Abstract

Background: Oral microbiota is believed to play important roles in systemic diseases, including cancer.Methods: We collected oral samples (tongue, buccal, supragingival, and saliva) and pancreatic tissue or intestinal samples from 52 subjects, and characterized 16S rRNA genes using high-throughput DNA sequencing.Results: Bray–Curtis plot showed clear separations between bacterial communities in the oral cavity and those in intestinal and pancreatic tissue samples. PERMANOVA tests indicated that bacterial communities from buccal samples were similar to supragingival and saliva samples, and pancreatic duct samples were similar to pancreatic tumor samples, but all other samples were significantly different from each other. A total of 73 unique Amplicon Sequence Variants (ASVs) were shared between oral and pancreatic or intestinal samples. Only four ASVs showed significant concordance, and two specific bacterial species (Gemella morbillorum and Fusobacterium nucleatum subsp. vincentii) showed consistent presence or absence patterns between oral and intestinal or pancreatic samples, after adjusting for within-subject correlation and disease status. Lastly, microbial co-abundance analyses showed distinct strain-level cluster patterns among microbiome members in buccal, saliva, duodenum, jejunum, and pancreatic tumor samples.Conclusions: Our findings indicate that oral, intestinal, and pancreatic bacterial microbiomes overlap but exhibit distinct co-abundance patterns in patients with pancreatic cancer and other gastrointestinal diseases.

Details

Title
Comparisons of oral, intestinal, and pancreatic bacterial microbiomes in patients with pancreatic cancer and other gastrointestinal diseases
Author
Chung, Mei 1   VIAFID ORCID Logo  ; Zhao, Naisi 1   VIAFID ORCID Logo  ; Meier, Richard 2 ; Koestler, Devin C 3   VIAFID ORCID Logo  ; Wu, Guojun 4 ; Erika del Castillo 5 ; Paster, Bruce J 6 ; Charpentier, Kevin 7 ; Izard, Jacques 8 ; Kelsey, Karl T 9 ; Michaud, Dominique S 1   VIAFID ORCID Logo 

1  Department of Public Health and Community Medicine, School of Medicine, Tufts University, Boston, MA, USA 
2  Department of Biostatistics, University of Kansas Medical Center, Kansas City, KS, USA 
3  Department of Biostatistics, University of Kansas Medical Center, Kansas City, KS, USA; University of Kansas Cancer Center, The University of Kansas Medical Center, Kansas City, KS, USA 
4  Department of Biochemistry and Microbiology, Center for Nutrition, Microbiome and Health, New Jersey Institute for Food, Nutrition and Health, Rutgers University, New Brunswick, NJ, USA 
5  Department of Microbiology, The Forsyth Institute, Cambridge, MA, USA 
6  Department of Microbiology, The Forsyth Institute, Cambridge, MA, USA; Department of Oral Medicine, Infection & Immunity, Harvard School of Dental Medicine, Boston, MA, USA 
7  Department of Surgery, Rhode Island Hospital, Providence, RI, USA 
8  Department of Food Science and Technology, University of Nebraska, Lincoln, NE, USA; Fred and Pamela Buffet Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA 
9  Center for Environmental Health and Technology, Brown University, Providence, RI, USA 
Publication year
2021
Publication date
Dec 2021
Publisher
Taylor & Francis Ltd.
e-ISSN
20002297
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1080/20002297.2021.1887680
ProQuest document ID
3100768506
Copyright
© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.