Abstract: The tea plant is a valuable and evergreen crop that is extensively cultivated in China and many other countries. Currently, there is growing research interest in this plant. For the tea industry, it is crucial to develop rapid and non-invasive methods to evaluate tea plants in their natural environment. This article provides a comprehensive overview of non-invasive sensing techniques used for in-situ detection of tea plants. The topics covered include leaf, canopy, and field-level assessments, as well as statistical analysis techniques and characteristics specific to the research. Non-invasive testing technology is primarily used for monitoring and predicting tea pests and diseases, monitoring quality, and nutrients, determining tenderness and grade, identifying tea plant varieties, automatically detecting, and identifying tea buds, monitoring tea plant growth, and extracting tea garden areas through remote sensing. It also helps to evaluate planting suitability, assess disasters, and estimate yields. Additionally, the article examines the challenges and prospects of emerging techniques aimed at resolving the in-situ detection problem for tea plants. It can assist researchers and producers in comprehensively understanding the tea environment, quality characteristics, and growth process, thereby enhancing tea production quality, and fostering tea industry development.
Keywords: non-destructive, in-situ detection, tea plants, growth characteristics, sensors
1 Introduction
Tea is a beneficial beverage due to its compounds such as tea polyphenols, amino acids, and vitamins, which possess medicinal qualities[1]. Tea plants are extensively cultivated, and farms are distributed globally. The tea garden ecosystem is a controlled semiartificial ecosystem that is distinct from agricultural and woodland ecosystems. It has a great capacity for absorbing carbon and makes a significant contribution to reducing carbon emissions. Traditional detection methods for tea plants rely heavily on manual labor and are often subject to. For example, tea range and yield are currently monitored by collecting census data and conducting field surveys, while tea quality and growth estimation are mainly through manual identification or chemical analysis. These results are often inconsistent and less objective12 . In recent years, non-destructive detection techniques such as remote sensing image resources (RSI), near-infrared spectroscopy (NIRS), hyperspectral imaging (HSI), synthetic aperture radar (SAR), and digital cameras have enabled rapid detection of tea plants.
Remote sensing is a powerful tool for monitoring tea plants and their environment. It provides timely, cost-effective, and large-scale information on tea plants, without requiring direct contact. Multispectral satellite sources are often used to monitor the distribution of tea gardens™. The Vis/NIR spectra between 400 to 2500 nm can be used to analyze vegetation status by studying the absorption of the atomic groups™ and predicting related components. Numerous studies have focused on the quantitative analysis of chemicals present in tea, such as caffeine™, tea polyphenols™, and amino acids™ using different NIRS apparatus. Technologies that involve mechanics, optics, electromagnetic sensing, digital video, and image processing can provide objective and accurate information for assessing tea quality, and enhancing tea production efficiency™.
Recently, there has been extensive research on in-situ monitoring of tea plants using non-destructive techniques to evaluate their quality and nutritional value. Chen et al.™ provided a summary of the application of NIRS, EN, ET, and computer vision techniques for this purpose, while Zhu et al.™ focused on the use of NIRS alone. Lin et al.[10] reviewed various vibrational spectroscopic techniques, including NIR, MIR, Raman, terahertz (THz) spectroscopy, and HSI technologies, for tea quality and safety analysis. These methods, combined with machine learning and neural networks, have the potential to quantitatively predict tea quality components and evaluate tea safety. Despite several reviews, there has been no discussion on in-situ detection for tea plant monitoring. Based on existing research, this review aims to fill that gap by evaluating the use of these non-destructive techniques for in-situ monitoring of tea plants at various scales, as shown in Figure 1.
2 Leaf-scale monitoring for tea quality and stress
To ensure optimal tea quality and detect tea leaf stress, sensors are utilized to measure the physical and chemical properties of tea leaves, including nutrition, grades, and other parameters. These sensors can also identify the specific diseases and pests affecting the tea leaves. Figure 2 illustrates the significant role of leaf-scale monitoring in the tea plant.
