Streptomyces gulbargensis sp. nov., isolated from soil in Karnataka, India

Abstract

During the course of screening for industrially important microorganisms, an alkalitolerant and thermotolerant actinomycete, strain DAS 131^sup T^, was isolated from a soil sample collected from the Gulbarga region, Karnataka province, India. The strain was characterized by a polyphasic approach that showed that it belonged to the genus Streptomyces. Growth was observed over a wide pH range (pH 6-12) and at 45°C. The 16S rRNA gene sequence of strain DAS 131^sup T^ was deposited in the GenBank database under the accession number DQ317411. 16S rRNA gene sequence analysis revealed that strain DAS 131^sup T^was most closely related to Streptomyces venezuelae ISP 5230^sup T^ (AY999739) with a sequence similarity of 99.5% (8 nucleotide differences out of 1,477). Despite this very high sequence similarity, strain DAS 131^sup T^ was phenetically distinct from S. venezuelae. The DNA relatedness between these strains was 54%, indicating that strain DAS 131^sup T^ is a distinct genomic species. On the basis of phenetic and genetic analyses, strain DAS 131^sup T^ is classified as a new species in the genus Streptomyces, for which we propose the name Streptomyces gulbargensis sp. nov.[PUBLICATION ABSTRACT]

During the course of screening for industrially important microorganisms, an alkalitolerant and thermotolerant actinomycete, strain DAS 131^sup T^, was isolated from a soil sample collected from the Gulbarga region, Karnataka province, India. The strain was characterized by a polyphasic approach that showed that it belonged to the genus Streptomyces. Growth was observed over a wide pH range (pH 6-12) and at 45°C. The 16S rRNA gene sequence of strain DAS 131^sup T^ was deposited in the GenBank database under the accession number DQ317411. 16S rRNA gene sequence analysis revealed that strain DAS 131^sup T^was most closely related to Streptomyces venezuelae ISP 5230^sup T^ (AY999739) with a sequence similarity of 99.5% (8 nucleotide differences out of 1,477). Despite this very high sequence similarity, strain DAS 131^sup T^ was phenetically distinct from S. venezuelae. The DNA relatedness between these strains was 54%, indicating that strain DAS 131^sup T^ is a distinct genomic species. On the basis of phenetic and genetic analyses, strain DAS 131^sup T^ is classified as a new species in the genus Streptomyces, for which we propose the name Streptomyces gulbargensis sp. nov.[PUBLICATION ABSTRACT]