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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Talaromyces sp. DC2 is an endophytic fungus that was isolated from the stem of Catharanthus roseus (L.) G. Don in Hanoi, Vietnam and is capable of producing vinca alkaloids. This study utilizes the PacBio Sequel technology to completely sequence the whole genome of Talaromyces sp. DC2The genome study revealed that DC2 contains a total of 34.58 Mb spanned by 156 contigs, with a GC content of 46.5%. The identification and prediction of functional protein-coding genes, tRNA, and rRNA were comprehensively predicted and highly annotated using various BLAST databases, including non-redundant (Nr) protein sequence, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Clusters of Orthologous Groups (COG), and Carbohydrate-Active Enzymes (CAZy) databases. The genome of DC2 has a total of 149, 227, 65, 153, 53, and 6 genes responsible for cellulose, hemicellulose, lignin, pectin, chitin, starch, and inulin degradation, respectively. The Antibiotics and Secondary Metabolites Analysis Shell (AntiSMASH) analyses revealed that strain DC2 possesses 20 biosynthetic gene clusters responsible for producing secondary metabolites. The strain DC2 has also been found to harbor the DDC gene encoding aromatic L-amino acid decarboxylase enzyme. Conclusively, this study has provided a comprehensive understanding of the processes involved in secondary metabolites and the ability of the Talaromyces sp. DC2 strain to degrade plant cell walls.

Details

Title
Genome Characteristics of the Endophytic Fungus Talaromyces sp. DC2 Isolated from Catharanthus roseus (L.) G. Don
Author
Nguyen, Duc Quan 1   VIAFID ORCID Logo  ; Ngoc-Lan, Nguyen 2 ; Tran Thi Huong Giang 1 ; Nguyen Thi Thanh Ngan 2 ; Nguyen Thanh Hien 1 ; Nguyen Van Tung 2   VIAFID ORCID Logo  ; Nguyen Hoang Thanh Trang 1 ; Nguyen Thi Kim Lien 2 ; Huy Hoang Nguyen 2   VIAFID ORCID Logo 

 Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, Vietnam; [email protected] (N.D.Q.); [email protected] (N.-L.N.); [email protected] (T.T.H.G.); [email protected] (N.T.T.N.); [email protected] (N.T.H.); [email protected] (N.V.T.); [email protected] (N.H.T.T.); [email protected] (N.T.K.L.) 
 Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, Vietnam; [email protected] (N.D.Q.); [email protected] (N.-L.N.); [email protected] (T.T.H.G.); [email protected] (N.T.T.N.); [email protected] (N.T.H.); [email protected] (N.V.T.); [email protected] (N.H.T.T.); [email protected] (N.T.K.L.); Department of Biotechnology, Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, Vietnam 
First page
352
Publication year
2024
Publication date
2024
Publisher
MDPI AG
e-ISSN
2309608X
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3059523723
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.