Content area
Background
Large-scale HIV genotype drug resistance study has not been conducted in Chongqing.
Methods
A retrospective study was conducted on people living with HIV(PLWH) who received HIV-1 genotype resistance testing at Chongqing Public Health Medical Center from May 2016 to June 2023. The HIV-1pol gene was amplified through RT-PCR and analyzed in terms of genotypic drug resistance.
Results
Of the 3015 PLWH tested for HIV-1 drug resistance, 1405 (46.6%) were resistant to at least one antiviral drug. Among non-nucleoside reverse transcriptase inhibitors (NNRTIs), 43.8% were resistant, compared to 29.5% for nucleoside reverse transcriptase inhibitors (NRTIs) and 3.4% for protease inhibitors (PIs). V179D/E and K103N/S were identified as the common mutation sites in the NNRTIs class of drugs, M184V/I and K65R/N were reported as the most common mutation sites in NRTIs, while thymidine analogue mutation (TAM) group was identified in 373 samples. L10FIV was the most common mutation in PIs. The dominant HIV-1 subtype was CRF07_BC.
Conclusions
The high prevalence of HIV-1 drug resistance in Chongqing underscores the imperative for rigorous surveillance of the local HIV epidemic. Furthermore, TAMs are associated with HIV-1 multidrug resistance, and timely detection of drug resistance is helpful to reduce the emergence and spread of such drug-resistant strains.
Details
Public health;
Mutation;
Drug resistance;
Proteinase inhibitors;
Polymerase chain reaction;
Genotypes;
Gene amplification;
Antiretroviral drugs;
Genotype & phenotype;
Multidrug resistance;
Antiviral agents;
Non-nucleoside reverse transcriptase inhibitors;
Protease inhibitors;
Health care facilities;
Human immunodeficiency virus--HIV;
Nucleosides;
Thymidine;
RNA-directed DNA polymerase;
Nucleoside reverse transcriptase inhibitors;
Chromosome 1;
GLP-1 receptor agonists;
Antiviral drugs