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Abstract
Multimodal bioimaging is a broad term used to describe experimental workflows that employ two or more different imaging modalities. Such approaches have been in use across life science domains for several years but these remain relatively limited in scope, in part due to the complexity of undertaking these types of analysis. Expanding these workflows to encompass diverse, emerging technology holds potential to revolutionize our understanding of spatial biology. In this perspective we reflect on the instrument and workflows in current use, emerging areas to consider and our experience of the barriers to broader adoption and progress. We propose several enabling solutions across the different challenge areas, emerging opportunities for consideration and highlight some of the key community activities to help move the field forward.
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1 Euro-BioImaging ERIC Bio-Hub, European Molecular Biology Laboratory (EMBL) Heidelberg, Heidelberg, Germany (GRID:grid.4709.a) (ISNI:0000 0004 0495 846X)
2 BioImagingUK and Royal Microscopical Society 37/38 St Clements Oxford, Oxford, UK (GRID:grid.4709.a); King’s College London, Randall Centre for Cell and Molecular Biophysics, London, UK (GRID:grid.13097.3c) (ISNI:0000 0001 2322 6764)
3 University of Bristol, School of Biochemistry, Bristol, UK (GRID:grid.5337.2) (ISNI:0000 0004 1936 7603)
4 Medical University of Vienna, Department of Biomedical Imaging and Image-guided Therapy, Vienna, Austria (GRID:grid.22937.3d) (ISNI:0000 0000 9259 8492)
5 University of Gothenburg, Centre for Cellular Imaging, Sahlgrenska Academy, Gothenburg, Sweden (GRID:grid.8761.8) (ISNI:0000 0000 9919 9582)
6 Italian National Research Council (CNR), Euro-BioImaging ERIC Med-Hub, Institute of Biostructures and Bioimaging (IBB), Torino, Italy (GRID:grid.429699.9) (ISNI:0000 0004 1790 0507)
7 Ben-Gurion University of the Negev, Department of Software and Information Systems Engineering, Beer-Sheva, Israel (GRID:grid.7489.2) (ISNI:0000 0004 1937 0511)
8 Aalen University, Center of Optical Technologies, Aalen, Germany (GRID:grid.440920.b) (ISNI:0000 0000 9720 0711)
9 Maastricht University, Maastricht Multimodal Molecular imaging institute, Department FHML, Maastricht, The Netherlands (GRID:grid.5012.6) (ISNI:0000 0001 0481 6099)
10 Maastricht University, Institutes CARIM, GROW, and MHeNS, Department of Genetics and Cell Biology, Maastricht, the Netherlands (GRID:grid.5012.6) (ISNI:0000 0001 0481 6099); RWTH Aachen University Clinic, Institute for Molecular Cardiovascular Research (IMCAR), Aachen, Germany (GRID:grid.1957.a) (ISNI:0000 0001 0728 696X)
11 King’s College London, Randall Centre for Cell and Molecular Biophysics, London, UK (GRID:grid.13097.3c) (ISNI:0000 0001 2322 6764)