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Abstract

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Vector DNA (pBmFRT) microinjected into the silkworm eggs (preblastoderm stage) persisted in different conformational forms throughout the period of embryonic development. Southern blot analysis confirmed the persistence of DNA as extrachromosomal copies. Slot blot analysis showed the inheritance of the injected DNA to the subsequent progenies; however the copy number of the injected vector declined in the progenies.[PUBLICATION ABSTRACT]

Details

Subject
Deoxyribonucleic acid--DNA;
Embryonic growth stage
Identifier / keyword
Bombyx mori; Microinjection
Title
Extrachromosomal transmission of microinjected DNA to the progeny of silkworm Bombyx mori
Author
Shamila, Y; Mathavan, S
Publication title
Biotechnology Letters; Dordrecht
Volume
21
Issue
5
Pages
425-430
Publication year
1999
Publication date
May 1999
Publisher
Springer Nature B.V.
Place of publication
Dordrecht
Country of publication
Netherlands
Publication subject
Biology--Biotechnology, Biology--Microbiology
ISSN
0141-5492
e-ISSN
1573-6776
Source type
Scholarly Journal
Language of publication
English
Document type
Feature
DOI
https://doi.org/10.1023/A:1005537027739
ProQuest document ID
735205055
Document URL
https://www.proquest.com/scholarly-journals/extrachromosomal-transmission-microinjected-dna/docview/735205055/se-2?accountid=208611
Copyright
Kluwer Academic Publishers 1999
Last updated
2024-10-04
Database
ProQuest One Academic