methods

Details

Title
Strategies for Inducing and Validating Zinc Deficiency and Zinc Repletion
Author
Tara-Yesomi Wenegieme; Elased, Dalia; Rockwood, Jananie; Khanzada Hasrat; Mcmichael, Kelia; Marshall, Andrea; Neikirk, Kit; Kirabo, Annet; Elased, Khalid; Hinton, Antentor, Jr; Williams, Clintoria
University/institution
Cold Spring Harbor Laboratory Press
Section
New Results
Publication year
2024
Publication date
Mar 10, 2024
Publisher
Cold Spring Harbor Laboratory Press
ISSN
2692-8205
Source type
Working Paper
Language of publication
English
ProQuest document ID
2937170042
Copyright
© 2024. This article is published under http://creativecommons.org/licenses/by-nd/4.0/ (“the License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.