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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Chronic pancreatitis (CP) in young individuals may lead to disease-related secondary sarcopenia (SSARC), characterized by muscle loss and systemic inflammation. In this study, CP was induced in young pigs, and serum levels of key hormones, muscle fiber diameters in various muscles, and the mRNA expression of genes related to oxidative stress and programmed cell death were assessed. A decrease in muscle fiber diameters was observed in SSARC pigs, particularly in the longissimus and diaphragm muscles. Hormonal analysis revealed alterations in dehydroepiandrosterone, testosterone, oxytocin, myostatin, and cortisol levels, indicating a distinct hormonal response in SSARC pigs compared to controls. Oxytocin levels in SSARC pigs were significantly lower and myostatin levels higher. Additionally, changes in the expression of catalase (CAT), caspase 8 (CASP8), B-cell lymphoma 2 (BCL2), and BCL2-associated X protein (BAX) mRNA suggested a downregulation of oxidative stress response and apoptosis regulation. A reduced BAX/BCL2 ratio in SSARC pigs implied potential caspase-independent cell death pathways. The findings highlight the complex interplay between hormonal changes and muscle degradation in SSARC, underscoring the need for further research into the apoptotic and inflammatory pathways involved in muscle changes due to chronic organ inflammation in young individuals.

Details

Title
Understanding Secondary Sarcopenia Development in Young Adults Using Pig Model with Chronic Pancreatitis
Author
Tomaszewska, Ewa 1   VIAFID ORCID Logo  ; Wojtysiak, Dorota 2   VIAFID ORCID Logo  ; Grzegorzewska, Agnieszka 3 ; Świątkiewicz, Małgorzata 4   VIAFID ORCID Logo  ; Donaldson, Janine 5   VIAFID ORCID Logo  ; Arciszewski, Marcin B 6   VIAFID ORCID Logo  ; Dresler, Sławomir 7   VIAFID ORCID Logo  ; Puzio, Iwona 1   VIAFID ORCID Logo  ; Szymańczyk, Sylwia 1   VIAFID ORCID Logo  ; Dobrowolski, Piotr 8   VIAFID ORCID Logo  ; Bonior, Joanna 9   VIAFID ORCID Logo  ; Mielnik-Błaszczak, Maria 10 ; Kuc, Damian 10 ; Muszyński, Siemowit 11   VIAFID ORCID Logo 

 Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-950 Lublin, Poland; [email protected] (I.P.); [email protected] (S.S.) 
 Department of Animal Genetics, Breeding and Ethology, Faculty of Animal Sciences, University of Agriculture in Kraków, 30-059 Kraków, Poland; [email protected] 
 Department of Animal Physiology and Endocrinology, University of Agriculture in Kraków, 30-059 Kraków, Poland; [email protected] 
 Department of Animal Nutrition and Feed Science, National Research Institute of Animal Production, 32-083 Balice, Poland; [email protected] 
 School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Parktown, Johannesburg 2193, South Africa; [email protected] 
 Department of Animal Anatomy and Histology, University of Life Sciences in Lublin, 20-950 Lublin, Poland; [email protected] 
 Department of Analytical Chemistry, Medical University of Lublin, 20-093 Lublin, Poland; [email protected]; Department of Plant Physiology and Biophysics, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, 20-033 Lublin, Poland 
 Department of Functional Anatomy and Cytobiology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, 20-033 Lublin, Poland; [email protected] 
 Department of Medical Physiology, Chair of Biomedical Sciences, Institute of Physiotherapy, Faculty of Health Sciences, Jagiellonian University Medical College, 31-501 Kraków, Poland; [email protected] 
10  Chair and Department of Developmental Dentistry, Medical University of Lublin, 20-081 Lublin, Poland; [email protected] (M.M.-B.); [email protected] (D.K.) 
11  Department of Biophysics, Faculty of Environmental Biology, University of Life Sciences in Lublin, 20-950 Lublin, Poland; [email protected] 
First page
8735
Publication year
2024
Publication date
2024
Publisher
MDPI AG
ISSN
16616596
e-ISSN
14220067
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3097940593
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.