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Objective: The objective of this research was to determine the physicochemical composition of honey produced in the Sierra de Flores Magón region, Oaxaca, Mexico, to characterize its physicochemical parameters and verify Whether it meets the specifications established in national and international regulations. Theoretical Framework: Honey produced in Mexico has a varied composition and is well accepted in the international market. However, evidence is lacking to support the appropriate composition of honey, free of signs of adulteration. Method: A total of 29 honey samples from three municipalities in the Sierra de Flores Magón were collected directly from apiaries. The protocols of the Official Mexican Standard and the Codex Alimentarius were followed to determine: moisture, total soluble solids, electrical conductivity, ash, pH, acidity (free, lactone, and total), hydroxymethylfurfural (НМ), diastase index, and color. Results and Discussion: The honeys met both national and international regulations. A significant difference (P < 0.05) was found in conductivity and ash content. Some parameters indicated that the honeys were fresh, with no signs of adulteration or fermentation. Research Implications: Compliance with regulations could improve honey marketing conditions and generate better income for beekeepers. Originality/Value: This research contributes to the understanding of the functional biological properties of honeys from the aforementioned region, improving the marketing process.