Abstract
In this randomized, crossover, pilot clinical study, we aimed to evaluate the effect of supplementation of enzymes-probiotics blend with whey protein on the amino acid absorption and gut microbiota. Healthy subjects were supplemented with the whey protein and test i.e. Pepzyme Pro (enzymes-probiotics blend) or placebo i.e. maltodextrin for 15 days with the washout period of 30 days. Blood samples were analyzed for plasma free amino acids, insulin, and CRP. Additionally, urine nitrogen, fecal nitrogen, and gut microbiota were evaluated. On day 15, the test arm showed upward trend in rate of amino acid absorption than placebo arm within 30 min of post ingestion of protein. Moreover, rate of absorption of few essential and branched chain amino acids were significantly higher (methionine (p = 0.049), leucine (p = 0.014), isoleucine (p = 0.053)) in the test arm on day 15. Total branched chain amino acids absorption were found to be significantly higher (p ≤ 0.05) in the test arm than the placebo arm within 30 min of post ingestion on day 15. Uptrend in total amino acid absorption and Cmax, and downtrend in Tmax was observed on day 15 in the test arm. The CRP, fecal nitrogen, and urine nitrogen remained unaltered after supplementation. Microbiota profiling showed significant change in abundance of species of genus Bacteroides and phylum Bacteroidetes. Overall, metagenomics and metabolomics based assessments demonstrated that the consumption of Pepzyme Pro with whey protein could potentially improve protein digestion, amino acid absorption, and modulate gut microbiota.
Clinical trial registration The clinical trial registry of India CTRI/2021/09/036169 [Registered on: 02/09/2021]
Article Highlights
Uptrend in the rate of amino acid absorption and total amino acid absorption after consumption of whey protein with enzymes-probiotics blend for 15 days.
Significantly higher rate of absorption of branched chain amino acids after consumption of whey protein with enzymes-probiotics blend for 15 days.
Positive alteration in gut microbiota after consumption of whey protein with enzymes-probiotics blend for 15 days.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer
Details
1 Advanced Enzymes Technologies Ltd., Human nutrition department, Louiswadi, Thane, India
2 Leucine Rich Bio Pvt. Ltd., Bangalore, India