Content area
Vimentin is a type III intermediate filament (IF) protein, that is induced in a large number of solid tumours. A single cysteine at position 328 in vimentin plays a crucial role in assembly, organisation and stability of IFs. However, its exact function during epithelial mesenchymal transition (EMT) and cancer progression has not been investigated. To investigate this, we have transduced wildtype (WT) and C328S vimentin separately in MCF-7 cells that lack endogenous vimentin. The expression of C328-VIM impacted vimentin-actin interactions and induced EMT-like features that include enhanced cell proliferation, migration, invasion accompanied by reduced cell adhesion when compared to the wildtype cells. Functional transcriptomic studies confirmed the upregulation of EMT and mesenchymal markers, downregulation of epithelial markers as well as acquisition of signatures associated with cancer stemness (CD56, Oct4, PROCR and CD49f) thus transforming MCF-7 cells from oestrogen positive to triple reduced (ESR1, PGR, and HER2) status. We also observed a stark increase in the expression of long non-coding RNA, XIST in MCF-7 cells expressing C328-VIM. Targeting the mutant vimentin or XIST by RNA interference partially reversed the phenotypes in C328-VIM expressing MCF-7 cells. Furthermore, introduction of C328-VIM cells into nude mice promoted tumour growth by increasing cancer stemness in an oestrogen independent manner. Altogether, our studies provide insight into how cysteine 328 in vimentin dictates mechano-transduction signals to remodel actin cytoskeleton and protect against EMT and cancer growth via modulating lncRNA XIST. Therefore, targeting vimentin and/or XIST via RNA interference should be a promising therapeutic strategy for breast cancer treatment.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
* Five major changes have been introduced in this manuscript. i. A graphical abstract has been added to explain the content to readers without going into details. 2.Figures have been expanded to increase visibility. 3. New text has been added on pages 16/17 (Results) to explain the 'In Silico' analysis. 4. A new reference has been added on page 32. 5. Five lines have been deleted from 'In Silico analysis' in the methods section