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Abstract
Low-doses of irradiation have been reported to have beneficial effects, particularly anti-tumor effects. In this paper, we show the effects of the low-dose irradiation on T cell activation induced by dendritic cells (DCs). DCs, which had been pre-irradiated at 0.02-1.0 Gy from a 137Cs source, were cultured with allogeneic T cells, and the proliferation of T cells was then examined. The 0.05Gy-pre-irradiated DCs showed the highest proliferation capacity of T cells. The 0.05Gy-irradiation does not augment the expression of major histocompatibility complexes (MHCs) or costimulatory molecules on DCs, as with non-irradiated DCs or 1Gy-irradiated DCs, but does augment the production of IL-2, IL-12 and IFN-γ DCs. These results suggest that the low-dose irradiation augments T cell-activation capacity through cytokine production by DCs, which might shift naïve helper T cells to Th1 cells.
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1 First Department of Pathology, Kansai Medical University, 10-15 Fumizonocho, Moriguchi city, Osaka 570-8506, Japan; Department of Hematology and Oncology, Hokkaido University Graduate School of Medicine, Kita-15 Nishi-7, Kita-ku, Sapporo city, Hokkaido 060-8638, Japan
2 First Department of Pathology, Kansai Medical University, 10-15 Fumizonocho, Moriguchi city, Osaka 570-8506, Japan; Regeneration Research Center for Intractable Diseases, Kansai Medical University, 10-15 Fumizonocho, Moriguchi city, Osaka 570-8506, Japan; Center for Cancer Therapy, Kansai Medical University, 10-15 Fumizonocho, Moriguchi city, Osaka 570-8506, Japan
3 First Department of Pathology, Kansai Medical University, 10-15 Fumizonocho, Moriguchi city, Osaka 570-8506, Japan
4 Department of Hematology and Oncology, Hokkaido University Graduate School of Medicine, Kita-15 Nishi-7, Kita-ku, Sapporo city, Hokkaido 060-8638, Japan
5 First Department of Pathology, Kansai Medical University, 10-15 Fumizonocho, Moriguchi city, Osaka 570-8506, Japan; Department of Transplantation for Regeneration Therapy, Kansai Medical University, 10-15 Fumizonocho, Moriguchi city, Osaka 570-8506, Japan