Accuracy of sperm velocity assessment using the Sperm Quality Analyzer V

Abstract

Aim:  The correct diagnosis of the functional capacity of human sperm is limited. The Sperm Quality Analyzer (SQA) with the visualization system (SQA V, Medical Electronic System, Hatavorzo, Israel), an upgraded version of SQA, was recently developed to provide a rapid and low-cost quantitative evaluation of sperm quality as well as sperm velocity assessment. The aim of the present study was to evaluate whether the SQA V's new parameters correlate with computer-aided sperm analysis (CASA) estimates.

Methods:  Semen quality analysis of 66 fresh samples was determined using SQA V and CASA.

Results:  There were significant correlations of total sperm concentration (P < 0.001), sperm motility (P = 0.145), and percentage of progressive motile sperm (P = 0.001), between the SQA V variables and the CASA estimates. The sperm velocity assessed by SQA V was significantly correlated with some of the CASA estimates, including sperm motility (P = 0.001), the percentage of progressively motile sperm (P < 0.001), straight-line velocity (P < 0.001), curvilinear velocity (P < 0.001) and average path velocity (P < 0.001). However, it did not correlate with amplitude of lateral head displacement, beat cross frequency, straightness, or linearity, assessed by CASA.

Conclusion:  Assessment of sperm motility has been shown as one of the important factors to predict the functional capacity of human sperm. On the basis of the present study, SQA V is considered useful for screening sperm quality in the management of male infertility. (Reprod Med Biol 2003; 2: 151–157)

