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Objectives: This study aimed to evaluate the cytotoxic effects of Ti6Al4V particles and implantoplasty (IP)-treated surfaces on human fibroblasts and osteoblasts, and to investigate the influence of these particles on multispecies oral biofilm formation. Methods: Ti6Al4V particles generated during implantoplasty were collected. Human fibroblasts (HFF-1) and osteoblast-like cells (SaOs-2) were used to assess cytotoxicity through indirect lactate dehydrogenase (LDH) assays. Multispecies biofilms composed of Streptococcus oralis, Actinomyces viscosus, Veillonella parvula and Porphyromonas gingivalis were evaluated based on colony-forming units (CFUs) and metabolic activity. Fibroblasts and osteoblasts were co-cultured with biofilm-contaminated particles for 2, 4 and 6 h. Cell morphology and biofilm association were examined by phase-contrast microscopy, while metabolic activity was measured spectrophotometrically. Results: IP-treated surfaces showed no significant cytotoxicity (metabolic activity > 92%, LDH < 20%). Ti6Al4V particles selectively promoted A. viscosus and V. parvula growth (metabolic activity increases of ≈192% and ≈203%; CFU significantly higher versus controls, p < 0.05). Co-culture with biofilm-contaminated particles drastically reduced cell activity (fibroblasts < 25%, osteoblasts < 10%), whereas bacteria-free particles did not. Conclusions: Biofilm-contaminated particles released during implantoplasty markedly impair fibroblast and osteoblast cytocompatibility and selectively alter bacterial growth, whereas IP-treated surfaces per se are biocompatible. Minimizing particle dissemination and bacterial contamination during IP is therefore crucial.
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; Figueiredo Rui 1
; Demiquels-Punzano Elena 2 ; Gil, Javier 3
; Delgado, Luis M 4
; Sanmartí-García Gemma 1 ; Camps-Font Octavi 1
1 School of Medicine and Health Sciences, Campus de Bellvitge, Universitat de Barcelona, C/Feixa Llarga s/n, Pavelló Govern 2ª Planta, Despatx 2.9, 08907 L’Hospitalet de Llobregat, Spain; [email protected] (E.V.-B.); [email protected] (M.Á.S.-G.); [email protected] (G.S.-G.); [email protected] (O.C.-F.), Dental and Maxillofacial Pathology and Therapeutics Research Group, IDIBELL Research Institute, 08908 Barcelona, Spain
2 Bioengineering Institute of Technology (BIT), Universitat International de Catalunya, 08017 Barcelona, Spain; [email protected]
3 Bionspired Oral Biomaterials Interfaces, Departament de Ciencia e Ingenieria de Materiales, Escola d’Engingeria Barcelona Est, Universitat Politécnica de Catalunya, Av. Eduard Maristany 16, 08019 Barcelona, Spain
4 Bionspired Oral Biomaterials Interfaces, Departament de Ciencia e Ingenieria de Materiales, Escola d’Engingeria Barcelona Est, Universitat Politécnica de Catalunya, Av. Eduard Maristany 16, 08019 Barcelona, Spain, Department of Graphic and Design Engineering, Universitat Politècnica de Catalunya (UPC), 08222 Terrasa, Spain