Correction: Zhao et al. Hypermethylation of UCHL1 Promotes Metastasis of Nasopharyngeal Carcinoma by Suppressing Degradation of Cortactin (CTTN). Cells 2020, 9, 559

Error in Figure

In the original publication [1], there were mistakes in Figures 3 and 6 as published. Incorrect images were used for the invasion-SUNE1-shcon group in Figure 3F, migration-SUNE1-shUCHL1+C90S group in Figure 6A, invasion-HONE1-Vec+Vec and UCHL1+CTTN groups in Figure 6C, and the migration-SUNE1-UCHL1+CTTN group in Figure 6D. These errors have been corrected. The corrected Figure 3 and Figure 6 appear below. The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.

Footnotes

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Figures

Figure 3. UCHL1 inhibits the migration and invasion of NPC cells in vitro. (A) qRT-PCR analysis of UCHL1 mRNA (up) and immunoblot analysis of UCHL1 and α-tubulin (down) in SUNE1 and HONE1 cells transfected with empty vector or plasmid encoding UCHL1. (B,C) Cell migration was measured using a wound healing assay (×200) (B) and transwell assay (×200) without Matrigel (C). Invasion was measured using a transwell assay with Matrigel (×200) (C). (D) qRT-PCR analysis of UCHL1 mRNA (up) and immunoblot analysis of UCHL1 and α-tubulin (down) in SUNE1 and HONE1 cells transfected with control or shUCHL1(#1 and #2) that stably overexpressed UCHL1. (E–H) Cell migration was measured using a wound healing assay (E) and transwell assay without Matrigel (F,G). Invasion was measured using a transwell assay with Matrigel (F,H). Scale bar:100 µm; Mean ± S.D.; * p < 0.05, ** p < 0.01; *** p < 0.001; Student’s t-tests. Data are representative of at least three independent experiments.

Figure 6. CTTN is a functional and major target of UCHL1 in NPC. (A,B) Transwell assay performed with SUNE1 (A) or HONE1 (B) cells stably transfected with control or shUCHL1 and reconstructed with vector, UCHL1, or UCHL1(C90S) in the presence (invasion) or absence (migration) of Matrigel. (C,D) Transwell assay performed with SUNE1 (C) or HONE1 (D) cells that stably transfected with vector or UCHL1 with reconstructed with vector or CTTN in the presence (invasion) or absence (migration) of Matrigel. Scale bar: 100 µm; Mean ± S.D.; * p < 0.05, ** p < 0.01, *** p < 0.001, NS: no significance compared with vector; Student’s t-tests. Data are representative of at least three independent experiments.

Reference

1. Zhao, Y.; Lei, Y.; He, S.-W.; Li, Y.-Q.; Wang, Y.-Q.; Hong, X.-H.; Liang, Y.-L.; Li, J.-Y.; Chen, Y.; Luo, W.-J. et al. Hypermethylation of UCHL1 Promotes Metastasis of Nasopharyngeal Carcinoma by Suppressing Degradation of Cortactin (CTTN). Cells; 2020; 9, 559. [DOI: https://dx.doi.org/10.3390/cells9030559]