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Plant Cell Rep (2011) 30:177193 DOI 10.1007/s00299-010-0954-1
REVIEW
Links between lipid homeostasis, organelle morphodynamicsand protein trafcking in eukaryotic and plant secretory pathways
Su Melser Diana Molino Brigitte Batailler Martine Peypelut Maryse Laloi
Valrie Wattelet-Boyer Yannick Bellec Jean-Denis Faure Patrick Moreau
Received: 3 November 2010 / Accepted: 15 November 2010 / Published online: 1 December 2010 Springer-Verlag 2010
Abstract The role of lipids as molecular actors of protein transport and organelle morphology in plant cells has progressed over the last years through pharmacological and genetic investigations. The manuscript is reviewing the roles of various lipid families in membrane dynamics and trafcking in eukaryotic cells, and summarizes some of the related physicochemical properties of the lipids involved. The article also focuses on the specic requirements of the sphingolipid glucosylceramide (GlcCer) in Golgi morphology and protein transport through the plant secretory pathway. The use of a specic inhibitor of plant glucosylceramide synthase and selected Arabidopsis thaliana RNAi lines stably expressing several markers of the plant secretory pathway, establishes specic steps sensitive to GlcCer biosynthesis. Collectively, data of the literature demonstrate the existence of links between protein
trafcking, organelle morphology, and lipid metabolism/ homeostasis in eukaryotic cells including plant cells.
Keywords Secretory pathways Membrane dynamics
Protein trafcking Lipid homeostasis Lipid domains
Lipid machineries
Introduction to membrane dynamics: from a protein to a lipid world
Several protein machineries have been shown to be fundamental in the functional dynamic membrane organization of the plant secretory pathway (Moreau et al. 2007; Hanton et al. 2007, 2009; Woollard and Moore 2008; Richter et al. 2009; Sparkes et al. 2009; Grifng 2009; Hwang and Robinson 2009; Hawes et al. 2010). For example, the dynamic ER network (Sparkes et al. 2009; Grifng 2009), the structural features of the ER-Golgi interface and the ER
Communicated by R. Reski.
S. Melser V. Wattelet-Boyer P. Moreau (&)
Laboratoire de Biogense Membranaire, UMR 5200 Universit Bordeaux 2-CNRS, Universit Bordeaux 2, case 92,146 rue Lo-Saignat, 33076 Bordeaux, Francee-mail: [email protected]
S. Melsere-mail: [email protected]
V. Wattelet-Boyere-mail: [email protected]
D. Molino Y. Bellec J.-D. Faure
Laboratoire de Biologie Cellulaire, INRA UR501, Versailles, Francee-mail: [email protected]
Y. Bellece-mail: [email protected]
J.-D. Fauree-mail: [email protected]
B. Batailler M. Peypelut P. Moreau
Ple Imagerie du Vgtal du BIC (Bordeaux Imaging Center), INRA IFR103, Villenave dOrnon, Francee-mail: [email protected]
M. Peypelute-mail: [email protected]