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Abstract

ABSTRACT

The present study examined the impacts of Lactococcus lactis D1813, salinity (8 and 25 ppt), and dissolved oxygen (DO) concentrations of 8.5 and 3.5 mg/L on Litopenaeus vannamei muscle and head lipidomic profile. The muscle and head were examined using liquid chromatography‐mass spectrometry (LC–MS/MS) for lipidomic nutrient profiling. The lipidomic profiling revealed the primary nutritional metabolites in L. vannamei muscle and head, which are composed of glycerophospholipids, sphingolipids, sterols, and saturated fatty acids. Huang head had the most glycerophospholipids and sterols, including PC (12:0/18:2), PC (18:0/20:4), PC (16:1/22:6), PC (10:1e/20:4), PC (33:0/18:2), Cer (d14:1/20:0), SM (t18:1/22:4), and SM (d18:0/16:1). Conversely, the T3BS head group had a much greater proportion of sphingolipids and saturated fatty acids, including Cer (d14:1/20:0), SM (t18:1/22:4), SM (d18:0/16:1), SM (d18:1/18:3), and butyric acid, stearic acid, myristic acid, arachidic acid, and lauric acid (p < 0.05). The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated the significant enrichment of glycerophospholipid metabolism, sphingolipid metabolism, glycerolipid metabolism, linolenic acid metabolism, and alpha‐linolenic acid metabolism in T3BS muscle and head. The findings unequivocally demonstrate that the supplement of L. lactis D1813 under 25 ppt salinity and 3.5 mg/L DO levels significantly raises the content of sphingolipids and saturated fatty acids and enriches the metabolic pathways of linolenic acid and alpha‐linolenic acid in L. vannamei. The results indicated that L. lactis D1813 could be used as an effective probiotic to enhance shrimp tolerance and nutritional quality in aquaculture systems under environmental stress conditions. Also, a more desirable lipid profile renders them beneficial to human health, particularly cardiovascular health and brain function. Additional research can be done to assess the therapeutic advantages of L. vannamei‐derived lipids in humans regarding cardiovascular health, cancer, and brain functioning.

Details

1009240
Subject
Mass spectrometry;
Lauric acid;
Liquid chromatography;
Butyric acid;
Probiotics;
Muscles;
Salinity effects;
Stearic acid;
Dissolved oxygen;
Sterols;
Brain;
Metabolites;
Metabolism;
Oxidative stress;
Water quality;
Lipids;
Linolenic acid;
Fatty acids;
Metabolic pathways;
Lipid metabolism;
Mass spectroscopy;
Environmental stress;
Aquaculture;
Sphingolipids;
Salinity;
Reagents;
Polyunsaturated fatty acids;
Environmental conditions;
Caustic soda;
Signal transduction;
Nutritive value;
Hydrochloric acid;
Phospholipids;
Litopenaeus vannamei;
Lactococcus lactis
Location
United States--US; China
Taxonomic term
Litopenaeus vannamei, Lactococcus lactis
Identifier / keyword
aquaculture; KEGG; lipid metabolism; metabolites; probiotics; shrimps
Title
Lipidomic Characterization of Muscle and Head of Litopenaeus vannamei Exposed to Lactococcus lactis D1813 at Varied Levels of Salinity and Dissolved Oxygen
Author
Adil, Muhammad 1 ; Xiao, Xinglong 1   VIAFID ORCID Logo  ; Waseem, Muhammad 2 ; Afraz, Muhammad Talha 1 ; Javed, Muhammad Rizwan 2 ; Alohali, Basim M. 3 ; Mohamed Ahmed, Isam A. 3 ; Manzoor, Muhammad Faisal 4 ; Madilo, Felix Kwashie 5   VIAFID ORCID Logo 

1  School of Food Science and Engineering, South China University of Technology, Guangzhou, China 
2  Department of Food Science & Technology, Faculty of Agriculture & Environment, The Islamia University of Bahawalpur, Punjab, Pakistan 
3  Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia 
4  School of Food Science and Engineering, South China University of Technology, Guangzhou, China, Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, School of Food Science and Engineering, Foshan University, Foshan, China, Faculty of Sciences and Technology, ILMA University, Karachi, Pakistan 
5  Food Science and Technology Department, Ho Technical University, Ho, Ghana 
Publication title
Food Science & Nutrition; Malden, Massachusetts
Volume
13
Issue
11
Number of pages
16
Publication year
2025
Publication date
Nov 1, 2025
Section
ORIGINAL ARTICLE
Publisher
John Wiley & Sons, Inc.
Place of publication
Malden, Massachusetts
Country of publication
United States
Publication subject
Nutrition And Dietetics
e-ISSN
20487177
Source type
Scholarly Journal
Language of publication
English
Document type
Journal Article
Publication history
 
 
Online publication date
2025-10-29
Milestone dates
2025-09-30 (manuscriptRevised); 2025-10-29 (publishedOnlineFinalForm); 2025-05-15 (manuscriptReceived); 2025-10-10 (manuscriptAccepted)
Publication history
 
 
   First posting date
29 Oct 2025
DOI
https://doi.org/10.1002/fsn3.71141
ProQuest document ID
3266605420
Document URL
https://www.proquest.com/scholarly-journals/lipidomic-characterization-muscle-head/docview/3266605420/se-2?accountid=208611
Copyright
© 2025. This work is published under http://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Last updated
2025-12-06
Database
ProQuest One Academic