Content area
Abstract
ABSTRACT
Introduction
L‐Quebrachitol (LQB), a naturally occurring bioactive compound, exhibits anti‐inflammatory, anti‐oxidant, anti‐cancer, and anti‐diabetic properties. However, its therapeutic potential in convulsant management remains largely unexplored. The objective of this study was to investigate the anticonvulsant effects of LQB in an In Vivo model and to examine its molecular interactions via In Silico docking simulations.
Methods
In the In Vivo study, pentylenetetrazol (PTZ) was administered intraperitoneally (i.p.) at 80 mg/kg to induce convulsions, and the test animals were treated orally with three doses of LQB (1, 5, and 10 mg/kg), with carbamazepine (CBZ) at 80 mg/kg as a standard drug.
Results
The results indicated that LQB at all tested doses significantly (p < 0.05) prolonged seizure latency and decreased convulsion frequency, with the 10 mg/kg dose showing the most significant effects. Furthermore, the combination of LQB (10 mg/kg) and CBZ (80 mg/kg) resulted in a synergistic increase in anticonvulsant activity. In the In Silico study, molecular docking analysis revealed that both LQB and CBZ interacted with the voltage‐gated sodium channel (VGSC), a key receptor involved in convulsions, with LQB demonstrating a binding affinity (BA) of −5.4 kcal/mol, which was moderate compared to CBZ's BA.
Conclusion
LQB showed potential anti‐convulsant activity in PTZ‐induced convulsion animals, possibly through blocking sodium channel receptors. Further studies are needed to clarify its mechanisms and clinical potential in convulsion treatment.
Details
; Nun, Feroz Khan 3 ; Amin, Md. Faisal 5 ; Islam, Muhammad Torequl 6
1 Department of Biochemistry and Molecular Biology, Gopalganj Science and Technology University, Gopalganj, Bangladesh
2 Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia
3 Department of Pharmacy, Gopalganj Science and Technology University, Gopalganj, Bangladesh
4 Department of Pharmacy, Gopalganj Science and Technology University, Gopalganj, Bangladesh, Bioinformatics and Drug Innovation Laboratory, BioLuster Research Center Ltd., Gopalganj, Bangladesh
5 School of Integrative Biological and Chemical Sciences, The University of Texas Rio Grande Valley, Texas, USA
6 Department of Pharmacy, Gopalganj Science and Technology University, Gopalganj, Bangladesh, Bioinformatics and Drug Innovation Laboratory, BioLuster Research Center Ltd., Gopalganj, Bangladesh, Pharmacy Discipline, Khulna University, Khulna, Bangladesh