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In nursing, the introduction of evidence-based practice (EBP) was intended to bridge the divide between theory-driven research and nursing practice, allowing theory-driven nursing research to inform nursing practice. Pre-licensure nursing education and healthcare organizations have engaged in considerable effort around the introduction of EBP and subsequent promotion and educational initiatives. However, the continued low use of EBP by nursing professionals in the clinical setting indicates that barriers to its use remain. The purpose of this study is to determine whether nursing confidence is a barrier to the use of EBP through additional validation of the Nurse Confidence in Evidence-Based Practice (RNcEBP) survey instrument. Bandura’s theory of self-efficacy and Nightingale’s environmental theory were used for the theoretical framework, providing contextual understanding of nursing confidence and its relationship to EBP and EBP use. The research question was as follows: What is the relationship between nurse confidence and evidence-based practice utilization? The study employed a mixed-methods design by using the RNcEBP survey and Evidence-Based Practice Perception Questions, four open-ended questions expanding upon participants’ views of EBP in nursing. Data collected from the RNcEBP survey was analyzed using descriptive statistics, and open-ended question responses were assessed using Colaizzi’s descriptive phenomenological method. The results of the study showed that a relationship between nursing confidence and nursing utilization of EBP does exist. These findings highlight the need for education initiatives that focus on increasing nursing professionals’ individual confidence and efficacy in the utilization of EBP in their professional practice.