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Background/Aim: Suicide is a complex and multidimensional phenomenon that involves multiple variables, among which attitudes stand out. Given its usefulness for suicide prevention and the heteronomy found in the assessment of these attitudes, the aim of this study is to provide an updated synthesis of the instruments available for their evaluation. Method: A systematic review was conducted following PRISMA guidelines. The search included articles published up to December 2023 in the databases Scopus, PubMed, Web of Science, ProQuest, Cochrane, and the meta-search engine EBSCOHOST. Results: A total of 105,151 papers were obtained, of which 316 were subjected to a full-text screening that identified 41 studies that met the inclusion criteria. We found 14 original instruments, 11 validations in other samples, 13 psychometric studies and 3 papers that developed modified versions of previous questionnaires. Conclusions: This review can serve as a guide for the choice of tools for the assessment of attitudes towards suicide and, in addition, as a complement for studies on the prevention of suicidal behavior. In this way, the results facilitate responsible decision making based on the available evidence and adjusted to the needs of the professional.