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The characterization of the chromosomal morphology of Haplopappus foliosus (Hook. & Arn.) determined a chromosomal number of 2n = 10, with a karyotypic formula of one subtelocentric pair (st) and four submetacentric pairs (sm). The intrachromosomal asymmetry index (A1) and interchromosomal asymmetry index (A2) showed a moderate tendency towards telocentricity and low variability in the sizes of chromosome pairs.