2.1 Disease and pest identification of tea
Due to a lack of experienced tea plant protection experts and environmental data, tea growers may struggle to accurately diagnose diseases and pests. It is a significant issue for the tea industry, which requires precise identification to maintain yield and production quality. One solution to this problem is the use of computer vision systems and deep learning models[11]. For instance, Li et al.[13] proposed using a four-channeled residual network (F-
RNet) based on R-CNN and wavelet trans-form on RGB images to identify the diseases and pests of tea leaves. Karmokar et al.[14] developed a tea leaf disease identifier that integrates a neural network to recognize diseases by extracting features from tea leaf images. Additionally, HSI is effective for tea plant phenotyping and
insect infection identification. Researchers have used HSI to detect tea anthracnose[15] and other tea diseases and pests by combining wavelength analysis with HSI data[16]. There are also non-destructive techniques available for identifying tea diseases and pests which are listed in Table 1.
2.2 Tea quality and nutrition monitoring
Traditionally, the quality and nutritional value of fresh tea leaves have been assessed through sensory evaluation by experienced tasters, which is not always reliable. To address this issue, various nondestructive methods have been developed to control the quality of tea leaves. These methods detect tea polyphenols[25], caffeine, nitrogen, phosphorus, potassium, chlorophyll, and moisture. Furthermore, machine learning and deep learning techniques have been utilized to estimate tea quality content from HSI, multispectral,
and NIR information. The combination of nondestructive techniques with chemometrics or machine learning methods has been successful in determining tea leaf quality and nutrition. For instance, Wang et al.[26] utilized the hyperspectral technique in conjunction with SPA-MLR to estimate the moisture, total nitrogen, and crude fiber contents of fresh tea leaves. Sun et al.[27] used NIR-HSI to visualize the distribution of moisture content in tea leaves. Zhang et al.[28] predicted the moisture content of tea leaves through the NIRS technique. A detailed summary of the related research is listed in Table 2.
2.3 Tea tenderness and grades classification
The freshness of tea leaves is crucial in producing high-quality tea. Processing tea to a standard requires accurately determining the freshness of the leaves. A variety of factors can affect the tea quality, including their growing surroundings, position on the plant[41], and time of harvest[42]. Tea leaf images provide valuable biological information such as leaf shape, size, and edges, which can be used to differentiate tea grades, varieties, and tenderness. Tang et al.[43] proposed a method for sorting green tea leaves based on texture extraction using non-overlapping window local binary patterns (LBP) and gray level co-occurrence matrix (GLCM). This approach has been successfully employed in the automatic tea processing lines to capture tea leaves from conveyor belts. Additionally, Zhang et al.[37] introduced a non-invasive Raman spectroscopy scanning method to evaluate the tenderness of fresh tea leaves. Table 3 compiles various non-destructive approaches for a range of tea-related classifications, e.g., tea grade, leaf position, variety, geographical origin, and harvest season identification.
3 Canopy-scale growth monitoring by optical sensors
The quality of tea products is influenced by the cultivars, growing conditions, and processing techniques used. In tea gardens, the raw materials used in tea production are crucial in determining the highest potential quality of tea. Therefore, it is essential to monitor tea plant growth accurately and quickly on a field scale. Optical sensors are a valuable tool that can measure various aspects of the tea plant canopy, such as its structure, color, and shape, as well as the amount of light, water, and nutrients it receives, and the level of photosynthesis taking place. This information can be used to optimize tea plant growth by efficiently allocating resources and ensuring optimal conditions. Figure 3 shows the significance of canopy-scale monitoring of tea plants.
3.1 Tea cultivar classification
The yield, quality[50], resistance, and flavor of tea plants can differ significantly depending on the variety being grown. Moreover, different cultivars have different responses to vegetative and reproductive growth[51]. The appearance of different tea cultivars, including the color and texture of the shoots, leaves, plant shape, yield, and bloom of one bud with one or two leaves, is evaluated by experts. However, identifying different tea cultivars is a complex and challenging task that currently depends on DNA sequencing analysis[52] or empirical experts. Each tea cultivar is suited to different tea products. For instance, Fuding dabai is ideal for Baimudan, while Longjing 43 is excellent for Longjing tea processing. Over the last two decades, reflectance spectra classification has been used increasingly to determine tea plant species, as Nidamanuri[53], used hyperspectral data to differentiate nine tea plant varieties and six other natural plants in a tea garden. Cao et al.[54] deployed multispectral imaging technology to discern 16 high-yield tea plant cultivars, and Tu et al.[55] used UAV-HSI to classify tea cultivars based on canopy information. Table 4 lists insight into the research that scientists have conducted to classify tea cultivars and recognize tea plants using rapid and nondestructive methods.
