Abstract

Currently, there are no available pharmacological treatments to reduce or stop the development of CAVD other than surgical or transcatheter heart valve replacement. [...]a comprehensive understanding of mechanisms that drive the phenotype of bone-forming osteoblasts of VICs is vital for exploring new therapies for this disease. [...]after echocardiographic parameters were assessed by transthoracic echocardiography using a 15∼70 MHZ array probe (MX500D) linked to a Vevo 3100 Imaging system (Fujifilm Visualsonics, Canada), all animals were euthanized. [...]band intensities were quantified using Fiji ImageJ software, with the relative density calculated as the ratio of the target gene to GAPDH. 2.5. [...]the samples were observed and photographed. 2.6. [...]excess stain was removed by washing the cells with deionized water. 2.7.

Details

Title
Thrombospondin-1 Silencing Ameliorates Osteoblastic Differentiation of Aortic Valve Interstitial Cells via Inhibiting Nuclear Factor-κB Pathway
Author
Li, Qing 1   VIAFID ORCID Logo  ; Song, Chengxiang 2   VIAFID ORCID Logo  ; Zisong Wei 3   VIAFID ORCID Logo  ; Li, Junli 3   VIAFID ORCID Logo  ; Zhou, Hao 3   VIAFID ORCID Logo  ; Wang, Shuoding 2   VIAFID ORCID Logo  ; Li, Hongde 2   VIAFID ORCID Logo  ; Yang, Haoran 2   VIAFID ORCID Logo  ; Luo, Qiang 2   VIAFID ORCID Logo  ; Chen, Mao 2   VIAFID ORCID Logo 

1  Laboratory of Cardiac Structure and Function Institute of Cardiovascular Diseases West China Hospital Sichuan University Chengdu China; Cardiac Structure and Function Research Key Laboratory of Sichuan Province West China Hospital Sichuan University Chengdu China; National Clinical Research Center for Geriatrics The Center of Gerontology and Geriatrics West China Hospital Sichuan University Chengdu China 
2  Laboratory of Cardiac Structure and Function Institute of Cardiovascular Diseases West China Hospital Sichuan University Chengdu China; Cardiac Structure and Function Research Key Laboratory of Sichuan Province West China Hospital Sichuan University Chengdu China; Department of Cardiology West China Hospital Sichuan University Chengdu China 
3  Laboratory of Cardiac Structure and Function Institute of Cardiovascular Diseases West China Hospital Sichuan University Chengdu China; Cardiac Structure and Function Research Key Laboratory of Sichuan Province West China Hospital Sichuan University Chengdu China 
Editor
Baohui Xu
Publication year
2025
Publication date
2025
Publisher
John Wiley & Sons, Inc.
ISSN
17555914
e-ISSN
17555922
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1155/cdr/3845211
ProQuest document ID
3191888214
Copyright
Copyright © 2025 Qing Li et al. Cardiovascular Therapeutics published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License (the “License”), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0/