Abstract

Members of the Metal Tolerance Protein (MTP) family are critical in mediating the transport and tolerance of divalent metal cations. Despite their significance, the understanding of MTP genes in mustard (Brassica juncea) remains limited, especially regarding their response to heavy metal (HM) stress. In our study, we identified MTP gene sets in Brassica rapa (17 genes), Brassica nigra (18 genes), and B. juncea (33 genes) using the HMMER (Cation_efflux; PF01545) and BLAST analysis. For the 33 BjMTPs, a comprehensive bioinformatics analysis covering the physicochemical properties, phylogenetic relationships, conserved motifs, protein structures, collinearity, spatiotemporal RNA-seq expression, GO enrichment, and expression profiling under six HM stresses (Mn2+, Fe2+, Zn2+, Cd2+, Sb3+, and Pb2+) were carried out. According to the findings of physicochemical characteristics, phylogenetic tree, and collinearity, the allopolyploid B. juncea’s MTP genes were inherited from its progenitors, B. rapa and B. nigra, with minimal gene loss during polyploidization. Members of the BjMTP family exhibited conserved motifs, promoter elements, and expression patterns across subgroups, consistent with the seven evolutionary branches (G1, G4–G9, and G12) of the MTPs. Further, spatiotemporal expression profiling under HM stresses successfully identified specific genes and crucial cis-regulatory elements associated with the response of BjMTPs to HM stresses. These findings may contribute to the genetic improvement of B. juncea for enhanced HM tolerance, facilitating the remediation of HM-contaminated areas.

Details

Title
Molecular characterization and expression patterns of MTP genes under heavy metal stress in mustard (Brassica juncea L.)
Author
You, Liang 1 ; Sheng, Jialin 1 ; Jiang, Guoxiang 1 ; Chen, Hao 2 ; Yuan, Yuhui 1 ; Gong, Sha 1 ; Yan, Mingli 3 ; Hu, Junhe 1 ; Xiang, Guohong 1 ; Duan, Renyan 1 ; Chen, Yong 1 ; Liu, Xianjun 1 

 Hunan University of Humanities, Science and Technology, College of Agriculture and Biology, Key Laboratory of Development and Utilization and Quality and Safety Control of Characteristic Agricultural Resources in Central Hunan of College of Hunan Province, Loudi, China (GRID:grid.440781.e) (ISNI:0000 0004 1759 997X) 
 Hunan Agricultural University, College of Agronomy, Changsha, China (GRID:grid.257160.7) (ISNI:0000 0004 1761 0331) 
 Hunan Academy of Agricultural Sciences, Crop Research Institute, Changsha, China (GRID:grid.410598.1) (ISNI:0000 0004 4911 9766) 
Pages
17857
Publication year
2024
Publication date
2024
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3087040318
Copyright
© The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.