Ethanol production from two varieties of henequen (Agave fourcroydes Lem)

Abstract

This work demonstrates the significant differences in ethanol production of two henequen (Agave fourcroydes Lem) varieties. Yaax ki, or green henequen, surpasses Sac ki, or white henequen, in weight, sugar accumulation capability and ethanol production. The study was carried out with the ‘piña’ (stem and basal part of the leaves that remain attached to it after harvesting the leaves) of 5- and 9-year-old plants, cultivated in two localities of Yucatán, México. At 5 years of age, Yaax ki piñas are 33% larger than those of Sac ki, and at 9 years this difference can increase to 59%. Juice from 5-year-old piñas of Yaax ki contains 15.6% more reducing sugars, whereas in the must it can exceed 67%. Values obtained in the 9-year-old plants indicate that the Yaax ki variety accumulates 30.6% more reducing sugars than the Sac ki, whereas in the must the difference is in favor of Yaax ki is 21.7%. To produce 1 L of ethanol at 40% concentration by volume from 5-year-old plants, 48 kg of Sac ki piñas and 29 kg of Yaax ki are required, whereas with the 9-year-old plants, only 23 and 19 kg are needed, respectively.

References Aspinall GO, Gupta PC (1959) The structure of the fructosan from Agave veracruz Mili. Journal of American Chemistry Society, 81, 718–722. Barnett JA, Payne RW, Yarrow D (2001) Yeast: Characteristics and Identification, 3rd edn. Cambridge University Press, Cambridge, UK. Cáceres-Farfán M, Lappe P, Larqué-Saavedra A, Magdub-Méndez A, Barahona-Pérez F (2008) Ethanol production from henequen (Agave fourcroydes Lem.) juice and molasses by a mixture of two yeasts. Bioresource Technology, 99, 9036–9039. Central Bureau voor Schimmel Culture (CBS Database) Available at: http://www.cbs.knaw.nl/databases/ (accessed 8 February 2002). Cedeño MC, Alvarez-Jacobs J (2000) Tequila production from Agave. In: The Alcohol Textbook, 3rd edn (eds Murtagh & Associates), pp. 223–243. Nottingham University Press, Nottingham, UK. Colunga-GarcíaMarín P (2003) The domestication of henequén (Agave fourcroydes Lem.). In: The Lowland Maya Area. Three Millennia at the Human–Wildland Interface (eds Gómez-Pompa A, Allen MF, Fedick SL, Jiménez-Osornio JJ), pp. 439–446. The Haworth Press Inc., Binghampton, NY. Colunga-GarcíaMarín P, Larqué-Saavedra A, Eguiarte L, Zizumbo-Villarreal D (2007) En lo Ancestral hay futuro: Del tequila, los mezcales y otros agaves. CICY-CONACYT-CONABIO-INE, México, 402 pp. Colunga-GarciaMarín P, May-Pat F (1993) Agave studies in Yucatan, Mexico I. past and present germplasm diversity and uses. Economic Botany, 47, 312–327. Duch GJ (1988) La Conformación Territorial del Estado de Yucatán. Universidad Autónoma de Chapingo. Estado de México. México, 180 pp. Duch GJ (1991) Fisiografía del Estado de Yucatán, su Relación con la Agricultura. Universidad Autónoma de Chapingo. Estado de México. México, 223 pp. García-AIbornoz MA (2006) Determinación y caracterización de fructanos provenientes de Henequén (Agave fourcroydes Lem.). MSc thesis. Centro de Investigación Científica de Yucatán A.C. México, 45 pp. Gómez-Ayala RC, Jacques C, Ramírez-de León JA (2004) Extracción de la inulina y azúcares del agave con métodos químicos. Revista Salud Pública y Nutrición (RESPYN) No. 1. Ed. Especial. Disponible en. Available at: http://www.uanl.mx/publicaciones/respynlespeciales/ee-I-2005/5I.htlm (Diciembre de 2006). Gough S, Flynn O, Hack CJ, Marchant R (1996) Fermentation of molasses using a thermotolerant yeast, Kluyveromyces meuxianus IMB3; simplex optimization of media supplements. Applied Microbiology and Biotechnology, 46, 187–190. Hall T (2005) BioEdit. Available at: http://www.mbio.ncsu.edu/BioEdit/bioedit.html (accessed 18 March 2005). Kourkoutas Y, Dimitropoulou S, Kenallaki M (2002) High-temperature alcoholic fermentation of whey using Kluyveromyces marxianus 1MB3 yeast immobilized on delignified cellulosic material. Bioresource Technology, 82, 177–181. Kurtzman CP, Fell JW (1998) The Yeasts. A Taxonomic Study, 4th edn. Elsevier, Amsterdam, the Netherlands. Lappe P, Ulloa M, Arce-Rocha G, Cáceres-Farfán M, Tapia-Tussell R, Pérez-Brito D, Larqué-Saavedra A (2004) Isolation and identification of the mycobiota present in Agave fourcroydes. Eleventh International Congress on Yeasts. Yeasts in Science and Technology. The Quest for Sustainable Development, Rio de Janeiro, Brazil, 173 pp. Larqué-Saavedra A, Magdub-Méndez A, Cáceres-Farfán M (2004) Proceso para la fabricación de bebida alcohólica a partir del henequén (Agave fourcroydes). Centro de Investigación Científica de Yucatán, A.C. Mexico. Patent 219235. López MG, Mancilla-Margalli NA, Mendoza-Díaz G (2003) Molecular structures of fructans from Agave tequilana Weber var. azul. Journal of Agricultural Food Chemistry, 51, 7835–7840. Miller LG (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugars. Analytical Chemistry, 31, 4226–4228. Nobel PS (1985) PAR, water and temperature limitations on the productivity of cultivated Agave fourcroydes (henequen). Journal of Applied Ecology, 22, 157–173. Pérez-Brito D, Tapia-Tussell R, Quijano-Ramayo A, Larqué-Saavedra A, Lappe P (2007) Molecular characterization of Kluyveromyces marxianus strains isolated from Agave fourcroydes Lem in Yucatán, México. Molecular Biotechnology, 37, 181–186. Rendón-Salcido LA, Colunga-GarcíaMarín P, Barahona-Pérez LF, Pimienta-Barrios E, Magdub-Méndez A, Larqué Saavedra A (2009) Sugars and alcoholic byproducts from henequén (Agave fourcroydes) as influenced by plant age and climate. Revista Fitotecnia Mexicana, 33, 39–44. Segura-García LE (2010) Identificación y caracterización por métodos moleculares de levaduras aisladas del proceso de elaboración del mezcal en el estado de Oaxaca. MSc thesis. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Guadalajara, México, 163 pp. Tapia-Tussell R, Lappe P, Ulloa M, Quijano-Ramayo A, Cáceres-Farfán M, Larqué Saavedra A, Pérez-Brito D (2006) A rapid and simple method for DNA extraction from yeast and fungi isolated from Agave fourcroydes. Molecular Biotechnology, 33, 67–70. Tzec-Gamboa MC (2006) Evaluación de levaduras aisladas de mostos de henequén (Agave fourcroydes) para la producción de una bebida alcohólica. MSc thesis. Centro de Investigación Científica de Yucatán A.C. Mérida, México, 74 pp. Wang N, Nobel PS (1998) Phloem transport of fructans in the crassulacean acid metabolism species Agave deserti. Plant Physiology, 116, 709–714. Williams M, Reese D (1950) Colorimetric determination of ethyl alcohol. Analytical Chemistry, 22, 1556–1561. Yarrow D (1998) Chapter II. Methods for isolation, maintenance and identification of yeasts. In: The Yeasts. A Taxonomic Study, 4th edn (eds Kurtzman CP, Fell JW), pp. 76–100. Elsevier, Amsterdam, the Netherlands.