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This paper presents a low-cost wireless prototype designed for point-of-care DNA sensing based on square wave voltammetry (SWV) measurements. Unlike other designs found in the literature, this prototype employs dedicated ADC and DAC components to reduce noise and allows for lower voltage steps in SWV scans. On-board signal processing makes the device suitable for use by inexperienced end-users. The prototype transmits data via Bluetooth Low-Energy (BLE) to a mobile app, which records the measurements on a cloud platform. The prototype was employed to detect a 23-base single-stranded DNA (ssDNA) sequence, within the range of 1 nM to 10 nM. The results obtained with the prototype showed good agreement when compared to a commercial electrochemical analyzer. This study demonstrates the feasibility of using such a device for DNA sensing, highlighting its potential for broader biosensing applications.
Details
Noise reduction;
Electrodes;
Applications programs;
Square waves;
Prototypes;
Mobile computing;
Biosensors;
MicroRNAs;
Measurement techniques;
Voltammetry;
Single-stranded DNA;
Feasibility studies;
Nucleotide sequence;
Point of care testing;
Digital to analog converters;
Signal processing;
Low cost;
Oxidation;
Signal to noise ratio;
Sensors;
Deoxyribonucleic acid--DNA;
Biomarkers
; Villarino Ramon 1
; Girbau, David 1
; Haji-Hashemi Hedieh 2
; Prieto-Simon, Beatriz 3
1 Department of Electronics, Electrics and Automatic Control Engineering, Rovira i Virgili University, 43007 Tarragona, Spain; [email protected] (R.V.); [email protected] (D.G.)
2 Institute of Chemical Research of Catalonia (ICIQ), Av. Paisos Catalans 16, 43007 Tarragona, Spain; [email protected] (H.H.-H.); [email protected] (B.P.-S.)
3 Institute of Chemical Research of Catalonia (ICIQ), Av. Paisos Catalans 16, 43007 Tarragona, Spain; [email protected] (H.H.-H.); [email protected] (B.P.-S.), Catalan Institution for Research and Advanced Studies (ICREA), Pg. Lluís Companys 23, 08010 Barcelona, Spain