3.2 Tea shoot detection and recognition
Mechanical and automatic tea picking is essential for tea plantations due to the high cost and labor-intensive nature of traditional manual tea harvesting. Tea shoot detection and recognition in real-time is necessary. However, detecting tea shoots can be challenging due to the complex lighting conditions and numerous canopy structures. The traditional image processing approach uses shape, color, and texture algorithms for tea shoot recognition. Using a fusion of traditional image processing algorithms along with deep learning techniques has enhanced the accuracy of tea shoot detection. Several approaches, such as edge detection[60], improved deep convolutional encoder-decoder network[61], faster R-CNN and FCN techniques[62], RGB-D camara[63], and two-level fusion network[64], have been proposed for tea shoot detection and recognition. YOLOv3 has become a widely adopted computer version system for tea shoot recognition^. Table 5 lists the commonly used techniques and algorithms for tea shoot detection.
3.3 Tea plant growth monitoring
Monitoring the growth of tea plants involves using digital and spectral information platforms to collect images and textures of the tea canopy. These images are then used to create quantitative models of tea quality, nutrients, and pigments using statistical analysis, machine learning, or deep learning algorithms. For instance, some studies have used multivariate analysis with hyperspectral data to predict polyphenols[47], nitrogen[72], chlorophyll[31], and plant behaviors under different management practices[73]. Other studies have utilized various imaging systems to evaluate tea quality components or diagnose tea plant stress. Kang et al.[74] applied the HSI to estimate the catechins content of fresh tea leaves in green tea fields and achieve 0.79 precision. Cui et al.[75] detested the tea plant stress using HSI. Chen et al.[76] deployed a multispectral imaging system to evaluate tea quality components with 0.85 accuracies at a canopy level. Wang et al.[77] developed a smartphone-based micro near-infrared spectrometer system to diagnose tea pigments. Cao et al.[78] combined multispectral images and hyperspectral data of tea plant canopy to predict nitrogen content. Luo et al.[79] employed UAV image data of the tea plant canopy to monitor polyphenols, nitrogen, and amino acid contents. Tu et al.[55] utilized a hyperspectral camera mounted on a UAV to monitor the tea polyphenols and amino acids of tea plants and achieved good accuracy. Real-time monitoring of tea growth through these technologies provides support for improving the information management level and intelligent operation of tea gardens. In the future, thermal infrared and mid-infrared imaging technology may also be used to monitor tea plants.
4 Field-scale monitoring for scientific tea garden management
The tea plantation industry plays a crucial role in the development of rural economies in China. To effectively monitor and evaluate tea gardens, field-scale monitoring of tea plants is essential. By swiftly extracting information from tea planting areas, this technique aids in monitoring tea growth, predicting disasters, and estimating tea yield. Furthermore, it assists in optimizing the use of tea garden resources and leveraging the exceptional ecological geological environment. Figure 4 displays the procedural steps for conducting field-scale monitoring of tea plants.
4.1 Tea plantations extraction and dynamic changes evaluation
Accurately obtaining the dynamic distribution and planting area of tea plants is crucial for the government to evaluate the tea industry. Due to the unique texture features of tea gardens, high-altitude remote sensing via satellite can effectively monitor them[82]. Several studies have utilized high spatiotemporal-resolution multispectral imagery to evaluate the spatial distribution and area of tea plantations, including Li et al.[83], Huang et al.[84], and Dihkan et al.[85] Various satellite resources, such as Sentinel-2[3] and WorldView-2[86], have been used to examine the distribution and extraction of tea plantations. In addition, remote sensing technologies such as Landsat[87], SAR[88], Lidar[89], and hyperspectral data[90] are also utilized for tea garden classification. When applying remote sensing technology to tea plantation extraction, color, texture, spectral, and terrain features are considered, including NDVI[86'91], MNDVI[85], EVI[88], MNDWI[88], LSWI[88], GLCM texture[86], Gabor texture[86], DEM[88]. Furthermore, machine learning or deep learning methods coupled with multiple remote sensing technologies have been used to differentiate tea cultivars from other vegetation. Table 6 provides a summary of various non-destructive remote sensing data sources used for tea plantation extraction.
