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Biol Trace Elem Res (2010) 138:173180
DOI 10.1007/s12011-009-8599-z
Caimin Bai & Tao Chen & Yun Cui & Tao Gong & Xi Peng & Heng-min Cui
Received: 23 September 2009 /Accepted: 17 December 2009 /
Published online: 20 January 2010# Springer Science+Business Media, LLC 2010
Abstract The experiment was conducted with the objective of evaluating the effect of dietary high fluorine (F) on cell cycle and apoptosis of kidney in chickens by the methods of flow cytometry. Three hundred 1-day-old Avian broilers were divided into four groups and fed on control diet (F 23 mg/kg) and high F diets (400 mg/kg, high F group I; 800 mg/kg, high F group II; 1,200 mg/kg, high F group III) for 6 weeks. As tested by flow cytometry, the percentage of renal cell apoptosis was increased with increasing of dietary F, and it obviously rose in three high F groups when compared with that of control group. Renal cells in G0/G1 phase were much higher, and renal cells in S phase, G2+M phase, and proliferation index value were much lower in high F groups I, II, and III than in control group. The results showed that excess dietary F in the range of 4001,200 mg/kg caused G0/G1 arrest and increased cellular apoptosis in the kidney, which might finally interfere with the excretion and retention of fluoride in chickens.
Keywords Apoptosis . Cell cycle . Chicken . Flow cytometry . High fluorine . Renal cells
Abbreviations
PI Propidium iodidePI value Proliferation index value
Introduction
Fluorion at millimolar concentrations is known to influence many cellular functions in vitro [1]. At these concentrations, it has been shown to cause cytotoxic effects on human collecting and proximal ductal cells [1, 2]. Fluoride is a cumulative poison. On an average, only 50% of the fluoride ingested by our body each day is excreted through the kidneys while the remaining fluoride accumulates in our bones, pineal gland, and other tissues [3]. Kidney is well known as the primary organ involved in excretion and retention of fluoride and is a sensitive organ that shows a histopathological and functional response to excessive amount of fluoride [4]. As
Caimin Bai and Tao Chen contributed equally to this work.
C. Bai : T. Chen : Y. Cui : T....