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Abstract
The anthocyanin biosynthetic pathway is conserved across plant species and occurs via the phenylalanine metabolic pathway (Baudry et al. 2004). To investigate the function of MdZAT1, an analysis of MdZAT1 CDS firstly cloned from the fruit peel showed that the gene encoded a protein of 347 amino acids with a calculated molecular mass of 41.64 kDa. The anthocyanin content increased gradually with fruit development, while the expression of MdZAT1 decreased (Fig. 1H), suggesting that MdZAT1 may be a negative regulator in anthocyanin pathway. [...]according to the expression of related genes in transgenic materials, we tested whether MdZAT1 binds to the genes MdCHS, MdCHI, MdF3H, MdDFR, MdANS, MdUFGT, MdMYB1, MdGST, MdbHLH3 and MdbHLH33 promoters.