4.2 Tea cultivation suitability assessment by remote sensing and spatial analysis
The production and quality of tea are heavily reliant on various environmental factors, including soil suitability, precipitation, temperature, and moisture[98]. Assessing the suitability of tea plant growth is crucial for establishing a successful tea plantation1". The geographical origins of tea produce varying flavors and tastes due to diverse factors such as climate, environment, altitude, soil, and topography. It is vital to identify the optimal climate and soil to guarantee the suitable growth and harvesting of tea. Previous studies have employed various methodologies, including the Delphi method and GIS[100], Sentinel-2 image data[81], and integrating Sentinel-2 images with GIS[101] to evaluate tea plantation suitability. Factors considered for tea cultivation suitability include climate[102], soil[103], terrain, human-related factors, ecological economy factors, and tea plant growth information[104]. Accurate regional demarcation and assessment of tea plantations can significantly enhance tea planting, boost tea quality, and provide a scientific basis for tea cultivation.
4.3 Frost damage warning of tea plants
Tea cultivation is often hampered by cold and frost which can significantly lower yields and deteriorate tea quality[105]. To prevent and manage such occurrences, it is critical to conduct a phenological analysis of tea gardens. This analysis can assist the agricultural department in improving preparedness. Kimura et al.[106] demonstrated a spatiotemporal distribution of the thermal effects of frost-protective fans in a tea field by modeling the cold tolerance of tea buds based on temperature patterns[107]. Additionally, they proposed a sensible assessment of tea frost risk, using 1km (2) -gridded meteorological data[108]. Kotikot et al.[92] utilized MODIS-derived LST to detect frost occurrences in tea plantations, and further employed MODIS and risk factors associated with topography and land surface temperature[109]. Wu et al.[110] put forth a real-time forecasting system for evaluating frost damage degrees by using a small weather station to validate parameters, resulting in accurate prediction of the frost damage. Wang et al.[111] monitored and assessed spring frost in tea plants in China through MODIS data and established a minimum temperature (Tmin) elevation model based on reconstructed MYD11A1 nighttime LST values for 3x3 pixel windows and digital elevation model data[112]. They integrated the satellite-based Tmin estimates and ground-based Tmin observations to analyze the spring frost damage of tea plants from 2003 to 2020 in China, which offers effective guidance for farmers. Xu et al.[80] developed a frost-hazard warning system by combining topography, meteorology, longitude, latitude, and machine learning models in the Zhejiang Province of China. It is essential to conduct monitoring research on tea plant frost to determine the occurrence and degree of damage. Analyzing the distribution of tea frost can serve as a reference for frost prevention and guidance for adjusting tea planting planning and layout.
4.4 Tea yield estimation
Accurately estimating agricultural yield is essential for decision-making regarding prices, exports, and imports, as well as increasing food security and ensuring a sufficient food supply[113]. Tea yield is influenced by various factors, including crown density, and can be determined by remote sensing imagery. Several studies have utilized different satellite information and models to estimate tea yield, including the use of SPOT-7 and ALOS AVNIR-2 images[114], multi-temporal MODIS NDVI data[115], RF model with SVR-based feature selection[116], 1RS-1C images[117], SAVI extracted from Landsat-8 OLI and Sentinel-2B images[118], and the FAO AquaCrop simulation model[119]. A summary of the data resources, models, analysis methods, and results of tea yield estimation is provided in Table 7.
