Abstract

Background

This research aims to determine the probable protective effect of nano-curcumin (N-CUR) on caffeine (1,3,7-trimethylxanthine)-induced neurotoxicity in cerebral rats.

Methods

Twenty-four male Wistar rats were divided into three groups: control, caffeine (150 mg kg−1), and caffeine (150 mg kg−1) treated with N-CUR (300 mg kg−1). All treatments were administrated by gavage every day for a month.

Results

Administration of caffeine significantly elevated the levels of serum interleukins 6 (IL-6), tumor necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF), and cyclooxygenase2 (COX-2). Also, there was a significant increase in levels of cerebral malondialdehyde (MDA), significantly diminished glutathione (GSH), and superoxide dismutase (SOD) activity. Caffeine administration significantly downregulated the gene expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulated the expression of nuclear factor kappa-light-chain enhancer of activated B cells (NF-κB). Administration of N-CUR caused a significant amelioration in TNF-α and IL-6 levels and a significant rise in SOD activity, while it caused a significant downregulation in NF-κB mRNA expression. Additionally, N-CUR has exerted insignificant amelioration of COX-2 and MDA contents and Nrf2 mRNA expression compared to the caffeine-treated group.

Conclusion

N-CUR may have a mild to moderate ameliorative effect on caffeine-induced apoptosis, oxidative stress, and inflammatory response in the cerebrum.

Highlights

Caffeine induced oxidative stress, apoptosis, and inflammation in the cerebrum.

Caffeine resulted in significantly increased COX-2 and VEGF levels.

Caffeine increased the expression of NF-ĸB and inhibited Nrf2 in cerebrum.

N-CUR may have a mild to moderate ameliorative effect on caffeine exposure.

Humans could confine caffeine intake to evade any alterations in the cerebrum.

Details

Title
Nano-Curcumin improves caffeine-induced cerebral alterations in male Wistar rats by modifying oxidative stress, inflammation, and COX-2/NF-κB/Nrf2 signaling
Author
Morsy, Moustafa M. 1 ; Ahmed, R. G. 2   VIAFID ORCID Logo  ; Abdel-Gabbar, Mohammed 1 

1  Beni-Suef University, Biochemistry Department, Faculty of Science, Beni-Suef, Egypt (GRID:grid.411662.6) (ISNI:0000 0004 0412 4932) 
2  Beni-Suef University, Zoology Department, Faculty of Science, Beni-Suef, Egypt (GRID:grid.411662.6) (ISNI:0000 0004 0412 4932) 
Pages
93
Publication year
2024
Publication date
Dec 2024
Publisher
Springer Nature B.V.
ISSN
23148535
e-ISSN
23148543
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/s43088-024-00526-5
ProQuest document ID
3108458093
Copyright
© The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.