Abstract/Details

Identification de séquences génomiques avoisinant un gène impliqué dans la pathogénécité et protéines candidates pouvant être codées par cette région, chez le champignon Ophiostoma novo-ulmi

Majeau, Josee-Anne.  Universite Laval (Canada). ProQuest Dissertations Publishing, 2005. MR14733.

Abstract (summary)

The understanding of the molecular mechanisms implicated in symptom development of Dutch elm disease is of great interest for biological control of Ophiostoma novo-ulmi, the principal agent of Dutch elm disease. In this respect, the identification of virulence genes in this fungus is of prime importance. Pat1 is the only pathogenicity locus reported so far and its involvement in the virulence still needs to be confirmed. We studied genes and proteins that might be encoded by Pat1 by two different methods: chromosome walking on the aggressive strain H327 DNA and 2D gel electrophoresis of yeast-like cells. Several open reading frames were sequenced during walking including one which was coding for a high affinity ammonium transporter. In addition, we also found ten candidate peptides for Pat1 and another one for the MatA mating-type allele. Additional tests are needed to confirm the identity of Pat1 but we have provided the first genomic description of the genes located in the vicinity of the Pat1 locus and identified peptides that could be encoded by it.

Indexing (details)


Subject
Plant pathology
Classification
0480: Plant pathology
Identifier / keyword
Biological sciences
Title
Identification de séquences génomiques avoisinant un gène impliqué dans la pathogénécité et protéines candidates pouvant être codées par cette région, chez le champignon Ophiostoma novo-ulmi
Author
Majeau, Josee-Anne
Number of pages
124
Degree date
2005
School code
0726
Source
MAI 44/06M, Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
978-0-494-14733-7
University/institution
Universite Laval (Canada)
University location
Canada
Degree
M.Sc.
Source type
Dissertation or Thesis
Language
French
Document type
Dissertation/Thesis
Dissertation/thesis number
MR14733
ProQuest document ID
305364990
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
https://www.proquest.com/docview/305364990/abstract