New Methods for the Determination of Isoflavones From Soy and Derived Foods

Abstract

In this Doctoral Thesis several aspects of soy isoflavones determination were assessed for the development of new methods for the extraction, clean-up/concentration and hydrolysis, as well as new analysis methods by high performance chromatography methods. Previously, the stability of soy isoflavones extracts and standard solutions was evaluated. Thereafter, new extraction methods were developed using the latest techniques and materials available. It was evaluated the use of ultrasounds, pressurized liquids, microwaves and solid phase extraction for the extraction of soy isoflavones from several types of samples. The developed methods are fast, reliable and highly reproducible. New materials were also evaluated, like solid phase extraction polymeric cartridges and the monolithic columns for liquid chromatography. These materials were used for the determination of isoflavones from several soy juices samples with excellent results. Globally, the information provided allows increasing the speed, precision, reproducibility, flexibility and decreasing operational costs of the determination of isoflavones not only from soybeans, but also other matrices, since the developed methods can be potentially used for different types of samples.

Alternate abstract:

You are viewing a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer

Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer

En esta Tesis Doctoral se evaluaron varios aspectos de la determinación de isoflavonas de soja para el desarrollo de nuevos métodos de extracción, limpieza/concentración e hidrólisis, así como nuevos métodos de análisis por cromatografía de alta resolución. Previamente, se evaluó la estabilidad de extractos de isoflavonas de soja y soluciones estándar. A partir de entonces, se desarrollaron nuevos métodos de extracción utilizando las últimas técnicas y materiales disponibles. Se evaluó el uso de ultrasonidos, líquidos a presión, microondas y extracción en fase sólida para la extracción de isoflavonas de soja a partir de varios tipos de muestras. Los métodos desarrollados son rápidos, fiables y altamente reproducibles. También se evaluaron nuevos materiales, como los cartuchos poliméricos de extracción en fase sólida y las columnas monolíticas para cromatografía líquida. Estos materiales se utilizaron para la determinación de isoflavonas a partir de varias muestras de jugos de soya con excelentes resultados. A nivel global, la información proporcionada permite aumentar la velocidad, precisión, reproducibilidad, flexibilidad y disminuir los costos operativos de la determinación de isoflavonas no solo de soja, sino también de otras matrices, ya que los métodos desarrollados pueden ser potencialmente utilizados para diferentes tipos de muestras.