Content area
Moringa peregrina (Forssk.) Fiori is a tropical tree in southern Iran known as the most important natural coagulant in the world. Today, plant tissue culture is a new method that has a very high potential to produce valuable medicinal compounds on a commercial level. Advances in in vitro cultivation methods have increased the usefulness of plants as renewable resources. In this study, in addition to the phytochemical analysis of the extract of M. peregrina using HPLC, the interaction effect of different concentrations of aqueous extract of M. peregrina (0, 1, 1.5, and 3 mg/L) in two types of MS and ½ MS basal culture media over three weeks on the in vitro growth of Staurogyne repens (Nees) Kuntze was studied. The amounts of quercetin, gallic acid, caffeic acid, and myricetin in the aqueous extract of M. peregrina were 64.9, 374.8, 42, and 4.6 mg/g, respectively. The results showed that using M. peregrina leaf aqueous extract had a positive effect on the length of the branches, the percentage of green leaves, rooting, and the fresh and dry weight of S. repens samples. The highest increase in growth indices was observed in the MS culture medium supplemented with 3 mg/L of M. peregrina leaf aqueous extract after three weeks of cultivation. Of course, this effect was significantly greater in the MS medium and at higher concentrations compared to the ½ MS medium. Three weeks after cultivation at a concentration of 3 mg/L of the extract, the length of the S. repens branches was 5.3 and 1.8 cm in the two basic MS and ½ MS culture media, and the percentage of green leaves was 14 and 4 percent, respectively. Also, rooting was measured at 9.6 and 3.6 percent, fresh weight at 6 and 1.4 g, and dry weight at 1.1 and 0.03 g, respectively. Therefore, adding M. peregrina leaf aqueous extract as a stimulant significantly increased the in vitro growth of S. repens.
Details
In vitro methods and tests;
Acids;
Investigations;
Flowers & plants;
Plant tissues;
Phytochemicals;
Leaves;
Sustainable yield;
Culture media;
Renewable resources;
Coagulants;
Gallic acid;
Caffeic acid;
Branches;
Rooting;
Seeds;
Cultivation;
Flavonoids;
Antimicrobial agents;
Dry weight;
Quercetin;
Metabolites;
Tissue culture;
Moringa peregrina
; Salehi, Sardoei Ali 3 ; Fotoohiyan Zeinab 4 ; Hatami Mehrnaz 5
; Mirzaei Alireza 6 ; Mansour, Ghorbanpour 5
; Maggi Filippo 7
1 Agriculture Biotechnology Research Institute, University of Zabol, Zabol 98615538, Iran; [email protected]
2 Department of Agronomy and Plant Breeding, Agriculture Institute, Research Institute of Zabol, Zabol 9861335884, Iran; [email protected]
3 Crop and Horticultural Science Research Department, South Kerman Agricultural and Natural Resources Research and Education Center, AREEO, Jiroft 11578615, Iran
4 Department of Plant Pathology, Jiroft Branch, Islamic Azad University, Jiroft 7861736343, Iran; [email protected]
5 Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University, Arak 3815688349, Iran; [email protected]
6 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil 5619911367, Iran; [email protected]
7 Chemistry Interdisciplinary Project (ChIP) Research Center, School of Pharmacy, University of Camerino, Via Madonna delle Carceri, 62032 Camerino, Italy; [email protected]