Abstract

The results showed that the plasmid copy number decreased proportionally with the amount of pA3 transfected in the presence or absence of filler DNA in both CHO and HEK cells (data not shown). [...] filler DNA did not influence the delivery of coding pDNA to transfected cells.

Details

Title
The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells
Author
Kiseljak, Divor; Rajendra, Yashas; Manoli, Sagar S; Baldi, Lucia; Hacker, David L; Wurm, Florian M
First page
P33
Publication year
2011
Publication date
2011
Publisher
BioMed Central
e-ISSN
1753-6561
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/1753-6561-5-S8-P33
ProQuest document ID
905245393
Copyright
© 2011 Kiseljak et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.