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Abstract
High amounts of outer membrane (OM) components were released in glucose-limited fed-batch (GLFB) cultures at 37 °C at specific growth rates approaching 0.05 h^sup -1^. Endotoxin analyses from a 20 °C GLFB culture gave similar results. An alternative fermentation technique, the temperature-limited fed-batch (TLFB) technique, reduced the endotoxin concentration in a culture with a biomass concentration of 30 g l^sup -1^ from the 850 mg l^sup -1^ in traditional GLFB cultures to about 20 mg l^sup -1^. The TLFB technique uses the temperature to regulate the dissolved oxygen tension, while all substrate components are unregulated. It appears to be severe glucose limitation that triggers the extensive release of endotoxins rather than a low growth rate. Furthermore, it is not the low temperature that stabilizes the OM when using the TLFB technique. Simulations and experimental data show that this technique results in the same biomass productivity as the GLFB technique.[PUBLICATION ABSTRACT]