Abstract

Abstract: This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum . This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.

Details

Title
An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum
Author
Hasenkamp, Sandra; Wong, Eleanor H; Horrocks, Paul
Pages
42
Publication year
2012
Publication date
2012
Publisher
BioMed Central
e-ISSN
14752875
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
926073891
Copyright
© 2012 Hasenkamp et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.