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We aimed to determine the influence of donors’ vascular function on renal function in recipients and to evaluate the role of Orai1 calcium channels as a potential marker. A prospective collaborative multicenter study was designed. Blood, aorta (HA), mesenteric arteries (HMAs) and corpus cavernosum (HCC) specimens were obtained from organ donors at the kidney procurement procedure (n = 60). Evolution (up to 2 years) of renal function measured as serum creatinine (SCr) and glomerular filtration rate (GFR) was evaluated in respective recipients (n = 64). Vascular responses were determined in HA, HMA and HCC from donors. Tumor necrosis factor-α, asymmetric dimethyl arginine and Orai1 were measured in plasma. Orai1 protein expression was also evaluated in each donor’s aorta. Endothelium-dependent vasodilation (HMA, HCC) and adrenergic contraction (HA) in donors determined renal function in recipients, 12 months post-transplantation. Donors in the best quartile of vascular function predicted lower SCr and higher GFR in kidney recipients for 12/24 months. Plasma Orai1 in donors was negatively correlated with vascular function and predicted renal function at 3–6 months post-transplantation. Donor Orai1 vascular content was associated with reduced vascular function and with poorer recipient renal function for 1-year post-transplantation. Systemic vascular function of kidney donors determines recipients’ renal function at short/mid-term. Donors’ vascular function and recipients’ renal function are negatively associated with donors’ Orai1 vascular expression, being a potential biomarker of renal outcomes.
Details
Endothelium;
Antibodies;
Arteries;
Orai1 protein;
Aorta;
Coronary vessels;
Kidney diseases;
Kidney transplantation;
Renal function;
Biomarkers;
Tumor necrosis factor-TNF;
Vasodilation;
Kidney transplants;
Calcium channels;
Blood & organ donations;
Organ donors;
Brain death;
Pathophysiology;
Creatinine
; Angulo, Javier 2
; El Assar Mariam 3 ; Santos-Pérez de la Blanca Rocío 4 ; García-Gómez Borja 4 ; Medina-Polo, José 4
; Sevilleja-Ortiz Alejandro 5 ; Rodríguez-Mañas Leocadio 6
; Fernández Argentina 5 ; Gutiérrez-Martínez, Eduardo 7 ; Morales-Ruiz, Enrique 7 ; Rodríguez-Antolín, Alfredo 4 ; Romero-Otero, Javier 1 1 Department of Urology, Hospital Universitario HM Sanchinarro, HM Hospitales, 28050 Madrid, Spain; [email protected] (E.G.-R.); [email protected] (J.R.-O.)
2 Department of Histology-Research, Unidad de Investigación Traslacional en Cardiología (IRYCIS-UFV), Hospital Universitario Ramón y Cajal, 28034 Madrid, Spain; [email protected] (A.S.-O.); [email protected] (A.F.), Centro de Investigación Biomédica en Red sobre Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III, 28029 Madrid, Spain; [email protected] (M.E.A.); [email protected] (L.R.-M.)
3 Centro de Investigación Biomédica en Red sobre Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III, 28029 Madrid, Spain; [email protected] (M.E.A.); [email protected] (L.R.-M.), Instituto de Investigación IdiPaz, 28029 Madrid, Spain, Fundación para la Investigación Biomédica, Hospital Universitario de Getafe, 28905 Getafe, Spain
4 Department of Urology, Hospital Universitario 12 de Octubre, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), 28041 Madrid, Spain; [email protected] (R.S.-P.d.l.B.); [email protected] (B.G.-G.); [email protected] (J.M.-P.); [email protected] (A.R.-A.)
5 Department of Histology-Research, Unidad de Investigación Traslacional en Cardiología (IRYCIS-UFV), Hospital Universitario Ramón y Cajal, 28034 Madrid, Spain; [email protected] (A.S.-O.); [email protected] (A.F.)
6 Centro de Investigación Biomédica en Red sobre Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III, 28029 Madrid, Spain; [email protected] (M.E.A.); [email protected] (L.R.-M.), Instituto de Investigación IdiPaz, 28029 Madrid, Spain, Department of Geriatrics, Hospital Universitario de Getafe, 28905 Getafe, Spain
7 Department of Nephrology, Hospital Universitario 12 de Octubre, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), 28041 Madrid, Spain; [email protected] (E.G.-M.); [email protected] (E.M.-R.)