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Abstract

Polyhydroxyalkanoate (PHA) bioplastics are produced from wastewater as a carbon recovery strategy. However, the tuneable characteristics of PHAs and wastewater biorefinery potential have not been comprehensively reviewed. The aim of this study is to review the main challenges and strategies for carbon recovery from wastewater feedstocks via PHA production, assessing potential target biopolymer applications. Diverse PHA-accumulating prokaryotes metabolize organic pollutants present in wastewater through different metabolic pathways, determining the biopolymer characteristics. The synthesis of PHAs using mixed microbial cultures with wastewater feedstocks derived from municipal, agro-industrial, food processing, lignocellulosic biomass processing and biofuel production activities are described. Acidogenic fermentation of wastewater feedstocks and mixed microbial culture enrichment are key steps in order to enhance PHA productivity and determine biopolymer properties towards customized bioplastics for specific applications. Biorefinery of PHA copolymers and extracellular polysaccharides (EPSs), including alginate-like polysaccharides, are alternatives to enhance the value-chain of carbon recovery from wastewater. PHAs and EPSs exhibit a wide repertoire of applications with distinct safety control requirements; hence, coupling biopolymer production demonstrations with target applications is crucial to move towards full-scale applications. This study discusses the relationship between the metabolic basis of PHA synthesis and composition, wastewater type, and target applications, describing the potential to maximize carbon resource valorisation.

Details

1009240
Title
Carbon Recovery from Wastewater Feedstocks: Synthesis of Polyhydroxyalkanoates for Target Applications
Author
Sepúlveda, Mario I 1 ; Seeger, Michael 1   VIAFID ORCID Logo  ; Vidal, Gladys 2   VIAFID ORCID Logo 

 Molecular Microbiology and Environmental Biotechnology Laboratory, Department of Chemistry, Center of Biotechnology Daniel Alkalay Lowitt, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso 2390123, Chile; [email protected] (M.I.S.); [email protected] (M.S.), Millenium Nucleus Bioproducts, Genomics and Environmental Microbiology (BioGEM), Avenida España 1680, Valparaíso 2390123, Chile 
 Environmental Engineering & Biotechnology Group (GIBA-UDEC), Environmental Science Faculty, Universidad de Concepción, Concepción 4070386, Chile, Water Research Center for Agriculture and Mining (CRHIAM), ANID Fondap Center, Victoria 1295, Concepción 4070411, Chile 
Publication title
Resources; Basel
Volume
14
Issue
10
First page
156
Number of pages
22
Publication year
2025
Publication date
2025
Publisher
MDPI AG
Place of publication
Basel
Country of publication
Switzerland
Publication subject
e-ISSN
20799276
Source type
Scholarly Journal
Language of publication
English
Document type
Journal Article
Publication history
 
 
Online publication date
2025-10-01
Milestone dates
2025-08-02 (Received); 2025-09-25 (Accepted)
Publication history
 
 
   First posting date
01 Oct 2025
ProQuest document ID
3265941005
Document URL
https://www.proquest.com/scholarly-journals/carbon-recovery-wastewater-feedstocks-synthesis/docview/3265941005/se-2?accountid=208611
Copyright
© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Last updated
2025-11-03
Database
ProQuest One Academic