Somatic Mutation in Larvae of the Silkworm, Bombyx mori, Induced by Heavy Ion Irradiation to Diapause Eggs

Abstract

In order to investigate whether eggs of the black-striped strain (P^S) of the silkworm, Bombyx mori, represent an appropriate model for estimating the biological effect of cosmic radiation, radiosensitivity of the eggs against X-rays and heavy ion particles was examined as ground-based experiments. The exposure of diapause eggs to X-rays or heavy ion particles resulted in somatic mutations appearing as a white spot on the black integument during larval stage. Irradiation of non-diapause eggs with X-rays demonstrated a significant difference in frequency of the mutation between fractionated and single administration doses, but no difference was observed in diapause eggs. Incidence of the mutation as induced by carbon ion beams for 15-day old eggs was higher for eggs that had been kept at 15°C than those kept at 25°C. Neon beam irradiation of diapause eggs displayed dose- and linear energy transfer (LET)-dependent effects, causing a maximal rate of the mutation at 150 keV/μm. These results confirm that B. mori eggs represent valid models for estimating the biological effects of cosmic radiation.