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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Ovine herpesvirus 2 (OvHV-2) and bovine herpesvirus 4 (BoHV-4) are gamma herpesviruses that belong to the genera Macavirus and Rhadinovirus, respectively. As with all herpesviruses, both OvHV-2 and BoHV-4 express glycoprotein B (gB), which plays an essential role in the infection of host cells. In that context, it has been demonstrated that a BoHV-4 gB-null mutant is unable to infect host cells. In this study, we used homologous recombination to insert OvHV-2 ORF 8, encoding gB, into the BoHV-4 gB-null mutant genome, creating a chimeric BoHV-4 virus carrying and expressing OvHV-2 gB (BoHV-4∆gB/OvHV-2-gB) that was infectious and able to replicate in vitro. We then evaluated BoHV-4∆gB/OvHV-2-gB as a potential vaccine candidate for sheep-associated malignant catarrhal fever (SA-MCF), a fatal disease of ungulates caused by OvHV-2. Using rabbits as a laboratory model for MCF, we assessed the safety, immunogenicity, and efficacy of BoHV-4∆gB/OvHV-2-gB in an immunization/challenge trial. The results showed that while BoHV-4∆gB/OvHV-2-gB was safe and induced OvHV-2 gB-specific humoral immune responses, immunization conferred only 28.5% protection upon challenge with OvHV-2. Therefore, future studies should focus on alternative strategies to express OvHV-2 proteins to develop an effective vaccine against SA-MCF.

Details

Title
Ovine Herpesvirus 2 Glycoprotein B Complementation Restores Infectivity to a Bovine Herpesvirus 4 gB-Null Mutant
Author
Moré, Daniela D 1   VIAFID ORCID Logo  ; Baker, Katherine N 1 ; Shringi, Smriti 2   VIAFID ORCID Logo  ; Bastos, Reginaldo G 3 ; Donal O’Toole 4   VIAFID ORCID Logo  ; Donofrio, Gaetano 5 ; Cunha, Cristina W 3   VIAFID ORCID Logo 

 Animal Disease Research Unit, Agricultural Research Service, United States Department of Agriculture, Pullman, WA 99164, USA; [email protected] (K.N.B.); [email protected] (R.G.B.); [email protected] (C.W.C.) 
 Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA; [email protected] 
 Animal Disease Research Unit, Agricultural Research Service, United States Department of Agriculture, Pullman, WA 99164, USA; [email protected] (K.N.B.); [email protected] (R.G.B.); [email protected] (C.W.C.); Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA; [email protected] 
 Department of Animal Science, University of Wyoming, Laramie, WY 82070, USA; [email protected] 
 Department of Medical-Veterinary Science, University of Parma, 43126 Parma, Italy; [email protected] 
First page
219
Publication year
2024
Publication date
2024
Publisher
MDPI AG
e-ISSN
20760817
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3003348387
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.