REFERENCES 1 Donnelly ET, Lewis SE, McNally JA, Thompson W. In vitro fertilization and pregnancy rates: the influence of sperm motility and morphology on IVF outcome. Fertil Steril 1998; 70: 305–314. 2 Mahadevan MM, Trounson AO. The influence of seminal characteristics on the success rate of human in vitro fertilization. Fertil Steril 1984; 42: 400–405. 3 Dunphy BC, Neal LM, Cooke ID. The clinical value of conventional semen analysis. Fertil Steril 1989; 51: 324–329. 4 Silber SJ. The relationship of abnormal semen parameters to male fertility. Hum Reprod 1989; 4: 947–953. 5 Yeung CH, Cooper TG, Nieschlag E. A technique for standardization and quality control of subjective sperm motility assessments in semen analysis. Fertil Steril 1997; 67: 1156–1158. 6 Chong AP, Walters CA, Weinrieb SA. The neglected laboratory test. The semen analysis. J Androl 1983; 4: 280–282. 7 Jequier AM, Ukombe EB. Errors inherent in the performance of a routine semen analysis. Br J Urol 1983; 55: 434–436. 8 Mortimer D, Shu MA, Tan R. Standardization and quality control of sperm concentration and sperm motility counts in semen analysis. Hum Reprod 1986; 1: 299–303. 9 Dunphy BC, Kay R, Barratt CL, Cooke ID. Quality control during the conventional analysis of semen, an essential exercise. J Androl 1989; 10: 378–385. 10 Neuwinger J, Behre HM, Nieschlag E. External quality control in the andrology laboratory: an experimental multicenter trial. Fertil Steril 1990; 54: 308–314. 11 Cooper TG, Atkinson AD, Nieschlag E. Experience with external quality control in spermatology. Hum Reprod 1999; 14: 765–769. 12 Holt W, Watson P, Curry M, Holt C. Reproducibility of computer-aided semen analysis: comparison of five different systems used in a practical workshop. Fertil Steril 1994; 62: 1277–1282. 13 Davis RO, Katz DF. Standardization and comparability of CASA instruments. J Androl 1992; 13: 81–86. 14 Parinaud J, Richoilley G, Moutaffian H et al. Are the characteristics of spermatozoa in the insemination medium useful for predicting in-vitro fertilization results? Int J Androl 1996; 19: 103–108. 15 Hirano Y, Shibahara H, Obara H et al. Relationships between sperm motility characteristics assessed by the computer-aided sperm analysis (CASA) and fertilization rates in vitro. J Assist Reprod Genet 2001; 18: 213–218. 16 Bartoov B, Ben-Barak J, Mayevsky A, Sneider M, Yogev L, Lightman A. Sperm motility index: a new parameter for human sperm evaluation. Fertil Steril 1991; 56: 108–112. 17 Martinez C, Mar C, Azcarate M, Pascual P, Aritzeta JM, Lopez-Urrutia A. Sperm motility index: a quick screening parameter from sperm quality analyser-IIB to rule out oligo- and asthenozoospermia in male fertility study. Hum Reprod 2000; 15: 1727–1733. 18 Shibahara H, Suzuki T, Obara H et al. Accuracy of the normal sperm morphology value by Sperm Quality Analyzer IIC: comparison with the strict criteria. Int J Androl 2002; 25: 45–48. 19 Shibahara H, Naito S, Hasegawa A, Mitsuo M, Shigata M, Koyama K. Evaluation of sperm fertilizing ability using the Sperm Quality Analyzer. Int J Androl 1997; 20: 112–117. 20 Cates W, Farley TM, Rowe PJ. Worldwide patterns of infertility: is Africa different? Lancet 1985; 14: 596–598. 21 World Health Organization. WHO Laboratory Manual for the Examination of Human Sperm and Semen–Cervical Mucus Interaction, 4th edn. Cambridge: Cambridge University Press, 1999. 22 Aitken RJ, Sutton M, Warner P, Richardson DW. Relationship between the movement characteristics of human spermatozoa and their ability to penetrate cervical mucus and zona-free hamster oocytes. J Reprod Fertil 1985; 73: 441–449. 23 Irvine DS, Aitken RJ. Predictive value of in-vitro sperm function tests in the context of an AID service. Hum Reprod 1986; 1: 539–545. 24 Bostofte E, Bagger P, Michael A, Stakemann G. Fertility prognosis for infertile men: results of follow-up study of semen analysis in infertile men from two different populations evaluated by the Cox regression model. Fertil Steril 1990; 54: 1100–1106. 25 Shibahara H, Mitsuo M, Inoue M, Hasegawa A, Shigeta M, Koyama K. Relationship between human in-vitro fertilization and intracytoplasmic sperm injection and the zona-free hamster egg penetration test. Hum Reprod 1998; 13: 1928–1932. 26 ESHRE Andrology Special Interest Group. Consensus workshop on advanced diagnostic andrology techniques. Hum Reprod 1996; 11: 1463–1479. 27 Burkman LJ, Coddington CC, Franken DR et al. The hemizona assay (HZA): development of a diagnostic test for the binding of human spermatozoa to the human hemizona pellucida to predict fertilization potential. Fertil Steril 1988; 49: 688–697. 28 Overstreet JW, Katz DF, Hanson FW, Fonseca JR. A simple inexpensive method for objective assessment of human sperm movement characteristics. Fertil Steril 1979; 31: 162–172. 29 Katz DF, Overstreet JW. Sperm motility assessment by videomicrography. Fertil Steril 1981; 35: 188–193. 30 Mathur S, Carlton M, Ziegler J, Rust FP, Williamson HO. A computerized sperm motion analysis. Fertil Steril 1986; 46: 484–488. 31 Beauchamp PJ, Galle PC, Blasco L. Human sperm velocity and postinsemination cervical mucus test in the evaluation of the infertile couple. Arch Androl 1984; 13: 107–112. 32 Fetterolf PM, Rogers BJ. Prediction of human sperm penetrating ability using computerized motion parameters. Mol Reprod Dev 1990; 27: 326–331. 33 Suzuki T, Shibahara H, Tsunoda H et al. Comparison of the Sperm Quality Analyzer IIC variables with the computer-aided sperm analysis estimates. Int J Androl 2002; 2: 49–54.