5 Discussion
In the past decade, research into the monitoring of tea plants using non-destructive methods has increased, and considerable results have been obtained. In-situ detection has gained extensive research attention and has become a popular subject in the tea industry and market. Several commonly used non-destructive techniques share advantages including rapid, efficient, and convenient. However, each technique has its own specific advantages and disadvantages when applied to address various issues concerning tea plants.
5.1 Limitations of tea quality at leaf-scale
Monitoring of tea growth and stress of tea at a leaf scale is limited in its ability to detect stress on a large scale. To establish a reliable model for monitoring tea quality, a wide and representative range of leaf samples must be collected. The dynamic monitoring of tea quality is challenged by the fluctuation of water content in fresh leaves. The classification of tea tenderness and grade is also affected by overlapping leaves. Identifying the grade of stacked leaves and determining the proportion of different grades remains a difficult problem to solve.
5.2 Difficulties in tea growth monitoring at canopy-scale
Sensors are commonly utilized to capture the spectral images of the tea plant canopy. However, the accuracy of monitoring tea plant growth is contingent on various factors, including the density of tea germination, bud, and leaf tenderness, and the collection area. Non-destructive testing technology is essential due to the significant impact of water and fertilizer status, as well as the water content of tea leaves, on the quality components. While canopy-scale monitoring provides a comprehensive view of tea plant growth and quality, it may not immediately detect the effects of microenvironments on growth. Furthermore, outdoor light can cause errors in the model's accuracy by interfering with spectral information. The tea bud recognition environment is complex and can be affected by overlap, occlusion, light shadow, species, and tenderness, all of which can affect the outcome.
5.3 Uncertainty of tea garden area extraction and yield estimation by remote sensing images
Utilizing remote sensing images is a valuable approach for identifying tea planting areas on a large scale and obtaining information on the spatial distribution and planting area of tea plants in a dynamic and continuous manner. However, conducting field investigations can be challenging due to the extensive distribution of tea gardens and mountainous planting areas. The accuracy of identifying tea gardens with images can also be affected by various factors such as terrain, weather, and other vegetation in the area, resulting in limited extraction of single source data. Various studies employ distinct remote sensing data sources and classification techniques to extract tea plantations in different regions. Despite exhibiting favorable recognition precision, the outcomes of these studies cannot be compared, making it challenging to assess the impact of integrating diverse data sources and classification methods. Therefore, multi-source remote sensing data and time series data, integrated intelligent algorithm has become a growing trend for extracting tea plant information at a large scale. The yield of tea is influenced by various factors including picking frequency, natural disasters, pests, freezing damage, and drought damage, which pose difficulties for the dynamic monitoring of yield. Moreover, judging the changes in tea plantations before and after picking by remote sensing images is more challenging due to the influence of weather conditions.
6 Conclusions and future trends
In this review, we have comprehensively summarized the latest developments in non-destructive techniques for numerous aspects of tea plant monitoring. Multispectral satellite images are frequently used in tea field-scale monitoring due to their capability to provide a broad range of data from tea gardens quickly and inexpensively. NIRS is the most used method for canopy-scale and leaf-scale monitoring of tea, likely due to the simple preprocessing procedure of NIRS data compared to complex imaging data. Significantly, many investigations have begun to focus on the in-situ detection of tea. Despite the expansive literature on non-invasive techniques and their impressive effectiveness, a compatible model with economical sensors is still absent for tea cultivation and production.
Comprehensive control of the climate conditions and growth status of tea plants is essential to provide basic support for precise regulation of tea plant growth. The combination of the above three scales monitoring, including tea garden identification, tea yield monitoring, tea pest and disease identification, tea quality determination, and tea plant classification, can present the growth trend of tea plants and the appearance of tea gardens in real-time. By predicting the occurrence of tea garden pests, diseases, and natural disasters, it provides data support for tea farming activities and tea garden regulation. Using integrating the data from the leaf, canopy, and tea garden scales, a tea quality detection model can be established. This model can predict tea quality status based on factors such as the growth environment, growth status, and expansion history of the tea plant, and provide corresponding recommendations and adjustment plans.
