Abstract/Details

Épidémiologie moléculaire d'un champignon causant la rouille des feuilles du peuplier, Melampsora medusae f. sp. deltoidae, en amérique du nord

Bourassa, Martin.  Universite Laval (Canada). ProQuest Dissertations Publishing, 2000. MQ51116.

Abstract (summary)

Because Melampsora medusae is an obligate parasite able to reproduce both sexually and asexually, two main sources may be considered as origin of primary inoculum in North America. Where the sympatry hosts are poplar (Populus deltoides) and larch (Larix laricina ), sexual reproduction is possible, and the primary inoculum is presumed to be ecidiospores produced on larch. The other potential source of primary inoculum is urediniospores produced through asexual reproduction on poplars. This source of inoculum could mainly originate from allopatry, where larch is absent.

Considering the hypothesis of two poles of origin, populations of sympatry should reveal a genetic structure reflecting the occurrence of sexual reproduction, that is an important genetic diversity and a low level of clonality, whereas in allopatry the reverse situation would be expected. Moreover, sympatry and allopatry should show the presence of distinct alleles.

To test this hypothesis, two polymorphic loci of M. medusae f. sp. deltoidae were identified by cloning. For each locus, one PCR-SSCP (Polymerase Chain Reaction - Single-Strand Conformational Polymorphism) marker was designed. (Abstract shortened by UMI.)

Indexing (details)


Subject
Molecular biology
Classification
0307: Molecular biology
Identifier / keyword
Biological sciences; French and English text
Title
Épidémiologie moléculaire d'un champignon causant la rouille des feuilles du peuplier, Melampsora medusae f. sp. deltoidae, en amérique du nord
Author
Bourassa, Martin
Number of pages
121
Degree date
2000
School code
0726
Source
MAI 39/01M, Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
978-0-612-51116-3
Advisor
Bernier, Louis
University/institution
Universite Laval (Canada)
University location
Canada
Degree
M.Sc.
Source type
Dissertation or Thesis
Language
French
Document type
Dissertation/Thesis
Dissertation/thesis number
MQ51116
ProQuest document ID
304655241
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
https://www.proquest.com/docview/304655241/abstract