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This study delves into analyzing the effects of various machining techniques on the flexural fatigue life of Ti-6Al-4V L-PBF specimens. The fatigue life and fracture behavior of specimens subjected to milling, grinding, polishing, and abrasive media blasting were compared. The findings reveal significant differences in the fatigue resistance between machined and non-machined parts. This study contributes to the understanding of the effects of post-processing on the durability of L-PBF manufactured components, offering insights for enhancing their application in critical aerospace and biomedical applications.