Apart from the techniques discussed in this review, some other techniques are currently adopted in the field. Raman spectroscopy[121] and nanosensor techniques are widely used to detect hazardous substances, pollutants, pesticide residues[122], and food additives, and have recently been applied to assess authenticity and adulteration in the tea industry. Indeed, these techniques have been used to classify tea types with varying levels of contamination or identify the degrees of adulteration, as well as other tea plant monitoring issues[37]. Moreover, material thermal imaging technology has been employed to measure the thickness and distribution of tea plants, leaf venation tendencies, and micro homogeneity by using the heat waves generated from the interior or surface of leaves. Thermal imaging technology can provide visual images of tea leaves, which can be utilized for the monitoring of tea leaves obtained from different cultivars. The benefits of using a combination of various analysis methods to detect tea plants in situ will be considerable compared to using one method alone. Furthermore, the use of portable apparatus and sensors that can be connected to smartphones is expected to increase soon. Additionally, the combination sensors of different scales, such as canopy scale and field scale, can complement each other to improve tea characteristics monitoring. It is anticipated that intelligent sensory technology combined with various sensors that can replicate the sensory judgments of tea experts will become a reality. We hope this review will furnish those working in tea fields with helpful information and will offer a beneficial and convenient resource for the in-situ and non-destructive observation of tea plants.
Acknowledgements
This work was financially supported by Chongqing Technology Innovation and Application Development Special Project (Grant No. cstc2021jscx-gksbX0064, CSTB2023TIAD-KPX0040, cstc2019 jscx-gksbX0092), National Key R&D Program (Grant No. 2021YFD1601103) of China.
Citation: Cao Q, Zhao C J, Xu Z, Jiang P, Yang H B, Meng X Y, et al. Review of the application of in-situ sensing techniques to address the tea growth characteristics from leaf to field. Int J Agric & Biol Eng, 2024; 17(1): 1-11.
Received date: 2023-06-26 Accepted date: 2023-11-05
Biographies: Qiong Cao, PhD candidate, research interest: agriculture information technology for tea, Email: [email protected]; Chunjiang Zhao, PhD, Researcher, research interest: smart agriculture, Email: zhaocj@nercita. org.cn; Ze Xu, MS, Researcher, research interest: tea production and processing technology, Email: [email protected]; Ping Jiang, PhD, Professor, research interest: agricultural electrification and automation, Email: 1233032@hunau. edu.cn; Haibin Yang, MS, Associate researcher, research interest: tea plant ecological cultivation, Email: [email protected]; Xiangyu Meng, MS, Research Associate, research interest: management of agricultural economy, Email: [email protected].
*Corresponding author: Guijun Yang, PhD, Researcher, research interest: agricultural quantitative remote sensing. Technology Research Center, Beijing Academy of Agriculture and Forestry, Beijing 100097, China. Tel: +86-10-51503647, Email: [email protected].
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Abstract
The tea plant is a valuable and evergreen crop that is extensively cultivated in China and many other countries. Currently, there is growing research interest in this plant. For the tea industry, it is crucial to develop rapid and non-invasive methods to evaluate tea plants in their natural environment. This article provides a comprehensive overview of non-invasive sensing techniques used for in-situ detection of tea plants. The topics covered include leaf, canopy, and field-level assessments, as well as statistical analysis techniques and characteristics specific to the research. Non-invasive testing technology is primarily used for monitoring and predicting tea pests and diseases, monitoring quality, and nutrients, determining tenderness and grade, identifying tea plant varieties, automatically detecting, and identifying tea buds, monitoring tea plant growth, and extracting tea garden areas through remote sensing. It also helps to evaluate planting suitability, assess disasters, and estimate yields. Additionally, the article examines the challenges and prospects of emerging techniques aimed at resolving the in-situ detection problem for tea plants. It can assist researchers and producers in comprehensively understanding the tea environment, quality characteristics, and growth process, thereby enhancing tea production quality, and fostering tea industry development.
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Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer
Details
1 Information Technology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
2 Tea Research Institute of Chongqing Academy of Agricultural Sciences, Chongqing 400000, China)
3 College of Machnical and Electrical Engineering, Hunan Agricultural University, Changsha 410125, China