Content area
Abstract
A Gram-negative, yellow pigmented bacterium designated strain CA5^sup T^ that reduced selenite to elemental red selenium was isolated from soil. 16S rRNA gene sequence alignment identified the isolate as a novel Pseudomonas species with P. argentinensis, P. flavescens, and P. straminea as its closest relatives. Sequence alignments show that the 16S rRNA gene of strain CA5^sup T^ differed from that of P. argentinensis, P. flavescens, and P. straminea by 1.3, 1.5, and 1.7%, respectively. The G+C content was 62.8 mol%, similar to the 62.7-63 mol% reported for P. flavescens but slightly higher than the 62.5-62.6 mol% of P. straminea and significantly higher than the 57.5-58.0 mol% of P. argentinensis. The major cellular fatty acids present in the CA5^sup T^ strain were C^sub 18:1^ ω7c (41.1%), C^sub 16:1^ ω6c and C^sub 16:1^ ω7c (25.7%), C^sub 16:0^ (12.0%), C^sub 12:0^ (8.0%), C^sub 12:0^ 3-OH (4.4%), and C^sub 10:0^ 3-OH (2.9%). The cellular fatty acid profile, GC content, phenotypic properties, and biochemical characteristics were consistent with its placement within the genus Pseudomonas. The name P. seleniipraecipitatus is proposed for these bacteria.[PUBLICATION ABSTRACT]