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Int Urol Nephrol (2010) 42:401408 DOI 10.1007/s11255-009-9610-5
UROLOGY - ORIGINAL PAPER
Pygeum africanum: effect on oxidative stress in early diabetes-induced bladder
Dan Wang Yongzhi Li Guihua Hou Ping Wang Jianping Zhang Vincent Laudon Benkang Shi
Received: 13 May 2009 / Accepted: 23 June 2009 / Published online: 16 July 2009 Springer Science+Business Media, B.V. 2009
AbstractObjective To evaluate the effect of Pygeum africa-num on oxidative stress and functional changes of the bladder after diabetes induction.
Materials and methods Thirty-two adult Wistar male rats were treated daily for 8 weeks and grouped as follows: Control group (n = 6), Streptozotocin-induced diabetic group (n = 10), diabetes plusP. africanum group (n = 10), and control plusP. africanum group (n = 6). After diabetes induction
D. Wang G. Hou
Institute of Experimental Nuclear Medicine, Shandong University School of Medicine, 44# Wenhua Xi Road, 250012 Jinan, Shandong, China
D. Wange-mail: [email protected]
Y. Li P. Wang
Department of Urology, The Fourth Hospital of China Medical University, Shenyang, China
J. ZhangDepartment of Pathology, Qilu Hospital of Shandong University, Jinan, China
V. LaudonDivision of Urology, Hartford Hospital, Hartford, CT, USA
B. Shi (&)
Department of Urology, Qilu Hospital of Shandong University, 107# Wenhua Xi Road, 250012 Jinan, Shandong, Chinae-mail: [email protected]
for 4 weeks, the diabetes plus P. africanum and control plus P. africanum groups were fed withP. africanum (100 mg/kg, orally) in peanut oil for another 4 weeks. The catalase, superoxide dismutase activity, and malondialdehyde levels were measured as a marker of lipid peroxidation. The levels of inducible nitric oxide synthase were also evaluated. Urodynamic studies were performed to evaluate the functional changes of diabetic bladders afterP. africanum treatment.
Results The catalase and superoxide dismutase activities signicantly increased (P \ 0.05) and maleic dialdehyde levels signicantly decreased from diabetic plus P. africanum group compared with diabetic group (P \ 0.05). Immunohistochemical studies showed a signicantly decreased number of inducible nitric oxide synthase-positive cells in diabetic plus P. africanum group compared with diabetic group (P \ 0.05). In diabetic plus P. africa-num group, maximal bladder volume signicantly decreased, while bladder pressure and maximal bladder pressure signicantly increased compared with diabetic group (P \ 0.05).
Conclusions Early treatment with P. africanum could effectively suppress the oxidative stress status in diabetic bladder and may slow...