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Arch Virol (2012) 157:733738 DOI 10.1007/s00705-011-1210-x
BRIEF REPORT
Genetic characterization of Pseudomonas aeruginosa bacteriophage KPP10
Jumpei Uchiyama Mohammad Rashel Iyo Takemura
Shin-ichiro Kato Takako Ujihara Asako Muraoka
Shigenobu Matsuzaki Masanori Daibata
Received: 25 September 2011 / Accepted: 24 November 2011 / Published online: 5 January 2012 Springer-Verlag 2011
Abstract Bacteriophage (phage) KPP10 has been used in experimental phage therapies directed against P. aeruginosa infections. To examine the eligibility of phage KPP10 as a therapeutic phage, its genome was analyzed. The genomic DNA was shown to be 88,322 bp long, with 158 open reading frames (ORFs), and three tRNA genes were predicted. No ORF-encoded pathogenicity or lysogenization factor was predicted. A comparative genomic analysis revealed that phage KPP10, together with phage PAK_P3, can be grouped as a new type of lytic phage infectingP. aeruginosa. Phage KPP10 is considered to be suitable for therapeutic purposes because it is a lytic phage without ORF-encoded pathogenicity or a lysogenization factors.
Keywords Pseudomonas aeruginosa Bacteriophage
Therapeutic phage Genomes
Pseudomonas aeruginosa is a Gram-negative aerobic rod and is part of the bacterial ora in the environment and the human body. However, some strains become highly resistant to many antibiotics and produce biolms [14]. Therefore, in clinical settings, they neutralize conventional chemotherapies and signicantly affect individuals with compromised immune systems.
The bacteriophages (phages) that infect P. aeruginosa have been studied well in terms of their horizontal transfer of pathogenic genes and their role in the development of phage therapy for intractable infections [6, 11, 17, 20, 25, 27]. Phage KPP10, which has been classied as a member of the family Myoviridae, morphotype A1, has strong lytic activity [25, 27]. It has been used as a therapeutic phage to treat gut-derived sepsis in mice infected with P. aeruginosa, with signicant therapeutic outcomes [27]. However, the genetic characteristics of phage KPP10 have been negligibly documented in previous studies [25, 27]. Therefore, we undertook the genetic characterization of phage KPP10, followed by comparative genomic and phylogenetic analysis.
Phage KPP10 was isolated from the river in Kochi, Japan. P. aeruginosa strain PA20, which was isolated from Kochi University Hospital, Kochi, Japan, was used as the host strain for the amplication of phage KPP10 [27]. TheP. aeruginosa strains used in this study are described in Supplementary Table...