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Introduction: In both rats and humans, methamphetamine (Meth) seeking progressively increases during abstinence, a behavioral phenomenon termed “incubation of Meth craving”. We previously demonstrated a critical role of dorsal striatum (DS) in this incubation in rats. However, circuit-specific molecular mechanisms in DS underlying this incubation are largely unknown. Here we combined a newly developed fluorescence-activated sorting (FACS) protocol with fluorescence-conjugated cholera toxin subunit B-647 (CTb-647, a retrograde tracer) to examine gene alterations in the direct-pathway (striatonigral) medium spiny neurons (MSNs) associated with incubation of Meth craving.

Details

1009240
Subject
Behavior;
Cholera toxin;
Catheters;
Caudate-putamen;
Molecular modelling;
Spiny neurons;
Flow cytometry;
Genes;
Neostriatum;
Laboratory animals;
Narcotics;
Proteins;
Fluorescence;
Abstinence;
Polyethylene;
Dopamine;
Surgery;
FDA approval;
Experiments;
Methamphetamine;
Cholera;
Enzymes;
Toxins;
Roles;
Neurosciences
Business indexing term
Location
United States--US
Company / organization
Name:
University of Maryland
NAICS:
611310
Identifier / keyword
gene expression; methamphetamine craving; cholera toxin subunit B; fluorescence-activated cell sorting; striatonigral projection neurons
Title
Profiling gene alterations in striatonigral neurons associated with incubation of methamphetamine craving by cholera toxin subunit B-based fluorescence-activated cell sorting
Author
Altshuler, Rachel D; Burke, Megan A M; Garcia, Kristine T; Class, Kenneth; Cimbro, Raffaello; Li, Xuan
Publication title
Frontiers in Cellular Neuroscience; Lausanne
Publication year
2025
Publication date
Feb 12, 2025
Publisher
Frontiers Research Foundation
Place of publication
Lausanne
Country of publication
Switzerland
Publication subject
Medical Sciences--Psychiatry And Neurology
e-ISSN
16625102
Source type
Scholarly Journal
Language of publication
English
Document type
Journal Article
Publication history
 
 
Online publication date
2025-01-20
Milestone dates
2024-12-09 (Received); 2025-01-20 (Accepted); 2025-02-12 (Published)
Publication history
 
 
   First posting date
20 Jan 2025
DOI
https://doi.org/10.3389/fncel.2025.1542508
ProQuest document ID
3165948050
Document URL
https://www.proquest.com/scholarly-journals/profiling-gene-alterations-striatonigral-neurons/docview/3165948050/se-2?accountid=208611
Copyright
© 2025. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Last updated
2025-08-04
Database
ProQuest One Academic