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Abstract

The giant squid (Dosidicus gigas) is an abundant marine species with high protein content, making it a promising resource for the food and biomaterial industries. This study aimed to investigate the effect of temperature (25–100 °C) on the structural changes in sarcoplasmic, myofibrillar, and stromal proteins isolated from squid mantle. Fourier-transform infrared spectroscopy (FT-IR) and circular dichroism (CD) were employed to monitor modifications in secondary structure, while field emission scanning electron microscopy (FE-SEM) was used to examine morphological characteristics. The FT-IR analysis revealed temperature-induced transitions in amide I, II, and A bands, indicating unfolding and aggregation processes, particularly in myofibrillar and stromal proteins. CD results confirmed a loss of α-helix content and an increase in β-sheet structures with rising temperature, especially above 60 °C, suggesting progressive denaturation. FE-SEM micrographs illustrated clear morphological differences: sarcoplasmic proteins displayed smooth, amorphous structures; myofibrillar proteins exhibited fibrous, porous networks; and stromal proteins presented dense and layered morphologies. These findings highlight the different thermal sensitivities and structural behaviors of squid muscle proteins and provide insight into their potential functional applications in thermally processed foods and bio-based materials.

Details

1009240
Taxonomic term
Title
Temperature-Induced Structural Changes in Muscle Proteins from Giant Squid (Dosidicus gigas) Mantle: FT-IR, Circular Dichroism, and FE-SEM Analysis
Author
León-Heredia, Miguel A 1 ; Marquez-Rios, Enrique 2 ; Cadena-Cadena, Francisco 3   VIAFID ORCID Logo  ; Santacruz-Ortega Hisila 4 ; Rivero-Espejel Ignacio Alfredo 5   VIAFID ORCID Logo  ; Montoya-Camacho Nathaly 6   VIAFID ORCID Logo  ; Tolano-Villaverde, Iván J 1 

 Department of Food Technology Engineering, Universidad Estatal de Sonora, Campus Hermosillo, Ley Federal del Trabajo s/n, Hermosillo 83100, SON, Mexico; [email protected] 
 Department of Food Research and Graduate Studies, Universidad de Sonora, Boulevard Luis Encinas y Rosales, Hermosillo 83000, SON, Mexico 
 National Technological Institute from Mexico, Valle del Yaqui Campus, Road 600, San Ignacio Río Muerto 85276, SON, Mexico; [email protected] 
 Department of Research in Polymers and Materials, Universidad de Sonora, Boulevard Luis Encinas y Rosales, Hermosillo 83000, Son, Mexico; [email protected] 
 Graduate and Research Center in Chemistry, Instituto Tecnológico de Tijuana, Tijuana 22414, BC, Mexico; [email protected] 
 Department of Chemical Biological Sciences, Universidad de Sonora, Boulevard Luis Encinas y Rosales, Hermosillo 83000, SON, Mexico; [email protected] 
Publication title
Foods; Basel
Volume
14
Issue
17
First page
2922
Number of pages
14
Publication year
2025
Publication date
2025
Publisher
MDPI AG
Place of publication
Basel
Country of publication
Switzerland
Publication subject
e-ISSN
23048158
Source type
Scholarly Journal
Language of publication
English
Document type
Journal Article
Publication history
 
 
Online publication date
2025-08-22
Milestone dates
2025-06-30 (Received); 2025-08-15 (Accepted)
Publication history
 
 
   First posting date
22 Aug 2025
ProQuest document ID
3249680621
Document URL
https://www.proquest.com/scholarly-journals/temperature-induced-structural-changes-muscle/docview/3249680621/se-2?accountid=208611
Copyright
© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Last updated
2025-09-12
Database
ProQuest One Academic